Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2018.360.368Ovis aries) under Post-parturient and High Protein Diet Conditions]]>FarmanMohamad TripathiShiv Kumar TejNikhil Kumar NandiSumanta GuptaPaluru Subramanyam Parameswara MondalSukanta VenkateshGirish Kumar 42018134Background and Objective: Metabolic stress negatively impairs animal health and production. Investigating the changes in the behavioural and hemato-biochemical profile of metabolic stressed animals could serve as clinical markers of metabolic stress. Identification of such indicators could lead to the improvement of treatment success in metabolic disorders. The present study aimed to investigate the indicators of metabolic stress under post-parturient and high protein diet conditions in ewes via changes in behavioural, haematological, serum metabolites, urinary metabolites, serum and urine cortisol, metabolic hormones and electrolyte and enzymatic parameters. Materials and Methods: One hundred and twenty female cycling ewes of 2.5-3 years of age were selected and assigned to three groups, group-I: Control (n = 40), group-IIa: Post-parturient (n = 40) and group-IIb: High protein diet (n = 40). Whole blood was collected and used for haematological assay. Serum was used for estimation of biochemical, enzymatic, hormonal and electrolyte analysis. Results: Higher concentrations of Non-esterified fatty acids (NEFA), β-hydroxybutyric acid(β-OHB), cortisol and calcium in the serum of post-parturient ewes (group-IIa) compared to high protein diet (group-IIb) and control ewes (group-I). Changes in concentrations of biochemical parameters (glucose, cholesterol, total NEFA, β-OHB, T3, T4, insulin growth factor-1 (IGF1) insulin, calcium, magnesium, alkaline phosphatase and lactic dehydrogenase) indicated an energy insufficiency of ewes in metabolic stressed ewes (Group-II). Increase of White Blood Cells (WBC) count, hemoglobin and haematocrit values and behavioural changes were also observed in post-parturient ewes (group-IIa). Conclusion: Metabolic stress has a significant influence on serum levels of glucose, urea, ammonia and β-OHB, stress related hormones, calcium, magnesium, Alanine Aminotransferase (ALT) and Lactate Dehydrogenase (LDH).]]>Sejian, V., S.M.K. Naqvi, T. Ezeji, J. Lakritz and R. Lal,2012Farman, M., S.K. Tripathi, S. Nandi and V.G. Kumar,20159466470Khodaei-Motlagh, M., A.Z. Shahneh, R. Masoumi and F. Derensis,20111055525558Kor, N.M., K.M. Khanghah and A. Veisi,20133397404Tripathi, S.K., M. Farman, S. Nandi, V.G. Kumar and P.S.P. Gupta,2016Ovis aries).]]>144225228Hardarson, G.H.,2002956367Cal-Pereyra, L., A. Benech, J.R. Gonzalez-Montana, J. Acosta-Dibarrat, S. Da Silva and A. Martin,201563141146Saco, Y., M. Fina, M. Gimenez, R. Pato, J. Piedrafita and A. Bassols,2008177439441Chapinal, N., M. Carson, T.F. Duffield, M. Capel and S. Godden et al.,20119448974903Esposito, G., P.C. Irons, E.C. Webb and A. Chapwanya,20141446071Sepulveda-Varas, P., D.M. Weary, M. Noro and M.A. von Keyserlingk,20152015Antunovic, Z., J. Novoselec, H. Sauerwein, M. Speranda, M. Vegara and V. Pavic,201117687695Tej, J.N.K., G.G. Varma, M. Abhina, N. Adarsh, K. Karthiayini and V.L. Gleeja,20172017Tripathi, S.K., M. Farman, S. Nandi, S. Mondal, P.S.P. Gupta and V.G. Kumar,2016In vitro culture of oocytes and granulosa cells collected from normal, obese, emaciated and metabolically stressed ewes.]]>1708389OECD.,19981998Fazio, E., P. Medica, S. Mignacca, C. Cravana and A. Ferlazzo,20111024872492Guesdon, V., S. Ligout, P. Delagrange, M. Spedding and F. Levy et al.,2012105203208Guesdon, V., B. Malpaux, P. Delagrange, M. Spedding and F. Cornilleau et a.,20133814261434Nandi, S., S. Mondal, D.T. Pal and P.S.P. Gupta,2015100309315Mohammadi, V., E. Anassori and S. Jafari,201673539Roubies, N., N. Panousis, A. Fytianou, P.D. Katsoulos, N. Giadinis and H. Karatzias,200653277281Eski, F., I. Tasal, M.A. Karsli, S. Sendag, B.A. Uslu, H. Wagner and A. Wehrend,201539401405Fiore, E., G. Piccione, M. Gianesella, V. Pratico, I. Vazzana, S. Dara and M. Morgante,201558403406Flier, J.S., M. Harris and A.N. Hollenberg,2000105859861Novoselec, J., Z. Antunovic, M. Speranda, Z. Steiner and T. Speranda,20098208210Garcia-Garcia, R.M.,20122012Deghnouche, K., M. Tlidjane, T. Meziane and A. Touabti,2013819201924Rana, M.S., M.A. Hashem, M.N. Sakib and A. Kumar,2014129194Sivakumar, A.V.N., G. Singh and V.P. Varshney,20102314621468Alves, E.M., D.R. Magalhaes, M.A. Freitas, E.D.J.D. Santos, M.L.A. Pereira and M.D.S. Pedreira,2014365562Mohammed, A.L.,200987177Caroprese, M., M. Albenzio, A. Marzano, L. Schena, G. Annicchiarico and A. Sevi,20109323952403Verbeek, E., M.H. Oliver, J.R. Waas, L.M. McLeay, D. Blache and L.R. Matthews,20122012Ali, S., M.K. Awasthi, J.R. Khan, R.P. Tiwari and M.R. Poyam,2015332528Urdl, M., L. Gruber, W. Obritzhauser and A. Schauer,201599174189