American Journal of Biochemistry and Molecular Biology2150-42102150-4253Academic Journals Inc.10.3923/ajbmb.2013.110.118N. AnyasorGodswill M. OdunaikeOluwadamilola O. OlorunsogoOlufunso 1201331This study investigated the cytotoxic effect of varying doses of antimalarial coartemether (2.0, 4.0, 8.0 and 10.0 mg kg-1) in the presence of ferrous sulphate (2800 mg kg-1) for 3 days on normal rat liver mitochondrial membrane permeability transition pore opening and F1F0 ATPase activity. Swelling was estimated spectrophotometrically under succinate energized condition. Calcium ion treated mitochondria preloaded with coartemether induced swelling in a concentration dependent manner in vitro. Swelling was amplified in the presence of ferrous. Coartemether alone and combined coartemether-ferrous also induced mitochondrial swelling in the presence of spermine. In vivo study further showed that 10.0 mg kg-1 coartemether induced swelling in the presence of ferrous. Coartemether stimulated an increased activity of mitochondrial F1F0 ATPase in a concentration dependent manner. Thus, these findings indicate that coartemether at high dose in the presence of ferrous sulphate could be an inducer of mitochondrial mega pore opening and an uncoupler of oxidative phosphorylation initiating apoptosis.]]>Ajibade, A.J., P.B. Fakunle and P.D. Shallie,20112011Bababunmi, E.A. and O. Bassir,197226102104Balint, G.A.,200190261265Brand, M.D. and D.G. Nicholis,2011435297312Byakika-Kibwika, P., M. Lamorde, H. Mayanja-Kizza, C. Merry, B. Colebunders and J. van Geertruyden,201061120Cumming, J.N., P. Ploypradith and G.H. Posner,199637253297Dhingra, V., K.V. Rao and M.L. Narasu,199966279300Eckstein-Ludwig, U., R.J. Webb, I.D.A. van Goethem, J.M. East and A.G. Lee et al.,2003Plasmodium falciparum.]]>424957961Efferth, T. and B. Kaina,201040405421Fiske, C.H. and Y. Subbarow,192566375400Galluzi, L., E. Morselli, O. Kepp and G. Kroemer,20091787402413Gunter, T.E. and D.R. Pfeifer,1990258C755C786Hietala, S.F., A. Martensson, B. Ngasala, S. Dahlstrom and N. Lindegardh et al.,20105447804788Krishna, S., A.C. Uhlemann and R.K. Haynes,20047233244Lapidus, R.G. and P.M. Sokolove,1993306246253Lardy, H.A. and H. Wellman,1953201357370Li, W., W. Mo, D. Shen, L. Sun and J. Wang et al.,20052005Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Moore, J.C., H. Lai, J.R. Li, R.L. Ren, J.A. McDougall, N.P. Singh and C.K. Chou,1995988387Mpiana, P.T., B.K. Mavakala and Y. Zhi-Wu,20073302310Mwesigwa, J., S. Parikh, B. McGee, P. German and T. Drysdale et al.,2010545259Nelson, D.L. and M.M. Cox,20085th Edn.,pp: 707-772pp: 707-772Noori, S., G.A. Naderi, Z.M. Hassan, Z. Habibi, S.Z. Bathaie and S.M.M. Hashemi,2004Artemisia annua on DTH responses compared with cyclosporine A.]]>413011306Oyemitan, I.A., E.O. Iwalewa, O.E. Ukponmwan, R.O. Akomolafe and O.M. Daniyan,20077575578Onyesom, I. and J.E. Agho,2011Plasmodium falciparum malarial patients in Edo-delta Region of Nigeria.]]>47883del Pilar Crespo, M., T.D. Avery, E. Hanssen, E. Fox and T.V. Robinson et al.,20085298109Premji, Z.G.,2009®: The journey to the clinic.]]>2009Raji, Y., I.O. Osonuga, O.S. Akinsomisoye, O.A. Osonuga and O.O. Mewoyeka,20055303306Schneider, W.C. and G.H. Hogeboom,1950183123128Skulachev, V.P.,199629169202Zoratti, M. and I. Szabo,19951241139176Vinogradov, A.D.,20001F0 ATPase. Is ATP synthase a reversible molecular machine.]]>2034149Wang, J., L. Huang, J. Li, Q. Fan, Y. Long, Y. Li and B. Zhou,20102010WHO,2006Zablockaite, D., V. Gendviliene, I. Martisiene and J. Jurevicius,2007528993Zamzami, N., P. Marchetti, M. Castedo, C. Zanin, J.L. Vayssiere, P.X. Petit and G. Kroemer,1995in vivo.]]>18116611672Zorov, D.B., K.W. Kinnally and H. Tedeschi,199224119124Novartis Pharma AG.,2009® prescribing information.]]>2009Cousin, M., S. Kummerer, G. Lefevre, A.C. Marrast, D. Stein and M. Weaver,20082008