Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2015.271.280AshmawyNeama A.62015106The aim of this study is to determine the influence of physiological status on blood metabolic profile, enzymes and some hormones concentration in the blood of Egyptian buffalo. Investigations were carried out on 24 buffalo (12 pregnant buffalo cows on the 60th day prior to parturition and 12 lactating buffalo cow from 10th day of lactation during winter feeding season). The buffalo cow was average 3 years old and healthy. Buffalo were fed according to Mehlet Moussa Experimental Station which belongs to Animal Production Research Institute, north part of the Nile Delta, Egypt. The present study indicated that there was drop in calcium, sodium, phosphorus and potassium levels during early stage of lactation than pregnant period buffaloes, the opposite trend recorded in chloride levels. 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