American Journal of Biochemistry and Molecular Biology2150-42102150-4253Academic Journals Inc.10.3923/ajbmb.2022.1.12Aristolochia bracteolata on Diethylnitrosamine Induced Hepatocellular Carcinoma in Rats]]>GabrielI.B. ChristopherK. UmaruH.A. DahiruD. 12022121Background and Objective: Diethylnitrosamine (DEN) is a well-known liver carcinogen in rats, forming DNA adducts in the liver and inducing hepatocellular damage. The present study was focused on anticancer and antioxidant effects of methanol leaf extract of Aristolochia bracteolata and its fractions against diethylnitrosamine induced hepatocarcinoma in rats. Materials and Methods: Hepatocellular damage was induced using diethylnitrosamine orally. Hepatocellular damage was assessed using biochemical parameters of the liver function tests, antioxidant enzymes which are considered as biomarkers in hepatocarcinoma. Results: Diethylnitrosamine caused significant hepatocellular damage with liver enzymes (Apatite transaminases, alanine transaminases and alkaline phosphatase activities) of 381±0.84, 125±0.37, 510±0.51 and 293±1.45, 75±1.57, 274±1.71 compared to the control groups at p<0.05, respectively. However, treatments with methanol leaf extract of Aristolochia bracteolata and its fractions as well as Silymarin significantly reverse the liver damage 284±2.88, 298±4.26 298±1.57 for different doses of the plant extract and fractions and Silymarin 295±0.71, respectively. 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