Asian Journal of Biotechnology1996-0700xxxx-xxxxKnowledgia Review10.3923/ajbkr.2015.60.70Bordetella pertussis Strain 509 During Large Scale Batch Fermentor Cultivation]]>ShivanandappaK.C. JagannathanS. LokeshS. VijayakumarR. ManiK.R. 2201572Studies have been undertaken to investigate, the effect of impeller number and its position upon the growth yield of Bordetella pertussis strain 509 during large scale batch cultivation. It was shown that, the maximum growth yield opacity was found to be 70 IOU mL1 at 48 h cultivation in single impeller fixed at the bottom position of fermentor vessel. Whereas the optimal and moderate culture opacity 60 and 50 IOU mL1 was observed during the two impellers mounted at two different positions on the shaft. Similarly the least growth yield was obtained during three impeller located at top, middle and bottom region of the shaft and the final opacity was found to be 30 IOU mL1 at the end of 48 h cultivation with an increasing of culture pH 8.02. However, in two impeller combination experimental study also showed moderate growth yield at 48 h could give to homogenized mixing and good aeration. Thus, the two different location of the impeller combination influences two vortex flows, enhances optimal growth rate, viz; the lower impeller act as gas disperser and creates radial velocity with higher rates of air dispersion, whereas the upper impeller pumps the broth towards down thus the combined agitation of the broth leads to good mixing and even transfer of Dissolved Oxygen (DO2) to the growing cells and found moderate bacterial growth. In the combination of three impellers mounted at three different positions experimental study shown that, lesser growth yield at the end of 48 h cultivation, due to higher forth accumulation.]]>Adler, I. and A. Fiechter,198615159CDC.,2002517376Cruickshank, R., J.P. Duguid and R.H.A. Swain,196511th Edn.,Pages: 1067Pages: 1067Finn, R.K.,195418254274Galit, S.R.L., N. Otsuka, Y. Furuse, D.J.V. Almonia and L.T. Sombrero et al.,2015Bordetella pertussis in the Philippines in 2012-2014.]]>352426Greenberg, D.P., C.H.W. von Konig and U. Heininger,200524S39S43Jayaraj, R., K. Vidhyacharan, K.C. Shivanandappa and J. Jayaprakash,2011Bordetella pertussis strain 509 in lab scale cultivation.]]>22227Kumaresan, T. and J.B. Joshi,2006115173193Kuzmanic, N., R. Zanetic and M. Akrap,200847663669Nasrallah, N., J. Legrand, A. Bensmaili and L. Nouri,20084721752183Neinow, A.W. and J.J. Ulbrecht,19851985pp: 203-237pp: 203-237Patwardhan, A.W. and J.B. Joshi,19993831313143Pittman, M.,19791401412Shivanandappa, K.C., S. Jagannathan, S. Pandiyarajan, R. Vijayakumar, T. Umadevi, J. Kalaiselvan and B. Sekar,2015Bordetella pertussis vaccine strain 134.]]>2015Shivanandappa, K.C., S. Jagannathan, S. Pandiyarajan, P. Tamilvanan, T. Umadevi, J. Kalaiselvan and B. Seker,2015Bordetella pertussis vaccine strain 134 employed indigenous technology.]]>2015Shivanandappa, K.C., K.R. Mani, S. Jagannathan and R. Vijayakumar,2015Bordetella pertussis strain 509 cell mass yield in baffle and vortex mode of agitation during large scale industrial fermentor cultivation.]]>2015Soons, Z.I.T.A., J.A. Voogt, G. van Straten and A.J.B. van Boxtel,2006Bordetella pertussis using adaptive control.]]>125252268Thalen, M., M. Venema, A. Dekker, L. Berwald and J. van den IJssel et al.,2006Bordetella pertussis: Metabolism and pertussis toxin production.]]>34289297Vardar-Sukan, F.,1986214044Wendelboe, A.M., A. Van Rie, S. Salmaso and J.A. Englund,200524S58S61WHO.,20072007Zhou, G. and S.M. Kresta,19964224762490