Asian Journal of Biotechnology1996-0700xxxx-xxxxKnowledgia Review10.3923/ajbkr.2011.460.469T. aestivum L. em thell.) Using RAPD Markers]]>KumarReetesh K. JaiswalSanjay K. VishwakarmaManish P. RaiVed UpadhyayPriti K. JoshiArun 5201135Evaluation of genetic diversity was made using RAPD as a genetic marker system in allohexaploid wheat (T. aestivum). A set of 30 RAPD markers were used for 43 wheat cultivars to examine their utility for detecting DNA polymorphism in genotypes and estimating genetic diversity among them. A total of 228 different RAPD bands were scored for the presence of bands among all the varieties. Out of 30 oligonucleotide primers used, 5 primers gave reproducible DNA product (bands) suitable for the establishment of genetic diversity among the varieties. The Genetic Similarity (GS) coefficient for all pairs of genotypes was ranged from 0.66 to 1.0. V 35 (HD 2747) and V 39 (HI 8498) had high coefficient of similarity (0.982) while, V 4 (VL 804) and V 6 (VL 809) showed less coefficient of similarity (0.646). It indicated that V 35 (HD 2747) and V 39 (HI 8498) carry closely related ancestry while the cultivars V 4 (VL 804) and V 6 (VL 809) are not close to each other. Present results revealed the potential of RAPD technique in determining genetic diversity and make suitable fingerprints for wheat cultivars.]]>Ahmed, M.F., M. Iqbal, M.S. Masood, M.A. Rabbani and M. Munir,2010Triticum aestivum L.) advanced breeding lines using RAPD and SDS-PAGE.]]>13110Beaumont, V.H., J. Mantet, T.R. Rocheford and J.M. Widholm,1996Zea mays L.).]]>93606612Bibi, S., M.U. Dahot, I.A. Khan, A. Khatri and M.H. Naqvi,2009Triticum aestivum) using Random Amplified Polymorphic DNA (RAPD) markers.]]>4110231027Chatrath, R., B. Mishra and J. Shoran,20062006pp: 5353DWR,20062006Graner, A., W.F. Ludwig and A.E. Melchinger,19943411991205Gupta, P.K., R.K. Varshney, P.C. Sharma and B. Ramesh,1999118369390Hernandez, P., A. Martin and G. Dorado,19995245253Prakash, S.P.J., K.R. Biji, S.M. Gomez, K.G. Murthy and R.C. Babu,2006Sorghum bicolour L. Moench) accessions using RAPD markers.]]>1109112Joshi, A.K., B. Mishra, R. Chatrath, G.O. Ferrara and R.P. Singh,2007157431446Turner, K.M., W.P. Hanage, C. Fraser, T.R. Connor and B.G. Spratt,200773030Khan, A.A., A. Iqbal, F.S. Awan and I.A. Khan,2010428996Nagarajan, S.,20058914671471Nilsson, N.O., C. Hallden, M. Hanson, A. Hjerdin and T. Sall,199740644651Noiton, D.A.M. and P.A. Alspach,1996121773782Ramshini, H., M.R. Nagavi and H. Alizadeh,20054123127Vierling, R.A. and H.T. Nguyen,199284835838Rohlf, F.J.,1993Version 1.80,Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen and R.W. Allard,19848180148018Schachermayr, G., H. Siedler, M.D. Gale, H. Winzeler, M. Winzeler and B. Keller,199488110115Sharma, H.C.,1995824364Sorrells, M.E. and W.A. Wilson,199737691697Subudhi, P.K. and N. Huang,1999Oryza sativa L.).]]>1304149Sudhakar, Y. and A.K. Joshi,1996Puccinia Recondita in two wheat crosses.]]>1158588Tyagi, S.D. and M.H. Khan,2010186974Upadhyay, P., V.K. Singh and C.N. Neeraja,2011Oryza sativa L.) varieties of India.]]>5130140Vidal, J.R., M. Coarer and A. Defontaine,1999109161172Waugh, R. and W. Powell,199210186191Williams, J.G.K., M.K. Hanafey, J.A. Rafalski and S.V. Tingey,1993218704740Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey,19901865316535FAO,2004Jaccard, P.,190844223270