Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2017.132.141PadetaIrma NugrohoWidagdo Sri KusindartaDwi Liliek FibriantoYuda Heru BudipitojoTeguh 32017123Background: Mesenchymal stem cell-conditioned medium (MSC-CM) is the substance extracted from mesenchymal stem cell culture,
contains a lot of potential cytokines and growth factors as regenerative agent. Objective: The aim of this study was to investigate the
role of MSC-CM on the burn wound regeneration pattern of white rat (Rattus norvegicus ). Methodology: Rat was anesthetized using
combination of 10% ketamine and 2% xylazine at the dose rate of 75 and 5 mg kg1 b.wt., respectively. The burn wound condition was
created on the dorsal area of each rat. The burn wound area of control group was treated with Bioplacenton®, while MSC-CM group was
topically treated with MSC-CM cream twice a day. The diameter of burn wound was measured every 5 days from wounded. Skin wound
tissues were collected at 4 h, followed by 2, 5, 10, 15, 20, 25 and 30 days after wounded and then were processed by paraffin method.
Tissue samples were visualized by using hematoxylin-eosin and Massons trichrome stain. The polymer-based immunohistochemistry
method was employed to detect the presence of basic fibroblast growth factor (bFGF) as important growth factor in wound healing
process. Result: The results showed that MSC-CM promote the recovery of skin burn wound in white rat, as indicated by: (1) Acceleration
of wound closure, (2) Greater numbers of fibroblasts, (3) High density of collagen fibers and (4) Greater numbers of blood vessels in
MSC-CM group compare with control group. Conclusion: Since the number of bFGF immunoreactive cells increased significantly during
recovery proccess in MSC-CM group compare with control group, it was suggested that bFGF plays important role on the tissues
regeneration of skin burn treated by MSC-CM.]]>Maxson, S., E.A. Lopez, D. Yoo, A. Danilkovitch-Miagkova and M.A. LeRoux,20121142149Han, Y., J. Chai, T. Sun, D. Li and R. Tao,2011in vitro.]]>413561565Pawitan, J.A.,20142014Ghaderi, R., M. Afshar, H. Akhbarie and M.J. Golalipour,201028193198Zhang, C.P. and X.B. Fu,200811209221Ghieh, F., R. Jurjus, A. Ibrahim, A.G. Geagea and H. Daouk et al.,20152015Macri, L. and R.A.F. Clark,2009228393Kondo, T. and Y. Ishida,20102039398Gnecchi, M., Z. Zhang, A. Ni and V.J. Dzau,200810312041219Kibe, Y., H. Takenaka and S. Kishimoto,2000143720727Moya, M.L., M.R. Garfinkel, X. Liu, S. Lucas, E.C. Opara, H.P. Greisler and E.M. Brey,2010160208212Carpenter, J.W., T.Y. Mashima and D.J. Rupiper,20012nd Edn.,Pages: 423Pages: 423Kim, H.O., S.M. Choi and S.H. Kim,20131093101Mirabella, T., M. Cilli, S. Carlone, R. Cancedda and C. Gentili,20113236893699Chen, L., E.E. Tredget, P.Y.G. Wu and Y. Wu,20082008Liu, Y., R. Mu, S. Wang, L. Long and X. Liu et al.,20102010Akita, S., K. Akino and A. Hirano,201324449Gibran, N.S., F.F. Isik, D.M. Heimbach and D. Gordon,199456226234Guo, S. and L.A. Dipietro,201089219229Rivera, A.E. and J.M. Spencer,2007253948Yolanda, M.M., A.V. Maria, F.G. Amaia, P.B. Marcos, P.L. Silvia, E. Dolores and O.H. Jesus,20142014Singer, A.J. and R.A.F. Clark,1999341738746Broughton II, G., J.E. Janis and C.E. Attinger,20061171e-S32e-SFeiken, E., J. Romer, J. Eriksen and L.R. Lund,1995105120123Akita, S., K. Akino, T. Imaizumi and A. Hirano,200816635641Gurtner, G.C., M.J. Callaghan and M.T. Longaker,200758299312Mishra, P.J., P.J. Mishra and D. Banerjee,201243543Giannouli, C.C. and D. Kletsas,20061814171429Chithra, P., G.B. Sajithlal and G. Chandrakasan,19981817176Afshar, M., R. Ghaderi, M. Zardast and P. Delshad,20162016Kim, J.Y., S.H. Song, K.L. Kim, J.J. Ko and J.E. Im et al.,20101916351644Shrestha, C., L. Zhao, K. Chen, H. He and Z. Mo,20132013Shen, C., P. Lie, T. Miao, M. Yu and Q. Lu et al.,2015122030Velnar, T., T. Bailey and V. Smrkolj,20093715281542Greaves, N.S., K.J. Aschroft, M. Baguneid and A. Bayat,201372206217Stadelmann, W.K., A.G. Digenis and G.R. Tobin,199817626S38SAkasaka, Y., I. Ono, T. Yamashita, K. Jimbow and T. Ishii,2004203710720Kusindarta, D.L., H. Wihadmadyatami, Y.H. Fibrianto, W.S. Nugroho and H. Susetya et al.,20169605610Atala, A., D.J. Irvine, M. Moses and S. Shaunak,201035122Guo, N., H.C. Krutzsch, J.K. Inman and D.D. Roberts,19975717351742Fu, X., Z. Shen, Y. Chen, J. Xie, Z. Guo, M. Zhang and Z. Sheng,199835216611664