Asian Journal of Biochemistry1815-99231815-9931Academic Journals Inc.10.3923/ajb.2017.85.90Swietenia macrophylla King Seed on Oxidized LDL, HOMA Beta and Glucagon like Peptide 1 (GLP-1) Gene Expression in Type 2 Diabetic Rats]]>Prasetyastuti Sunarti SadewaAhmad Hamim MursitiSri Mustofa 32017123Background and Objective: Type 2 diabetes is characterized by hyperglycemia leads to increased production of reactive oxygen species. There is increasing evidence that active compounds of medicinal plants may be used to reduce oxidative stress. The aims of this study were to investigate anti-diabetic effects of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one derived from Swietenia macrophylla King seed on oxidized LDL, homeostatic model assessment beta-cell function (HOMA-β) and glucagon like peptide 1 (GLP-1) gene expression in diabetic rats. Materials and Methods: A total of 30 rats were used. They were divided into 6 groups as follows: (A) Normal rats, (B) Diabetic rats, (C) Diabetic rats with metformin, (D), (E) and (F) Diabetic rats with 7-Hydroxy-2-(4-hydroxy-3-methoxy-phenyl)-chromen-4-one Swietenia macrophylla King seed dose 10, 30 or 90 mg/200 g b.wt., respectively. Oxidized LDL and HOMA-β value were analyzed twice, before and after treatment. The GLP-1 gene expression in intestine was analyzed at the end of study. Data were analyzed by paired t-test and one-way ANOVA followed by Games-Howell test. Results: Administration of three different doses of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one from Swietenia macrophylla King seeds in diabetic rats significantly reduced oxidized LDL and increased HOMA-β value (p<0.001) and also GLP-1 gene expression (p<0.05). Conclusion: These findings demonstrate that administration of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one from Swietenia macrophylla King seeds, improved beta cell function through reducing oxidized LDL and increased GLP-1 expression.]]>Tiwari, B.K., K.B. Pandey, A.B. Abidi and S.I. Rizvi,20132013Asmat, U., K. Abad and K. Ismail,201624547553Rahal, A., A. Kumar, V. Singh, B. Yadav, R. Tiwari, S. Chakraborty and K. Dhama,20142014Garrido-Sanchez, L., J.M. Garcia-Almeida, S. Garcoia-Serrano, I. Cardona and J. Garcia-Arnes et al.,20083122582264Ganjifrockwala, F., J. Joseph and G. George,20162016Lee, D.H.,201438274277Abderrahmani, A., G. Niederhauser, D. Favre, S. Abdelli and M. Ferdaoussi et al.,20075013041314Cantley, J. and F.M. Ashcroft,20152015Puddu, A., R. Sanguineti, A. Durante, A. Nencioni, F. Mach, F. Montecucco and G.L. Viviani,20132013Freeman, J.S.,200976S12S19Chang, C.L., Y. Lin, A.P. Bartolome, Y.C. Chen, S.C. Chiu and W.C. Yang,20132013Buteau, J.,200834S73S77Ruhe, R.C. and R.B. McDonald,200120363S369SKhan, A.N., R.A. Khan, M. Ahmad and N. Mushtaq,20153217220Brewer, M.S.,201110221247Maiti, A., S. Dewanjee, G. Jana and S.C. Mandal,2008Swietenia macrophylla seeds against type II diabetes.]]>2224227Hajra, S., A. Mehta, P. Pandey and S.P. Vyas,2011Swietenia mahagoni (Linn.) seeds.]]>3180186Kalaivanan, K. and K.V. Pugalendi,2011Swietenia macrophylla on streptozotocin-diabetic rats.]]>36771Mursiti, S.,2008Swietenia macrophylla King).]]>2008Song, Y., J.E. Manson, L. Tinker, B.V. Howard and L.H. Kuller et al.,20073017471752Masiello, P., C. Broca, R. Gross, M. Roye and M. Manteghetti et al.,199847224229Zaman, A.G., G. Helft, S.G.Worthley and J.J. Badimon,2000149251266Kaplan, M., M. Aviram and T. Hayek,20142014pp: 187-202pp: 187-202Nijveldt, R.J., E. van Nood, D.E.C. van Hoorn, P.G. Boelens, K. van Norren and P.A.M. van Leeuwen,200174418425Coskun, O., M. Kanter, A. Korkmaz and S. Oter,200551117123Kaneto, H., G. Xu, K.H. Song, K. Suzuma, S. Bonner-Weir, A. Sharma and G.C. Weir,20012763109931104Drucker, D.J.,20032629292940Kieffer, T.J. and J.F. Habener,199920876913Ban, K., M.H. Noyan-Ashraf, J. Hoefer, S.S Bolz, D.J. Drucker and M. Husain,200811723402350