American Journal of Biochemistry and Molecular Biology2150-42102150-4253Academic Journals Inc.10.3923/ajbmb.2017.21.40Clarias gariepinus (Burchell, 1822)]]>MekkawyImam A.A. MahmoudU.M. SalahSara 1201771Background and Objective: Atrazine is considered the most common aquatic herbicide used. So, the present study aimed at studying atrazine-induced changes in some biochemical characteristics during early embryonic stages of Clarias gariepinus . These characteristics include two metabolic enzymes, lactate hydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G6PDH), two oxidative enzymes, glutathione reductase (GSH) and superoxide dismutase (SOD), two thyroid hormones, triiodothyronine (T3) and thyroxine (T4), lipid peroxidation (LPO), DNA damage and patterns of protein fractions of different early developmental stages. Material and Methods: Gametes were collected from mature specimens of the African catfish, C. gariepinus from the river Nile. The fertilized eggs were incubated in dechlorinized tap water (pH = 7.7, dissolved oxygen 88-94% saturation, temperature 27-29EC and photoperiod 12:12 (light:dark). Under atrazine influences, different measurements of enzyme activities, electrophoretic protein analysis, lipid peroxidation, DNA fragmentation, Total Protein (TP) and hormones were carried out. Results: The atrazine-induced significant fluctuation in the activity of G6PDH and LDH with a sharp decrease in 24 h-PFS after hatching. There is no significant variations in GSH activity under atrazine doses except in 24 and 96 h-PFS with a decrease in all developmental stages except at 72 h-PFS. Insignificant fluctuation in SOD activity in all developmental stages of C. gariepinus except 4, 20 and 24 h-PFS in comparison to control. Atrazine induced significant fluctuation in DNA damage and LPO/TP ratio in all developmental stages. Insignificant fluctuation in T3 activity but significant decrease in T4 activity in all developmental stages of C. gariepinus under exposure of sublethal doses of atrazine. Conclusion: One could conclude that atrazine significantly disturbe the metabolic parameters of embryonic and developmental stages of fishes and in turn most of these parameters could be used as indicators of environmental aquatic pollution.]]>El-Elaimy, I.A., M.M. El-Saadany, S.A. Gabr and S.A. Sakr,1990Tilapia nilotica under stress of exposure to diazinon and neopybuthrin.]]>1223236Wodageneh, A.,20012001Qian, H., W. Chen, G.D. Sheng, X. Xu, W. Liu and Z. Fu,2008Chlorella vulgaris.]]>88301307Nwani, C.D., W.S. Lakra, N.S. Nagpure, R. Kumar, B. Kushwaha and S.K. Srivastava,2010Channa punctatus (Bloch).]]>732983312Farombi, E.O., Y.R. Ajimoko and O.A. Adelowo,2008Clarias gariepinus).]]>5423427Benarji, G. and T. Rajendranath,1990Clarias batrachus (Linnaeus).]]>2197202Samprath, K., S. Velamnial, I.J. Kennedy and R. James,1993Oreochromis mossambicus as a function of exposure period and sublethal levels of Ekalux.]]>357383Singh, N.N. and A.K. Srivastava,1994Heteropneustes fossilis.]]>4137140Adedeji, O.B., O.K. Adeyemo and S.A. Agbede,2009Clarias gariepinus).]]>839403946Paskova, V., K. Hilscherova and L. Blaha,20112112561Khan, M.Z. and F.C.P. Law,200542315323Devlin, R.H. and Y. Nagahama,2002208191364Almeida, J.A., E.L.B. Novelli, M. Dal pPai Silva and R. Alves Jr.,2001Oreochromis niloticus.]]>114169175Tongo, I., L. Ezemonye and U. Ochei,2012Bufo regularis).]]>21823Manning, T.,200511152Bjorkblom, C.,20092009Luskova, V., M. Svoboda and J. Kolarova,2002Cyprinus carpio L.).]]>71117123Bahnasawy, M.H.,2001599125Zaki, M.S., O.M. Fawzi, S.O. Mostafa, I. Awad and M. Fawzy,201039095Sobha, K., A. Poornima, P. Harini and K. Veeraiah,2007Catla catla (Hamilton) exposed to the heavy metal toxicant cadmium chloride.]]>1111Uttara, B., A.V. Singh, P. Zamboni and R.T. Mahajan,200976574Fang, Y.Z., S. Yang and G. Wu,200218872879Hsu, C.H., Z.H. Wen, C.S. Lin and C. Chakraborty,20074111120Garcia-Alonso, F.J., A. Guidarelli and M.J. Periago,2007tert-butylhydroperoxide in U937 cells: The role of iron chelation.]]>18457466Wang, C., Y. Zhao, R. Zheng, X. Ding, W. Wei, Z. Zuo and Y. Chen,2006Sebastiscus marmoratus.]]>65381387Mondon, J.A., S. Duda and B.F. Nowak,2001Rhombosolea tapirina to contaminated marine sediment and diet.]]>54231247Siroka, B., J. Huttova, L. Tamas, M. Simonovicova and I. Mistrik,200459513517Nguyen, L.T.H. and C.R. Janssen,2002Clarias gariepinus): Comparative sensitivity of endpoints.]]>42256262Mekkawy, I.A.A., U.M. Mahmoud, A.G. Osman and A.E.D.H. Sayed,2010Clarias gariepinus (Burchell, 1822).]]>36605626Sayed, A.E.D.H., U.M. Mahmoud and I.A. Mekkawy,2012Clarias gariepinus exposed to 4-nonylphenol.]]>78310319Sayed, A.E.D.H., I.A.A. Mekkawy and U.M. Mahmoud,2011Clarias gariepinus (Burchell, 1822).]]>5287297Sayed, A.E.D.H., A.T. Ibrahim, I.A.A. Mekkawy and U.M. Mahmoud,2007Clarias gariepinus (Burchell, 1822).]]>89170174Osman, A.G.M., I.A. Mekkawy, J. Verreth and F. Kirschbaum,2007Clarias gariepinus (Burchell, 1822).]]>33113Richter, C.J.J., W.J.A.R. Viveen, E.H. Eding, M. Sukkel and A.J. Rothuis et al.,1987Clarias gariepinus, kept in subtropical ponds in Israel and under Israeli and Dutch hatchery conditions.]]>63169185Osman, A.G.M., M. Koutb and A.E.D.H. Sayed,2010Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet-A radiation on African catfish Clarias gariepinus (Burchell, 1822).]]>9918Peter, M.C.S., J. Leji, V. Rejitha, J. Ignatius and V.S. Peter,2008Clarias gariepinus) to water-borne ferric iron: Effects on thyroidal, metabolic and hydromineral regulations.]]>122430Osman, A.G.M., S. Wuertz, I.A. Mekkawy, H.J. Exner and F. Kirschbaum,2007Clarias gariepinus (Burchell, 1822).]]>22375389Weis, J.S. and P. Weis,1987717785Mahmoud, U.M., I.A.A. Mekkawy and A.E.D.H. Sayed,2009Clarias gariepinus (Burchell, 1822).]]>95117128Mekkawy, I.A.A. and F.E. Lashein,2003Ctenopharyngodon idellus with emphasis on the corresponding morphological variations.]]>2003Sayed, A.E.D.H., I.A. Mekkawy and U.M. Mahmoud,2011Clarias gariepinus (Burchell; 1822).]]>2011De Graaf, G. and H. Janssen,1996Clarias gariepinus in Sub Saharan Africa.]]>1996Lemos, D., M. Salomon, V. Gomes, V.N. Phan and F. Buchholz,2003Farfantepenaeus paulensis (Crustacea, Decapoda, Penaeidae): Effects of development and temperature.]]>135707719Berges, J.A. and J.S. Ballantyne,19914823852394Kachmar, J.F. and D.W. Moss,19761976pp: 666-672pp: 666-672Kornberg, A.,19551955pp: 441-443pp: 441-443Aellig, A., A. Albert, G. Blin, J. Buret and E. Daubrosse et al.,198240697708Goldberg, D. and R. Spooner,19833rd Edn.,Nishikimi, M., N.A. Rao and K. Yagi,197246849854Laemmli, U.K.,1970227680685Gornall, A.G., C.J. Bardawill and M.M. David,1949177751766Utley, H.G., F. Bernheim and P. Hochstein,19671182932Buege, J.A. and S.D. Aust,19781978pp: 302-310pp: 302-310Kurita-Ochiai, T., K. Fukushima and K. Ochiai,1999672229Cobb, W.E., R.P. Lamberton and I.M. Jackson,19843015581560Skelley, D.S., L.P. Brown and P.K. Besch,197319146186SPSS.,1998Baxter, L., R.A. Brain, A.J. Hosmer, M. Nema, K.M. Muller, K.R. Solomon and M.L. Hanson,2015Ambystoma maculatum) and its endosymbiotic alga (Oophila amblystomatis).]]>206324331Ji, Q., J. Lee, Y.H. Lin, G. Jing and L.J. Tsai et al.,2016Xenopus laevis oocytes and have an adverse effect on early embryo development.]]>326369Liu, Z., Y. Wang, Z. Zhu, E. Yang, X. Feng, Z. Fu and Y. Jin,2016Danio rerio).]]>148163170MacLoughlin, C., I.S. Canosa, G.R. Silveyra, L.S.L. Greco and E.M. Rodriguez,2016Cherax quadricarinatus.]]>13196103Liu, Z., Z. Fu and Y. Jin,2017Danio rerio).]]>166212220Farah, M.A., B. Ateeq, M.N. Ali, R. Sabir and W. Ahmad,200455257265Pandey, S., R. Kumar, S. Sharma, N.S. Naghpure, S.K. Srivastava and M.S. Verma,2005Channa punctatus (Bloch).]]>61114120Ayoola, S.O.,2008Oreochromis niloticus) juvenile.]]>3825834Costa, M.J., D.A. Monteiro, A.L. Oliveira-Neto, F.T. Rantin and A.L. Kalinin,2008®.]]>17153163Do Carmo Langiano, V. and C.B.R. Martinez,2008Prochilodus lineatus.]]>147222231Lushchak, O.V., O.I. Kubrak, M.J. Storey, B.K. Storey and I.V. Lushchak,200976932937Kumar, A., M.B. Tyagi and P.N. Jha,200431810251030Oulmi, Y., R.D. Negele and T. Braunbeck,1995Oncorhynchus mykiss) renal tubules following prolonged exposure to sublethal concentrations of atrazine.]]>323950Ramel, F., C. Sulmon, M. Bogard, I. Couee and G. Gouesbet,2009Arabidopsis thaliana plantlets.]]>2009Wiegand, C., E. Krause, C. Steinberg and S. Pflugmacher,2001Danio rerio).]]>49199205Kumaran, S.S., C. Kavitha, M. Ramesh and T. Grummt,2011Clarias gariepinus.]]>31752761Mekkawy, I.A.A., U.M. Mahmoud, E.T. Wassif and M. Naguib,2011Oreochromis niloticus (Linnaeus, 1758) dietary supplemented with tomato paste and vitamin E.]]>377184Thapar, A., R. Sandhir and R. Kiran,200240963966Singh, M., R. Sandhir and R. Kiran,200644580583Doubek, J., S. Slosarkova, K. Rehakova, J. Bouda and P. Scheer et al.,20102010Yildirim, N.C., F. Benzer and D. Danabas,2011Capoeta trutta (Heckel, 1843).]]>216671Trenzado, C., M.C. Hidalgo, M. Garcia-Gallego, A.E. Morales and M. Furne et al.,2006Acipenser naccarii and trout Oncorhynchus mykiss. A comparative study.]]>254758767Velkova-Jordanoska, L., G. Kostoski and B. Jordanoska,200814235237Borkovic-Mitic, S.S., T.B. Kovacevic, B.R. Perendija, S.G. Despotovic, J.P. Gavric, S.Z. Pavlovic and Z.S. Saicic,2011Unio pictorum from the Sava River.]]>63185192An, A., Y. Li, X. Niu and H. Yu,2008599105Vinodhini, R. and M. Narayanan,2009Cyprinus carpio L.) after heavy metal exposure.]]>33273278Modesto, K.A. and C.B.R. Martinez,201081781787Giguere, A., P.G.C. Campbell, L. Hare and C. Cossu-Leguille,2005Perca flavescens) collected from eight lakes along a metal contamination gradient (Cd, Cu, Zn, Ni).]]>62563577Gad, N.S.,2011Oreochromis niloticus as biomarkers of exposure to crude oil pollution.]]>14958John, S., M. Kale, N. Rathore and D. Bhatnagar,200112500504Datta, C., J. Gupta, A. Sarkar and D. Sengupta,1992306570St. Dimitrova, M., V. Tishinova and V. Velcheva,1994Cyprinus carpio).]]>1084346Jayachandran, K., K. Pugazhendy, M. Susiladevi and C. Jayanthi,2010Labeo rohita (Hamilton).]]>51520Haluzova, I., H. Modra, J. Blahova, M. Havelkova, Z. Siroka and Z. Svobodova,201148589Ferreira, D., T.C. Unfer, H.C. Rocha, L.C. Kreutz, G. Koakoski and L.J.G. Barcellos,201210215220Parthasarathy, R. and J. Joseph,2011Oreochromis mossambicus).]]>5530534Ceyhun, S.B., M. Senterk, D. Ekinci, O. Erdogan, A. Ciltas and E.M. Kocaman,2010152215223Dorval, J. and A. Hontela,2003Oncorhynchus mykiss).]]>192191200Prasanthi, K., Muralidhara and P.S. Rajini,200543299306Rodrigues, E.D.L. and E. Fanta,1998Brachydanio rerio Hamilton-Buchman after acute exposure to sublethal levels of the organophosphate Dimethoate 500.]]>15441450Machado, M.R. and E. Fanta,2003Metynnis roosevelti.]]>46361372Yamano, T. and S. Morita,19952817Yildirim, N.C., M. Yurekli and N. Yildirim,201035140144Nammalwar, P.,19921992pp: 359-370pp: 359-370Zhang, J., H. Shen, X. Wang, J. Wu and Y. Xue,2004Carassius auratus.]]>55167174El-Demerdash, F.M., M.I. Yousef, F.S. Kedwanyand and H.H. Baghdadi,200493443459Sharma, Y., S. Bashir, M. Irshad, T.C. Nagc and T.D. Dogra,2005215173181Kamboj, A., R. Kiran and R. Sandhir,2006170567575Atif, F., S. Parvez, S. Pandey, M. Ali and M. Kaur et al.,2005Channa punctata Bloch.]]>49371377Koprucu, S.S., E. Yonar and E. Seker,2008Unio elongatulus eucirrus.]]>81253257Peebua, P., P. Kosiyachinda, P. Pokethitiyook and M. Kruatrachue,2007Oreochromis niloticus) using biochemical biomarkers.]]>78138141Chandra, S.,2008Heteropneustes fossilis.]]>94552Singh, M., R. Sandhir and R. Kiran,201163269276Cooke, M.S., M.D. Evans, M. Dizdaroglu and J. Lunec,20031711951214Schmutzler, C., I. Gotthardt, P. Hofmann, B. Radovic and G. Kovacs et al.,2007in vitro and in vivo analysis of potential new biomarkers.]]>1157783Leatherland, J.F.,19942167Peter, M.C.S., R.A.C. Lock and S.E.W. Bonga,2000Oreochromis mossambicus.]]>120157167Oommen, O.V., P. Sreejith, R.S. Beyo, L. Divya, A.S. Vijayasree and M. Manju,20061096105Leji, J., G.S. Babitha, V. Rejitha, A.S. Vijayasree and J. Ignatius et al.,2007Oreochromis mossambicus) to the effluents of coconut husk retting.]]>112330Peter, M.C.S.,20072007Peter, V.S. and M.C.S. Peter,2007Anabas testudineus Bloch.]]>116268Blanton, M.L. and J.L. Specker,20073797115Gorbman, A.,19691969Eales, J.G.,19791979pp: 341-436pp: 341-436Eales, J.G.,19856312171231Grau, E.G.,198828329335Power, D.M., L. Llewellyn, M. Faustino, M.A. Nowell and B.T. Bjornsson et al.,2001130446459Mylonas, C.C., C.V. Sullivan and J.M. Hinshaw,1994Salmo trutta) reproduction and early development.]]>13485493Reddy, P.K., C.L. Brown, J.F. Leatherland and T.J. Lam,1992Oreochromis mossambicus): II. Changes in the hormones and 5'-monodeiodinase activity during development.]]>9487496Weber, G.M., D.K. Okimoto, N.H. Richman III and E.G. Grau,1992Oreochromis mossambicus, during oogenesis.]]>85392404Ayson, F.G. and T.J. Lam,1993Siganus guttatus) broodstock: Changes in thyroid hormone levels in plasma, eggs and yolk-sac larvae and its effect on larval growth and survival.]]>1098393Tagawa, M., T. Ogasawara, T. Sakamoto, T. Miura, K. Yamauchi and T. Hirano,199413233240Brown, C.L., S.I. Doroshov, M.D. Cochran and H.A. Bern,19897295299Leatherland, J.F., L. Lin, N.E. Down and E.M. Donaldson,1989Oncorhynchus species.]]>4621402145Tagawa, M. and T. Hirano,1987Oncorhynchus keta.]]>68129135Sinha, N., B. Lal and T.P. Singh,1991Clarias batrachus.]]>100107110Yadav, A.K. and T.P. Singh,1986Heteropneustes fossilis (Bloch).]]>39136142Brown, S.B., D.L. MacLatchy, T.J. Hara and J.G. Eales,19903 and T4 in rainbow trout (Oncorhynchus mykiss).]]>6815371543Suchiang, P. and B.B.P. Gupta,2011Clarias gariepinus during different phases of the breeding cycle.]]>33242Oppenheimer, J.H., H.L. Schwartz, C.N. Mariash, W.B. Kinlaw, N.C.W. Wong and H.C. Freake,19878288308Eales, J.G.,198828351362Van der Geyten, S., J.P. Sanders, V.M. Darras, E.R. Kuhn, J.L. Leonard and T.J. Visser,1998839498499Reyns, G.E., K.A. Janssens, J. Buyse, E.R. Kuhn and V.M. Darras,2002132239245