American Journal of Biochemistry and Molecular Biology2150-42102150-4253Academic Journals Inc.10.3923/ajbmb.2011.337.348 Pectinophora gossypiella (Saunders)]]>Abd-ElhadyHany K. Abd El-AalAhmed A. 4201114In Egypt pink bollworm Pectinophora gossypiella (Lepidoptera: Gelechiidae) has recently developed resistance to various kinds of insecticide in the field. The resistance status of pink bollworm to several classes of insecticides currently used in cotton fields was evaluated in the Nile Delta (Kafr-Elsheikh and Menoufia) Egypt. Two field-collected P. gossypiella populations have developed resistance to several classes of insecticides. Toxicological bioassays were conducted in the laboratory, LC50s estimated by probit analysis and Resistance Ratio (RR) were calculated at the LC50 level. Of the seven insecticides tested four insecticides (chlorpyrifos, profenofos, thiodicarb and carbaryl) showed the Resistance Ratio (RR) values above 20 folds, against two field-collected populations whereas the other three insecticides (fenpropathrin, spinosad and abamectin) had RR values less than 10 folds. Also, the patterns of selection pressure by abamectin at the LC30 level to Kafr-Elsheikh (KFR)-and Menoufia (MNF)-field strains were determined by continuous insecticide application under laboratory conditions for eight successive generations. On the other hand, biochemical assays indicated that both KFR-and MNF-field strains expressed higher levels of Acetylcholinesterase (AChE) activity by 4.1 and 2.8-times, respectively, compared to that of susceptible strain. There were significant differences in esterase activities among the field-collected populations and susceptible strain. Analysis of protein patterns by (SDS-PAGE) showed some differences between resistant strains to abamectin, MNF-and KFR-field strains and the bands differed in intensity and molecular weights between all strains. These findings indicate that rapid biochemical assays coupled with biological assays are potential tools for estimating both the intensity of resistance in the field.]]>Abbott, W.S.,192518265267Abdeen, S.A.,19851985Ahmad, M., A.H. Sayyed, M.A. Saleem and M. Ahmad,2008Spodoptera litura (Lepidoptera: Noctuidae) from Pakistan.]]>2713671372Ahmad, M., M.I. Arif and Z. Ahmad,2003Helicoverpa armigera (Lepidoptera: Noctuidae) to new chemistries in Pakistan.]]>22539544Attique, M.R., M.M. Ahmad, Z. Ahmad and M. Rafiq,2001Pectinophora gossypiella (Saunders) in the Punjab, Pakistan.]]>20421426Attique, M.R., Z. Ahmad, A.I. Mohyuddin and M.M. Ahmad,2001Pectinophora gossypiella (Saunders) and its control strategy in the Punjab, Pakistan.]]>33115123Baldwin, J.L. and J.B. Graves,1991pp: 1829pp: 1829Bradford, M.M.,197672248254Brown, T.M., P.K. Bryson, F. Arnette, M. Roof, I.L. Mallett, J.B. Graves and S.R. Nemec,1996Heliothis virescens.]]>1996pp: 149-157pp: 149-157Bues, R., J.C. Bouvier and L. Boudinhon,2005Helicoverpa armigera (Lepidoptera: Noctuidae) in the South of France.]]>24814820Byrne, F.J., K.L. Gorman, M. Cahill, I. Denholm and A.L. Devonshire,2000Bemisia tabaci (Genn).]]>56867874Christie, P.T. and D.J. Wright,1990Spodoptera littoralis boisduval and Heliothis armigera hubner (Lepidoptera: Noctuidae) and possible mechanisms determining differential toxicity.]]>292938Clark, J. M.,201046368CoStat Program,2006Dunley, J.E. and S.C. Welter,200093955962Eid, A.M.H., A.I. Gadallah, S.A. Abo-Donia and M.F.A. Abdel-Lateef,1979S. littoralis (Biosad) using acrlamid gel electrophorasis.]]>15556Abd El-Hafez, A., A.G. Metwally and M.R.A. Saleh,1982Pectinophora gossypiella (Saund.) on kidney bean diet in Egypt.]]>576110Ellman, G.L., K.D. Courtney, V. Andres Jr. and R.M. Featherstone,196178890Abd El-Mageed, A.E.M., E.M. Anwar, L.R.A. Elgohary and H.F. Dahi,2007493103Farghaly, S.F.,2010Bemisia tabaci.]]>8383389Finney, D.J.,19713rd Edn.,pp: 76-80pp: 76-80Gunning, R.V. and M.E. Balfe,2002Helicoverpa armigera (Lepidoptera: Noctuidae).]]>2002Harold, J.A. and J.A. Ottea,1997Heliothis virescens (F.).]]>582333Hewady, M.A.,1990Earias insulana (Boisd.) to certain noval insecticides with reference to resistance development.]]>1990Jongsma, M.A., J. Peters, W.J. Stiekema and D. Bosh,1996Spodoptera exigua Hubner (Lepidoptera: Noctuidae).]]>26185193Kakani, E.G., N.E. Zygouridis, K.T. Tsoumani, N. Seraphides, F.G. Zalom and K.D. Mathiopoulos,2010Bactrocera oleae (Diptera: Tephritidae) populations in California.]]>66447453Korejo, A.K., A.W. Soomro, G.H. Mallah, A.A. Memon and M.Y. Solangi,2000321192121Kranthi, K.R., D.R. Jadhav, S. Kranthi, R.R. Wanjari, S.S. Ali and D.A. Russell,200221449460Laemmli, U.K.,1970227680685Margaritopoulos, J.T., G. Skavdis, N. Kalogiannis, D. Nikou and E. Morou et al.,2008Bactrocera oleae populations from Greece and improved diagnostic for an iAChE mutation.]]>64900908Massoud, M.A., A.K. Mourad, B.A. El and S.M. El,2006Pectinophora gossypiella (Saund.) (Lepidoptera: Gelechiidae) in Egypt.]]>71517535Mirmoayedi, A., M. Maniee and A. Yaghutipoor,2010Earias insulana boisduval, using three Bio-Insecticides, Bt, Spinosad and Neem-Azal.]]>78994Osman, A.A., T.F. Watson and S. Sivasupramanium,199184353357Osorio, A., A.M. Martınez, M.I. Schneider, O. Diaz and J.L. Corrales et al.,20086410011007Plapp, Jr. F.W., C. Campanhola, R.D. Bagwell and B.F. McCutcheon,19901990pp: 237-260pp: 237-260Prabhaker, N., N.C. Toscano, T.J. Henneberry, S.J. Castle and D. Weddle,199689805815Rauch, N. and R. Nauen,2003Bemisia tabaci (Hemiptera: Aleyrodidae).]]>54165176Roditakis, E., M. Grispou, E. Morou, J.B. Kristoffersen and N. Roditakis et al.,200965313322Sayyed, A.H., M.N.R. Attique, A. Khaliq and D.J. Wright,2005Plutella xylostella (Lepidoptera: Plutellidae) from Pakistan.]]>61636642Shah, R., K. Armstrong, S.P. Worner and R.B. Chapman,2002510701073Soderlund, D.M. and J.R. Blopmquist,19901990pp: 58-96pp: 58-96Soleno, J., L. Anguiano, A.P. de D'Angelo, L. Cichon, D. Fernandez and C. Montagna,2008Cydia pomonella L. (Lepidoptera: Tortricidae) among orchards from the Argentinian Patagonia.]]>64964970Torres-Vila, L.M., M.C. Rodriguez-Molina, A. Lacasa-Plasencia and P. Bielza-Lino,2002Helicoverpa armigera to endosulfan, carbamates and organophosphates: The Spanish case.]]>2110031013Unlu, L.,2004720312033Van Asperen, K.,19628401416Wyss, C.F., H.P. Young, J. Shukla and R.M. Roe,2003Heliothis virescens (Lepidoptera: Noctuidae), highly resistant to spinosad.]]>22307314Yang, Y., M. Zhu and D. Wang,2000Pectinophora gosspiella saunders.]]>169296