Asian Journal of Biochemistry1815-99231815-9931Academic Journals Inc.10.3923/ajb.2011.347.356GuptaAnkush SinghSangram JamalFarrukh NathSom MehrotraSudhir SharmaBechan 4201164In this study, the potential synergistic relationship between reduced glutathione and vitamin E on ethanol toxicity in isolated rat hepatocytes were examined. It was observed that treatment of ethanol (5-25 mM) caused decrease in cell viability but in presence of Rotenone, cell viability decreased to a greater extent. Inhibitory concentration (IC50) was found to be 20 mM of ethanol treated with rotenone (10 μM) for 2 h. Treatment of ethanol with rotenone (20 mM±10 μM) caused increase in lipid peroxidation, increase of lactate dehydrogenase and decrease in reduced glutathione content. Reduced glutathione and α-tocopherol, alone and in combination, were added to hepatocyte suspension in an attempt to protect cells against alcohol induced cell damage. Pretreatment with reduced glutathione and vitamin E independently, increased the cell viability to 78.97 and 70.42%, respectively, but a combination of reduced glutathione and vitamin E increased cell viability to 92.12%. Vitamin E reduced lipid peroxidation by 41.32% but in combination with reduced glutathione decreased lipid peroxidation by 61.15%. The results suggest an interaction between GSH and Vitamin E in protecting ethanol induced hepatocytes damage.]]>Abdel-Hamid, N.M., L.M. Faddah, M.A. Al-Rehany and A.A. Awad,200833237Adachi, M., H. Higuchi, S. Miura, T. Azuma and S. Inokuchi et al.,2004287695705Adachi, M. and H. Ishii,200232487491Bailey, S.M., E.C. Pietsch and C.C. Cunningham,199927891900Bansal, A.K., M. Bansal, G. Soni and D. Bhatnagar,2005N-nitrosodiethylamine induced oxidative stress in rat liver.]]>156101111Brand, M.D., C. Affourtit, T.C. Esteves, K. Green and A.J. Lambert et al.,200437755767Caetano, R., P.A.C. Vaeth, S. Ramisetty-Mikler and L.A. Rodriguez,200933150159Cadenas, E. and K.J.A. Davies,200029222230Cunningham, C.C., W.B. Coleman and P.I. Spach,199025127136Draper, H.H. and M. Hadley,1990186421431Duke, R.C., D.M. Ojcius and J.D.E. Young,19962757987Griffith, O.W.,1980106207212Guerri, C. and S. Grisolia,1980126365384Hao, W., Z. Su, B. Liu, K. Zhang and H. Yang et al.,2004394352Higuchi, H., M. Adachi, S. Miura, G.J. Gores and H. Ishii,200134320328Jordao, Jr., A.A., P.G. Chiarello, M.R. Arantes, M.S. Meirelles and H. Vannucchi,200442459464Koch, O., S. Farre, M.E De Leo, P. Palozza and B. Palazzotti et al.,200035159163Kypri, K., M.J. Paschall, J. Langley, J. Baxter, M. Cashell-Smith and B. Bourdeau,200933307314Leedle, R.A. and S.D. Aust,199025241245Li, J., B.A. French, P. Fu, F. Bardag-Gorce and S.W. French,2003285442448Li, J., B.A. French, P. Fu, F. Bardag-Gorce and S.W. French,20047530433051Machlin, L.J.,1980pp: 660pp: 660Mahmoud, A.H.,2006Urtica pilulifera extracts.]]>1189196Mahmoud, A.H., H.M. Motawa, H.E. Wahba and A.Y. Ebrahim,2006Urtica pilulifera extracts.]]>16774Marjani, A., A. Moradi and A.B. Ghourcaie,20072118123Marks, D.B., A.D. Marks and C.M. Smith,1996pp: 327-340pp: 327-340Morel, Y. and R. Barouki,1999342481496Molina-Jimenez, M.F., M.I. Sanchez-Reus, D. Andres, M. Cascales and J. Benedi,20041009916Mosmann, T.,1983655563Nagy, L.E.,2004245578Ray, D.E.,19911991pp: 585-636pp: 585-636Sakka, N., H. Sawada, Y. Izumi, T. Kume and H. Katsuki et al.,20031424252428Seglen, P.O.,1976132983Sherer, T.B., R. Betarbet, C.M. Testa, B.B. Seo and J.R. Richardson et al.,2003231075610764Slater, T.F.,1984222115Slater, A.F., C. Stefan, I. Nobel, D.J. van den Dobbelsteen and S. Orrenius,199582/83149153Sridevi, M., G. Chandramohan and K.V. Pugalendi,2007Solanum surattense Leaf-extract on blood glucose, oxidative stress and hepatic marker enzymes in STZ-diabetic rats.]]>2247255Sun, A.Y., M. Ingelman-Sundberg, N. Neve, H. Matsumoto, Y. Nishitani and Y. Minowa,200125237243Turkdogan, M.K., Z. Agaoglu, Z. Yener, R. Sekeroglu, H.A. Akkan and M.E. Avci,2001Nigella sativa in the prevention of liver fibrosis and cirrhosis in rabbits: New hopes.]]>1087173Turrens, J.F.,2003552335344Turrens, J.F. and A. Boveris,1980191421427Wallin, B., B. Rosengren, H.G. Shertzer and G. Camejo,19932081015Yu, B.P.,199474139162Fukami, J., T. Shishido, K. Fukunaga and J.E. Casida,19961712171226