Asian Journal of Biochemistry1815-99231815-9931Academic Journals Inc.10.3923/ajb.2010.33.39Hevea brasiliensis) Seed Oil as
Rats and Shrimps Tests]]>AbdullahBashar Mudhaffar SalimonJumat 1201051The lipid fraction of Malaysian rubber seed was extracted and analyzed for toxicological compounds such as linamarin. Analytical methods (rats toxicological test and brine shrimps test) were applied to determine the presence of such compounds. Malaysian rubber seed oil was extracted using different solvents (hexane and chloroform+methanol). The oil was found not to contain unusual fatty acids and it is rich in essential unsaturated fatty acids, particularly linoleic (37.28±0.10%) and linolenic (19.22±0.21%). Malaysian rubber seed oil showed no toxic potential toward the rats. Bioassay experiments, in which shrimps were used as test organisms, were performed to evaluate the toxicity of linamarin extracted from the oil. The median lethal concentration (LC50) was found to be 211.70%. The results showed no acute toxicity effects, probably since Malaysian rubber seed oil was not found to contain any hazardous linamarin.]]>Achinewhu, S.C. and M.A. Akpapunam,1985Hevea brasiliensis) and bread-fruit (Artocarpus altilis).]]>35103107Aigbodion, A.I. and I.O. Bakare,200582465469ASTM,1998Bjarnholt, N., M. Laegdsmand, H.C.B. Hansen, O.H. Jacobsen and B.L. Mollar,200872897904Duke, J.A. and J.L. Ducellier,1993Dzombak, D.A., R.S. Ghosh and G.M. Wong-Chong,2006pp: 602pp: 602Ghandhi, V.M., K.M. Cherian and M.J. Mulky,199067883886Gunstone, F.D., J.L. Harwood and F.B. Padley,19942nd Edn.,Idibie, C.A., H. Davids and S.E. Iyuke,200730261269Nwokolo, E., D.D. Kitts and J. Kanhai,198838145153OECD,1993Onyeike, E.N. and G.N. Acheru,200277431437Siritunga, D. and R. Sayre,2004Manihot esculenta).]]>56661669Sornyotha, S., K. Laykyu and C.K. Ratanakhanok,200610417501754US EPA.,199950 Software Program.]]>Version 1.00.Yong, O.Y. and S. Jumat,2006Elateriospermum tapos seed oil as a new source of oilseed.]]>24146151APHA.,199820th Edn.,Pages: 1220Pages: 1220SAS.,1996Version 5,