Asian Journal of Biochemistry1815-99231815-9931Academic Journals Inc.10.3923/ajb.2008.11.18RamachandranS. PrasadN. Rajendra PugalendiK.V. P. MenonVenugopal 1200831UV radiation-induced damages may result in pre-cancerous and cancerous
lesions and acceleration of skin aging. It involves an imbalance of the
endogenous antioxidant system that leads to the increase of free radical
levels. Antioxidant pretreatment might inhibit such imbalance. In the
present study, the photoprotective effect of ursolic acid (UA; 3β-hydroxy-urs-12-en-28-oic
acid), a dietary polyphenolic phytochemical, has been examined in the
UVB-(280-320 nm) irradiated human blood lymphocytes. Lymphocytes pretreated
with increasing concentrations of ursolic acid (1, 5 and 10 μg mL-1)
for 30 min, were irradiated and lipid peroxidation and antioxidant defense
were examined. UVB-irradiated lymphocytes exhibited increased levels of
lipid peroxidation and disturbances in antioxidant status. Ursolic acid
pretreatment resulted in significant reduction in thiobarbituric acid
reactive substances (TBARS) and lipid hydroperoxides (LPH) levels. Further,
antioxidants like superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GPx), reduced glutathione (GSH), vitamin-C (Vit-C) and vitamin-E
(Vit-E) were normalised in ursolic acid pretreated plus UVB-treated lymphocytes.
The maximum dose of ursolic acid (10 μg mL-1) normalized
the UVB induced lipid peroxidation, indicating the photoprotective effect
of ursolic acid in human peripheral lymphocytes under in vitro
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