Asian Journal of Biochemistry1815-99231815-9931Academic Journals Inc.10.3923/ajb.2016.44.52Cinnamomum iners Leaves as an Alternative Therapy for Diabetes]]>MustaffaFazlina HassanZurina AsmawiMohd Zaini 12016111This study was designed to investigate the antidiabetic property of Cinnamomum iners leaves which were used traditionally to combat diabetes. Firstly, the antidiabetic activity of petroleum ether, chloroform, methanol and water extract of C. iners leaves was evaluated. Next, the potential extract which was the methanol extract was fractionated to obtain ethyl acetate, n-butanol, chloroform and aqueous fraction. The active chloroform fraction was subjected to subfractionation process to obtain subfraction 1 (SF 1) and subfraction 2 (SF 2). Cinnamomum iners leaves active portion (methanol extract, chloroform fraction and subfraction 1) was standardized using cinnamic aldehyde. The antidiabetic activity of standardized C. iners leaves was assessed by using Intra-Peritoneal Glucose Tolerance Test (IPGTT). The antidiabetic mechanism of active portion of C. iners leaves were also determined by measuring glucose absorption from the intestine. This study showed significant glucose tolerance effect of active portion of C. iners leaf after repeated oral administration in STZ-induced diabetic rats. The antidiabetic mechanism of subfraction 1 and chloroform fraction of C. iners leaf is by inhibiting glucose absorption from intestine.]]>Adoum, O.A., H.P. Nenge and C. Bashir,2012Mitragyna inermis (Wild) O. Kundze (Rubiaceae) in alloxan induced diabetic wistar rats.]]>3169174Alamin, M.A., A.I. Yagi and S.M. Yagi,20155395402Burke, J.P., K. Williams, K.M.V. Narayan, C. Leibson, S.M. Haffner and M.P. Stern,20032619992004Chooi, O.H., 20041st Edn.,pp: 132-133pp: 132-133Gokhale, M.S., D.H. Shah, Z. Hakim, D.D. Santani and R.K. Goyal,199837455459Hansotia, T. and D.J. Drucker,2005128125134Hassan, Z., M.F. Yam, M. Ahmad and A.P.M. Yusof,20101590089023Mafauzy, M.,20052005pp: 1011Mustaffa, F., J. Indurkar, S. Ismail, M.N. Mordi, S. Ramanathan and S.M. Mansor,2010Cinnomomum iners leaves methanolic extract.]]>27681Mustaffa, F., J. Indurkar, S. Ismail, M.N. Mordi, S. Ramanathan and S.M. Mansor,2010Cinnomomum iners standardized leaves methanolic extract.]]>6888895Mustaffa, F., J. Indurkar, S. Ismail, M. Shah and S.M. Mansor,2011Cinnamomum iners Leaves with activity against methicillin-resistant Staphylococcus aureus]]>1630373047Nair, S.S., V. Kavrekar and A. Mishra,2013In vitro studies on α amylase and α glucosidase inhibitory activities of selected plant extracts.]]>3128132Pang, K.L., W.L. Thong and S.E. How,2009Cinnamomum iners as mitogen-activated protein kinase kinase (MKK1) inhibitor.]]>1310313Sancheti, S., S. Sancheti, S.H. Lee, J.E. Lee and S.Y. Seo,201110261264Stanely, P., M. Prince and V.P. Menon,2000Tinospora cordifolia roots in alloxan-induced diabetic rats.]]>70915Suba, V., T. Murugesan, G. Arunachalam, S.C. Mandal and B.P. Saha,2004Barleria lupulina extract in rats.]]>11202205Vijayakumar, M.V., S. Singh, R.R. Chhipa and M.K. Bhat,20051464148WHO.,1991ICH Harmonised Tripartite Guideline,19961996pp: 117