American Journal of Biochemistry and Molecular Biology2150-42102150-4253Academic Journals Inc.10.3923/ajbmb.2016.67.71KumarAnil ChhokarVinod KumarRavinder AggarwalHimanshu BeniwalVikas 2201662Background: Triacylglycerols are quantitatively most important storage form of energy for eukaryotic cells. Acyl CoA: Diacylglycerol O-acyltransferase 1 (DGAT1, EC 2.3.1.20) is a microsomal enzyme that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Objective: The present study was undertaken with the objectives to study the molecular differences in DGAT1 among different ruminants. Materials and Methods: The DGAT1 was partially amplified (411 bp) by designing gene specific primers and confirmed by sequencing the amplicon and its comparison with DGAT1 gene of other ruminants (i.e., bovine, buffalo and goat). Results: Comparative study of DGAT1 among different ruminants reveals different level of mutation with respect to its gene sequence (0.0-0.4%) and protein sequence (0.0-1.2%). Conclusion: Molecular differentiation among different ruminants reveals that bovine and buffalo are very close to each other and sheep is related to goat. Further study is underway to detect polymorphism and associated them with milk fat trait in sheep.]]>Liu, Q., R.M.P. Siloto, R. Lehner, S.J. Stone and R.J. Weselake,201251350377Hiramine, Y. and T. Tanabe,201167259264Turchetto-Zolet, A.C., F.S. Maraschin, G.L. de Morais, A. Cagliari, C.M. Andrade, M. Margis-Pinheiro and R. Margis,20112011Winter, A., W. Kramer, F.A.O. Werner, S. Kollers and S. Kata et al.,20029993009305Boichard, D., C. Grohs, F. Bourgeois, F. Cerqueira and R. Faugeras et al.,20033577101Spelman, R.J., C.A. Ford, P. McElhinney, G.C. Gregory and R.G. Snell,20021235143517Raut, A.A., A. Kumar, S.N. Kala, V. Chhokar and N. Rana et al.,2012DGAT1 gene of buffaloes by PCR-SSCP.]]>35610613Lacorte, G.A., M.A. Machado, M.L. Martinez, A.L. Campos and R.P. Maciel et al.,20065475482Thaller, G., W. Kramer, A. Winter, B. Kaupe, G. Erhardt and R. Fries,20038119111918Kuhn, C., G. Thaller, A. Winter, O.R.P. Bininda-Emonds and B. Kaupe et al.,200416718731881Sambrook, J. and D.W. Russell,20013rd Edn.,Pages: 2344Pages: 2344Kumar, A., R. Kumar, V. Beniwal, S.N. Kala and A. Mishra et al.,2012Bubalus bubalis.]]>8600606Kumar, A., V. Chhokar, R. Kumar, V. Beniwal and S.N. Kala et al.,2012Bubalus bubalis.]]>3685694Grisart, B., F. Farnir, L. Karim, N. Cambisano and J.J. Kim et al.,200410123982403Mishra, B., M.S. Tantia, S.T.B. Kumar and R.K. Vijh,2007Bubalus bubalis).]]>3010971100Weller, J.I., M. Golik, E. Seroussi, E. Ezra and M. Ron,20038622192227Tantia, M.S., R.K. Vijh, B.P. Mishra, B.S.T. Mishra Kumar and M. Sodhi,2006Bos indicus) and buffalo (Bubalus bubalis) breeds.]]>2006Harris, C.A., J.T. Haas, R.S. Streeper, S.J. Stone and M. Kumari et al.,201152657667