Asian Journal of Biotechnology1996-0700xxxx-xxxxKnowledgia Review10.3923/ajbkr.2010.110.119In vitro Callus Induction and Shoot Multiplication from Nodal Explants and Leaves of Memecylon edule]]>ElavazhaganTamizhamudu D. ArunachalamKantha 2201022A protocol for callus induction and shoot multiplication was developed for leaf and nodal segments of Memecylon edule (Melastomataceae). Leaf and nodal explants were cultured on MS medium supplemented with different phytohormones combinations IAA, NAA, 2,4,D, BAP, KIN, GA3 with different concentrations (0.5-6 mg) for callus induction and shoot multiplication. Callus was initiated from cut ends of green tender leaves and nodal segments of Memecylon edule in both dark and light condition. Green friable callus and light brown calli was derived from nodal segments of MS ½ strength medium supplemented with 0.7% agar and 30% sucrose with GA3+BAP+NAA, BAP+NAA+KN+GA3, BAP+KN+NAA, BAP+GA3+NAA, BAP+GA3+2,4,D, IAA+NAA+ 2,4,D+KN+BAP, BAP+KN+NAA+2,4,D+GA3 promotes the callogenesis in nodal explants. A best combination for shoot multiplication was MS medium supplemented with IAA+BAP+GA3 from the nodal segments and the nodal calli and IAA+BAP combination with 1 to 3 mg concentration was best for leaf callus. Percentages of callus induction were higher in nodal segments than leaf. In both leaf and nodal explants callus was developed in the MS medium containing BAP with IAA and GA3 combinations.]]>Ranjit, D.R.J., V.S. Ramachandran, J. Vencatesan, V. Ramakantha and J.P. Puyravaud200792586588Nualkaew, S., S. Thongpraditchote, Y. Wongkrajang and K. Rattanamanee,2007Memecylon edule Roxb.]]>2007Steven, J., T. Watanabe and E. Smets,2002905364Natarajan, D., S.J. Britto, B. Balaguru, N. Nagamurugan, S. Soosairaj and D.I. Arockiasamy,20048613161323Kongkachuichai, P., A. Shitangkoon and N. Chinwongamorn,200253179185Luis, P.B.C., C. Adriane, M. Gomes, B.R.S. da Costa and J.B. Teixeira,1997Miconia sp., a woody Melastomataceae from Brazil, using thidiazuron as plant growth regulator.]]>92125Song, J.Z., L. Nali, K.L. Wu, Z.L. Chen and J. Duan,2008Tigridiopalma magnifica (Melastomataceae).]]>4011791184Sahoo, Y., S.K. Pattnaik and P.K. Chand,1997Moms indica L. derived from seedlings and mature plants.]]>698598Amoo, S.O. and B.E. Ayisire,2005Parkia biglobosa (Jacq.) Benth.]]>46871Amalraj, T. and S. Ignacimuthu,1998Memecylon umbellatum in normal and alloxan diabetic mice.]]>62247250Mujib, A.,2005In vitro regeneration of Sandal (Santalum album L.) from leaves.]]>296367Al-Khalifah, N.S.,20042004pp: 116Ahmed, M.B., M. Salahin, R. Karim, M.A. Razvy and M.M. Hannan et al.,2007in vitro clonal propagation of a newly introduced sweetener plant (Stevia rebaudiana Bertoni.) in Bangladesh.]]>2121125Loc, N.H., D.T. Duc, T.H. Kwon and M.S. Yang,2005Curcum zedoaria Roscoe) a valuable medicinal plant.]]>81119122Buchmann, S.L. and M.D. Buchmann,1981Mouriri myrtilloides (Melastomataceae: Memecyleae), an oil flower in Panama.]]>1724Karthikeyan, K., C. Chandran and S. Kulothungan,2009in vitro axillary shoot proliferation of Centella asiatica L.]]>8232235Chandra, I. and P. Bhanja,2002in vitro from callus tissue of Flacourtia jangomas (Lour.) Raeusch through scanning electron microscopy.]]>83476479Anis, M., M. Faisal and S.K. Singh,2003Morus alba L.) through in vitro culture of shoot tip and nodal explants.]]>134751Thengane, S.R., S.V. Bhosle, S.R. Deodhar, K.D. Pawar and D.K. Kulkarni,2006Calophyllum inophyllum), a source of anti-HIV compounds.]]>9013931397