Asian Journal of Biotechnology1996-0700xxxx-xxxxKnowledgia Review10.3923/ajbkr.2009.171.179Escherichia coli iAF1260 Metabolic Network]]>SeyedzadeF. AttarH. 4200914In recent years constraint based methods such as Flux
Balance Analysis (FBA) has widely applied for computation of flux distributions
in the metabolic networks. In this study, the effects of changes in the
intercellular concentration on the Gibbs free energy of the system and
subsequently on the model’s fluxes have studied. This method which
makes a correlation between the flux directions and metabolite concentrations,
has applied to large scale metabolic network, Escherichia coli iAF1260.
The biomass and succinate fluxes have selected as objective functions
and the multi objective genetic algorithm has used to optimization of
the E. coli iAF1260 network. The obtained results revealed that
a living system such as E. coli and its mutants are not stable
from thermodynamic point of view before reaching to the cellular threshold
concentrations. Also the behavior of the mutants of microorganism involving
their return to the wild type phenotypes could be justified.]]>Lee, T.I., N.J. Rinaldi, F. Robert, D.T. Odom and Z. Bar-Joseph et al.,2002298799804Salgado, H., S. Gama-Castro, A. Martinez-Antonio, E. Diaz-Peredo and F. Sanchez-Solano et al.,2004Escherichia coli K-12.]]>32D303D306Shen-Orr, S.S., R. Milo, S. Mangan and U. Alon,2002Escherichia coli.]]>316468Guelzim, N., S. Bottani, P. Bourgine and F. Kepes,2002316063Natalie, C.D., M.J. Herrgard and B.O. Palsson,20041412981309Varma, A., B. Boesch and B. Palsson,1993Escherichia coli glucose catabolism under various oxygenation rates.]]>5924652473Varma, A. and B. Palsson,1993Escherichia coli optimal-growth patterns.]]>165503522Edwards, J., R. Ibarra and B. Palsson,2001Escherichia coli metabolic capabilities are consistent with experimental data.]]>19125130Dien, S.V. and M.E. Lidstrom,200278296312Kayser, A., J. Weber, V. Hecht and U. Rinas,2005Escherichia coli in glucose-limited continuous culture.]]>151693706Covert, M.W., E.M. Knight, J.L. Reed, M.J. Herrgard and B.O. Palsson,20044299296Price, N.D., J.L. Reed and B.O. Palsson,20042886897Palsson, B.O.,20001811471150Pirt, S.J.,1965163224231Henry, C.S., L.J. Broadbelt and V. Hatzimanikatis,20079217921805Kummel, A., S. Panke and M. Heinemann,200623434Henry, C., M. Jankowski, L. Broadbelt and V. Hatzimanikatis,2006Escherichia coli metabolism.]]>9014531461Covert, M.W., I. Famili and B.O. Palsson,200384763772Price, N.D., J.A. Papin, C.H. Schilling and B.O. Palsson,200321162169Reed, J.L. and B.O. Palsson,2003Escherichia coli.]]>18526922699Almaas, E., B. Kovacs, T. Vicsek, Z.N. Oltvai, and A.L. Barabasi,2004Escherichia coli.]]>427839843Edwards, J.S. and B.O. Palsson,2000Escherichia coli MG1655 in silico metabolic genotype: Its definition, characteristics, and capabilities.]]>9755285533Flores, N., J. Xiao, A. Berry, F. Bolivar and F. Valle,1996Escherichia coli.]]>14620623Covert, M.W. and B.O. Palsson,2002Escherichia coli.]]>2772805828064Emmerling, M., M. Dauner, A. Ponti, J. Fiaux and M. Hochuli et al.,2002Escherichia coli.]]>184152164Stols, L. and M.I. Donnelly,1997Escherichia coli mutant.]]>6326952701Varma, A. and B.O. Palsson,1994Escherichia coli W3110.]]>6037243731Kummel, A., S. Panke and M. Heinemann,20067512512Maskow, T. and U. von Stockar,200592223230Mavrovouniotis, M.,19903610701082Mavrovouniotis, M.,19912661444014445Datta, R.,19921992Feist, A.M., C.S. Henry, J.L. Reed, M. Krummenacker and A.R. Joyce et al.,2007Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information.]]>3121121