American Journal of Biochemistry and Molecular Biology2150-42102150-4253Academic Journals Inc.10.3923/ajbmb.2013.91.100Gardnerella vaginalis Bacteriocin HV6b Produced by Lactobacillus fermentum Isolate from Human Vaginal Ecosystem]]>KaurBaljinder BalgirPraveen MittuBharti ChauhanAshish KumarBalvir 1201331Anti-Gardnerella vaginalis, Lactobacillus fermentum bacteriocin HV6b
producing strain was isolated from vaginal swabs of healthy, fecund females.
It was identified, purified and further characterized physicochemically. The
relative molecular mass of bacteriocin was found to be less than 7 kDa. Maximum
bacteriocin production was observed at 37°C, pH 6.5 after 16-18 h of incubation
Proteinase K and pepsin strongly inhibited bacteriocin production. The bacteriocin
has purified by absorption desorption method and characterized by reverse phase
liquid chromatography. It exhibited high thermal stability and showed inhibitory
activity over wide range of pH. The study revealed the possibility of using
bacteriocin for bio-medical use and the L. fermentum strain as probiotic.]]>Abdi, S., Y. Ourova and O. Menchi,20102147153Abd El-Hady, R.A.M.,201117486El-Hady, R.A.M.A. and R.A.A.A. El-Baky,201111426Adedayo, A.V.,20124112Amsel, R., P.A. Totten, C.A. Spiegel, K.C. Chen, D. Eschenbach and K.K. Holmes,1983741422Chauhan, A. and B. Kaith, 20122012Barber, M., R.S. Bordoli, R.D. Sedgewick and A.N. Tyler,198111325347Boris, S., R. Jimenez-Diaz, J.L. Caso and C . Barbes,2001Lactobacillus delbrueckii subsp. lactis UO004, an intestinal isolate with probiotic potential.]]>91328333Das, B.R., S.M. Istiaque and R.S. Rengasamy,201112734Ronnqvist, D., U. Forsgren-Brusk, U. Husmark and E. Grahn-Hakansson,2007Lactobacillus fermentum Ess-1 with unique growth inhibition of vulvo-vaginal candidiasis pathogens.]]>5615001504Hay, P.,20002506512Issaoui, C., A.H. Said and S. Roudesli,2011198105Kawai, Y., R. Kemperman, J. Kok and T. Saito,20045393398Kawai, Y., T. Saito, J. Uenur and T. Otoh,199761179182Klaenhammer, T.R., C. Fremaux, C. Ahn and K. Milton,19931993pp: 151-180pp: 151-180Tomas, M.S.J., V.S. Ocana, B. Wiese and M.E. Nader-Macias,2003Lactobacillus acidophilus CRL 1259 and inhibition of uropathogenic Escherichia coli.]]>5211171124Madhivanan, P., K. Krupp, J. Hardin, C. Karat, J.D. Klausner and A.L. Reingold,200914703708Maqueda, M., A. Galvez, M.M. Bueno, M.J.O.S.E. Sanchez-Barrena and C. Gonzalez et al.,20045399416Miteva, V., I. Ivanova, I. Budakov, A. Pantev and T. Stefanova,1998Lactobacillus delbrueckii strain 1043.]]>85603614Nagaraja, P.,2008Gardrenalla vaginalis in recurrent bacterial vaginosis.]]>26155157Pucci, M.J., E.R. Vedamuthu, B.S. Kunka and P.A. Vandenbergh,1988Listeria monocytogenes by using bacteriocin PA-1 produced by Pediococcus acidilactici PAC 1.0.]]>5423492353Raja, A.S.M. and G. Thilagavathi,20111138144Rocco, F.,201114249Aan, M.P.S., M. Krishna, H.N.N. Murthy and S.K. Rai,201132030Tagg, J.R., A.S. Dajani and L.W. Wannamaker,197640722756Toba, T., E. Yoshioka and T. Itoh,1991Lactobacillus acidophilus LAPT 1060.]]>12106108Yang, R., M.C. Johnson and B. Ray,19925833553359Wu, Y., M.M. Nelson, A. Quaile and D. Xia,2009Plasmodium falciparum.]]>8105112