Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2017.302.310Listeria monocytogenes and the Extent of its Resistance to Different Disinfectants in Dairy Farm for Improving Animal Health]]>MohammedAsmaa Nady Abdel AzizSahar Abdel Aleem 62017126Background and Objective: Resistance of pathogenic bacteria to disinfection process represent a potential threat to the animal health and food industry in developing countries. This study designed to monitor the main source of Listeria monocytogenes (L. monocytogenes) in animal's environment and assess the resistance pattern of Listeria monocytogenes to different disinfectants used for improving animal health and product quality. Materials and Methods: Animal, human and environmental samples, (n = 80, 40, 200, respectively) include [milk, animal feces, human stool, air dust, water, biofilm, bulk tank milk swabs, feedstuff and soil] are collected from a dairy cattle farm. Samples were cultured for isolation and identification of Listeria monocytogenes using biochemical tests and PCR. Sensitivity test of Listeria monocytogenes to different disinfectants [benzalkonium chloride (BC), hydrogen peroxide (H2O2), Virkon®S, TH4+ and sodium hypochlorite] was evaluated using broth macrodilution method. Resistant bacteria to disinfectants were screened for the presence of resistant gene using PCR. Data was analyzed by Chi square test. Results: The highest percentage of L. monocytogenes was isolated from soil, milk equipment, biofilm and feedstuff (45.0, 42.5, 32.5 and 25.0%, respectively). Listeria monocytogenes was moderately resistant against benzalkonium chloride1000 mg L1, Virkon®S 2%, TH4+ 1%, H2O2 5% and sodium hypochlorite 500 mg L1 (50, 40, 50, 50, 60, 40, 20, 30, 30 and 40%, respectively) at both 12 and 24 h of exposure times. Furthermore, resistance qacED1 gene was found in L. monocytogenes isolates. Conclusion:Listeria monocytogenes was highly resistant against all tested disinfectants at both 4 and 6 h of exposure times. Animal feces, soil and milk equipment serve as the main reservoir of L. monocytogenes in a dairy farm. Regular monitoring of microbial sensitivity to disinfectants used and improving the disinfectants power have an essential role for controlling of L. monocytogenes resistant bacteria in dairy cattle farms.]]>Locatelli, A., A. Spor, C. Jolivet, P. Piveteau and A. Hartmann,2013Listeria monocytogenes in soil.]]>2013De Noordhout, C.M., B. Devleesschauwer, F.J. Angulo, G. Verbeke and J. Haagsma et al.,20141410731082Rodrigues, C.S., C.V.G.C. de Sa and C.B. de Melo,2017Listeria monocytogenes contamination in ready to eat meat, dairy and fishery foods.]]>2017Shiwakoti, S.,2015Listeria monocytogenes in the pre-harvest environment; a landscape epidemiology approach.]]>2015Borucki, M.K., C.C. Gay, J. Reynolds, K.L. McElwain, S.H. Kim, D.R. Call and D.P. Knowles,2005Listeria monocytogenes strains from a high-prevalence dairy farm.]]>7158935899Fox, E.M., N. Leonard and K. Jordan,2011Listeria monocytogenes isolates.]]>7765596569Tocmo, R., K. Krizman, W.J. Khoo, L.K. Phua, M. Kim and H.G. Yuk,2014Listeria monocytogenes in vacuum-packed smoked fish products: Occurrence, routes of contamination and potential intervention measures.]]>13172189Gomez, D., L.P. Iguacel, M. Rota, J.J. Carraminana, A. Arino and J. Yanguela,2015Listeria monocytogenes in ready-to-eat meat products and meat processing plants in Spain.]]>4271282Hellstrom, S.,2011Listeria monocytogenes in food production.]]>2011Kastbjerg, V.G. and L. Gram,2009Listeria monocytogenes.]]>10616671681Ibusquiza, P.S., J.J.R. Herrera and M.L. Cabo,2011Listeria monocytogenes.]]>28418425Carballo, J. and A.B. Araujo,2012Listeria monocytogenes and Salmonella spp.]]>32606612Cabeca, T.K., A.C. Pizzolitto and E.L. Pizzolitto,20124311121119Dobeic, M., E. Kenda, J. Micunovic and I. Zdovc,2011Listeria spp. in the red meat processing industry.]]>29441447Nassirabady, N., H. Meghdadi and A. Alami,2015Listeria monocytogenes of Karun river (environmental sources rural and urban) by culture and PCR assay.]]>2015Hitchins, A.D.,2001Listeria monocytogenes.]]>2001Walsh, P.S., D.A. Metzger and R. Higuchi,199110506513Abu Al-Soud, W. and P. Radstrom,19986437483753Deneer, H.G. and I. Boychuk,1991Listeria monocytogenes by DNA amplification.]]>57606609Gilbert, P. and L.E. Moore,200599703715Meyer, B.,2006112275279Li, Q., S. Mahendra, D.Y. Lyon, L. Brunel and M.V. Liga et al.,20084245914602Chuanchuen, R., S. Khemtong and P. Padungtod,2007QACE/QACE∆1 genes and their correlation with class 1 integrons in Salmonella enterica isolates from poultry and swine.]]>38855862Manyi-Loh, C.E., S.N. Mamphweli, E.L. Meyer, G. Makaka, M. Simon and A.I. Okoh,20162016Nightingale, K.K., Y.H. Schukken, C.R. Nightingale, E.D. Fortes and A.J. Ho et al.,2004Listeria monocytogenes infecting ruminants and in the farm environment.]]>7044584467Jiang, X., M. Islam, J. Morgan and M.P. Doyle,2004Listeria monocytogenes in bovine manure-amended soil.]]>6716761681Harvey, J., K.P. Keenan and A. Gilmour,2007Listeria monocytogenes strains.]]>24380392Cesar, A.P.R., A.J. de Mesquita, C.S. Prado, I.A. Nunes and E.S. de Almeida Filho,2011Listeria spp. and Listeria monocytogenes in industrial plants of hot dog Frankfurters].]]>12339352, (In Portuguese)Leong, D., A. Alvarez-Ordonez and K. Jordan,2014Listeria monocytogenes in foods and food processing environments in the Republic of Ireland.]]>2014Tiwari, U., D. Walsh, L. Rivas, K. Jordan and G. Duffy,2014Listeria monocytogenes in raw and pasteurised semi-soft rind washed milk cheese during storage following ripening.]]>42248256Conficoni, D., C. Losasso, E. Cortini, A. Di Cesare and V. Cibin et al.,2016Listeria monocytogenes collected in meat-processing environments.]]>2016Poole, K.,200739162176Pu, Y., Z. Zhao, Y. Li, J. Zou and Q. Ma et al.,201662284294Aase, B., G. Sundheim, S. Langsrud and L.M. Rorvik,2000Listeria monocytogenes.]]>625763Morente, E.O., M.A. Fernandez-Fuentes, M.J.G. Burgos, H. Abriouel, R.P. Pulido and A. Galvez,20131621325Thevenot, D., A. Dernburg and C. Vernozy-Rozand,2006Listeria monocytogenes in the pork meat industry and its products.]]>101717Langsrud, S., M.S. Sidhu, E. Heir and A.L. Holck,200351283290Tamburro, M., G. Ripabelli, M. Vitullo, T.J. Dallman, M. Pontello, C.F.L. Amar and M.L. Sammarco,2015Listeria monocytogenes exposed to sublethal concentration of benzalkonium chloride.]]>403139Hutchison, M.L., L.D. Walters, T. Moore, D.J.I. Thomas and S.M. Avery,200571691696Warnes, S.L., S.M. Green, H.T. Michels and C.W. Keevil,20107653905401Belluco, S., C. Losasso, I. Patuzzi, L. Rigo and D. Conficoni et al.,2016Listeria monocytogenes.]]>2016Zhu, Q., R. Gooneratne and M.A. Hussain,2017Listeria monocytogenes in fresh produce: Outbreaks, prevalence and contamination levels.]]>2017Lemaitre, J.P., H. Echchannaoui, G. Michaut, C. Divies and A. Rousset,19986114591464