Asian Journal of Biochemistry1815-99231815-9931Academic Journals Inc.10.3923/ajb.2011.426.438El-KaderM.A. Abd AliM.M. El-SammadN.M. El-ShaerM.A. 6201166Non steroidal anti-inflammatory drugs are widely used and effective against inflammatory diseases. However, its clinical use is limited by its ulcerogenic effect. This study aimed to investigate the possible gastroprotective effect of alpha-lipoic acid on indomethacin-induced gastric ulcer in rats. Forty adult male Dawley rats were assigned into five groups of eight animals each : group 1 served as control, group 2 was treated intraperitioneally (i.p.) with alpha-lipoic acid (20 mg kg-1 b.w./day) for 7 successive days, group 3: Rats were treated i.p. with indomethacin (20 mg kg-1 b.w./day) for two days, group 4 were treated with indomethacin for 2 days followed by alpha-lipoic acid for 7 days, group 5: Animals were treated with alpha-lipoic acid for 3 days followed by indomethacin for 2 days then alpha-lipoic acid for other 4 days. Alteration of gastric mucosa were analysis. Gastric secretion volume, pH and gastric mucus secretion were determined. In addition, lipid peroxidation and the changes in the antioxidants: reduced glutathione, catalase and superoxide dismutase as markers for antioxidant activity and mucosal defense factors were evaluated. Administration of indomethacin per os induced gastric damage as evidenced by observed ulcers, histological changes, increased gastric secretion volume, decreased pH value and mucus secretion. Alpha lipoic acid were recovered rats from gastric mucosal lesions caused by indomethacin and protected the gastric mucosa covered the stomach wall from injury through decreasing gastric secretion volume, increasing pH value and mucus secretion. All parameters indicating the oxidative injury in gastric mucosa markedly reversed by alpha-lipoic acid treatment due to its potent antioxidant properties where it reduced the level of lipid peroxidation product malondialdehyde, alleviated increases in the catalase activity and ameliorated depressions in reduced glutathione level and the activity of superoxide dismutase caused by indomethacin injection. The results indicate that beneficial effect of alpha-lipoic acid in the protection and treatment of gastric ulcer which result from toxic effect of reactive oxygen species induced by indomethacin.]]>Aebi, H.,1984in vitro.]]>1984pp: 121-126pp: 121-126Ajeigbe, K.O., E.O. Nwobodo, T.O. Oyesola, D.A. Ofusori and S.B. Olaleye,20084482486Al-Attar, A.M.,2011Avicennia alba leaves extract on gastric mucosal damage induced by ethanol.]]>5477490Alsaif, M.A.,20044102109Bandyopadhyay, D., K. Biswas, U. Bandyopadhyay, R.J. Reiter and R.K. Banerjee,200029143151Basivireddy, J., M. Jacob, P. Ramamoorthy, A.B. Pulimood and K.A. Balasubramanian,200365683695Bayir, Y., F. Odabasoglu, A. Cakir, A. Aslan, H. Svleyman, M. Halici and C. Kazaz,200613584590Biewenga, G.P., G.R.M.M. Haenen and A. Bast,199729315331Bilici, M., C. Ozturk, H. Dursun, F. Albayrak and M.B. Saglam et al.,20095418681875Bilska, A. and L. Wlodek,200557570577Chng, H.T., L.S. New, A.H. Neo, C.W. Goh, E.R. Browne and E.C.Y. Chan,200912518086Cicchillo, R.M., D.F. Iwig, A.D. Jones, N.M. Nesbitt and C. Baleanu-Gogonea et al.,20044363786386Copeman, M., J. Matuz, A.J. Leonard, J.P. Pearson, P.W. Dettmar and A. Allen,19942, carbolpolyacrylate, sucrafate and bismuth salicylate.]]>95559Corne, S.J., S.M. Morrissey and R.J. Woods,1974242116P117PDengiz, G.O., F. Odabasoglu, Z. Halici, H. Suleyman, E. Cadirci and Y. Bayir,20073014261434Draper, H.H. and M. Hadley,1990186421431Durak, I., O. Canbolat, M. Kavutcu, H.S. Ozturk and Z. Yurtaslani,1996101720Demircan, B., G. Celik, H. Suleyman and F. Akay,200517383388Ellman, G.L.,1959827077El-Missiry, A.M., H.I. El-Sayed and I.A. Othman,200138694700Gill, N.S., J. Bajwa, P. Sharma, K. Dhiman and S. Sood et al.,2011Cucumis melo seeds.]]>68289Goel, R.K. and S.K. Bhattacharya,199129701714Grant, H.W., K.R. Palmer, R.W. Kelley, N.H. Wislon and J.J. Misewicz,198894955959Guidobono, F., F. Pagani, C. Ticozzi, V. Sibilia, A. Pecile and C. Netti,1997120581586Gul, M., F..Z. Kutay, S. Temocin and O. Hanninen,200038625634Huang, J.Q., S. Sridhar and R.H. Hunt,2002Helicobacter pylori infection and non-steroidal anti-inflammatory drugs in peptic-ulcer disease: A meta-analysis.]]>3591422Jiang, G.L., W.B. Im, Y. Donde and L.A. Wheeler,20091551495156Leonard, A., M.T. Droy-Lefaix and A. Allen,19942.]]>18609616Maritim, A.C., R.A. Sanders and J.B. Watkins,200314288294Marnett, L.J. and C.L. Wilcox,1977487222230Naito, Y., T. Yoshikawa, N. Yoshida and M. Kondo,19984330S34SOdabasoglu, F., Z. Halici, A. Cakir, M. Halici and H. Aygun et al.,2008591300306Ogle, C.W., C.H. Cho, M.C. Tong and M.W.L. Koo,1985112399404Olaleye, S.B. and K.O. Ajeigbe,200998792Oluwole, F.S. and M.T. Saka,20077678681Packer, L., E.H. Witt and H.J. Tritschler,199519227250Repetto, M.G. and S.F. Llesuy,200235523534Ross, M.H., E.J. Reith and L.J. Romrell,19892nd Edn.,Pages: 783Pages: 783Shay, P.K., R.F. Moreau, E.J. Smith and T.M. Hagen,200860362367Smith, W.L. and L.J. Marnett,19911083117Song, K.H., W.J. Lee, J.M. Koh, H.S. Kim and J.Y. Youn et al.,2004326197202Suh, J.H., H. Wang, R.M. Liu, J. Liu and T.M. Hagen,2004423126135Suh, J.H., R. Moreau, S.H. Heath and T.M. Hagen,2005105260George, S., A. Sathiamoorthy and S.S. Sathimoorthy,199931431433Takeuchi, K., A. Tanaka, Y. Hayashi and A. Yokota,20055475486Winiarska, K., D. Malinska, K. Szymanski, M. Dudziak and J. Bryla,200890450459Rifat-uz-Zaman, M.S. Akhtar and M.S. Khan,2004Nigella sativa seed and its extracts in indomethacin-treated rats.]]>79951000Rifat-uz-Zaman, M.S. Akhtar and M.S. Khan,2005Polygonum viviparum L. root and its extracts against lipid-peroxidation induced by Indomethacin in rats.]]>1324328