Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2016.248.252Clostridium
perfringens from Broilers with Necrotic Enteritis]]>MostafaAlaa El-din Hussein Eman El-ShahatAbdeen Abou-HadeedMostafa Gamal 42016114Necrotic Enteritis (NE) is the most common and financially devastating bacterial disease in modern broiler flocks. A previously unknown pore forming toxin, which called new toxin Perfringens necrotic enteritis B-like toxin (NetB toxin) and the encoding gene, netB, which is produced by some Australian strains of Clostridium perfringens has recently been reported. This toxin was reported to be critical for the development of necrotic enteritis in chickens. To investigate the occurrence of the toxin gene (netB) in non-Australian C. perfringens strains, intestinal samples of fifty diseased broilers chickens and fifty apparently healthy broilers in Egypt were examined. The netB gene was found in some isolates while alpha toxin, which encoded by cpα gene was detected in all isolates from chickens. In contrast, none of the isolates carried the enterotoxin gene. The present study indicates the role of netB in the induction of necrotic enteritis in correlation with α-toxin while, no significant effect of enterotoxin of C. perfringens in the occurrence of this disease in broiler chickens.]]>Shojadoost, B., A.R. Vince and J.F. Prescott,2012Clostridium perfringens: A critical review.]]>2012Engstrom, B.E., C. Fermer, A. Lindberg, E. Saarinen, V. Baverud and A. Gunnarsson,2003Clostridium perfringens from healthy and diseased poultry.]]>94225235Johansson, A., A. Aspan, M. Kaldhusdal and B.E. Engstrom,2010netB in Clostridium perfringens isolated from a broiler flock affected by mild necrotic enteritis.]]>1448792Baums, C.G., U. Schotte, G. Amtsberg and R. Goethe,2004Clostridium perfringens isolates.]]>1001116Thomas, P., T.R. Arun, K. Karthik, P.V. Berin and M.A. Kumar et al.,2014Clostridium perfringens isolate from a case of necrotic enteritis in Indian kadaknath fowl.]]>9385394Gholamiandekhordi, A.R., R. Ducatelle, M. Heyndrickx, F. Haesebrouck and F. Van Immerseel,2006Clostridium perfringens isolates from poultry flocks with different disease status.]]>113143152Miyakawa, M.E.F. and F.A. Uzal,2005Clostridium perfringens type A α toxin in the intestine of sheep.]]>66251255Keyburn, A.L., J.D. Boyce, P. Vaz, T.L. Bannam and M.E. Ford et al.,2008Clostridium perfringens.]]>2008Keyburn, A.L., X.X. Yan, T.L. Bannam, F. van Immerseel, J.I. Rood and R.J. Moore,2010Clostridium perfringens strains expressing NetB toxin.]]>2010Timbermont, L., L. de Smet, V.F. Nieuwerburgh, R.V. Parreira and V.G. Driessche et al.,2014netB positive Clostridium perfringens strains from broilers with necrotic enteritis.]]>2014Oakley, C.L. and G.H. Warrack,1953Clostridium welchii.]]>51102107Sterne, M. and I. Batty,1975Pages: 144Pages: 144Komoriya, T., A. Hashimoto, A. Shinozaki, M. Inoue and H. Kohno,2007Clostridium perfringens.]]>2007Van Asten, A.J.A.M., C.W. van der Wiel, G. Nikolaou, D.J. Houwers and A. Grone,2009Clostridium perfringens isolates.]]>136411412Meer, R.R. and J.G. Songer,1997Clostridium perfringens.]]>58702705Sambrook, J. and D.W. Russell,20023rd Edn.,pp: 18-96pp: 18-96Miah, M.S., M. Asaduzzaman, M.A. Sufian and M.M. Hossain,2011Clostridium perfringens, causal agents of necrotic enteritis in chickens.]]>997102Kalender, H. and H.B. Ertas,2005Clostridium perfringens from chickens and detection of the alpha toxin gene by Polymerase Chain Reaction (PCR).]]>29847851Manfreda, G., V. Bondioli, A. de Cesare and A. Franchini,2006Clostridium perfringens in Italian broilers.]]>629192Craven, S.E., N.J. Stern, J.S. Bailey and N.A. Cox,2001Clostridium perfringens in broiler chickens and their environment during production and processing.]]>45887896Osman, K.M., Y.A. Soliman, Z.M. Amin and M.A. Aly,2012Clostridium perfringens type a isolates in commercial broiler chickens and parent broiler breeder hens in Egypt.]]>31931941Shanmugasamy, M. and J. Rajeswar,2012Clostridium perfringens in poultry.]]>5365368Doosti, A., M. Pasand, A. Mokhtari-Farsani, R. Ahmadi, M. Chehelgerdi,2015Clostridium perfringens type A isolates in different tissues of broiler chickens.]]>2015Lepp, D., B. Roxas, V.R. Parreira, P.R. Marri and E.L. Rosey et al.,2010Clostridium perfringens strains that cause avian necrotic enteritis.]]>2010Timbermont, L., F. Haesebrouck, R. Ducatelle and F. van Immerseel,201140341347Bailey, M.A., K.S. Macklin and J.T. Krehling,2015netB and tpeL in historical Clostridium perfringens isolates from broiler farms in Alabama.]]>594651