Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2014.587.592Bos frontalis)]]>DasK.C. SharmaR. PaulS.S. DhamaK. HaqueN. BaruahK.K. RajkhowaC. 9201499Six fibre degrading fungus were isolated from rumen of mithun (Bos frontalis) at National Research Centre on Mithun, Jharnapani, Medziphema, Nagaland using specific media for rumen fungus. These isolated fungal cultures showed higher fibre degrading enzymes like CM cellulase, Xylanase and Avicelase activity. Addition of fungal culture in the mixed rumen liquor of mithun increased in vitro gas production. The identification and characterization of these isolates was done by DNA isolation, Polymerase Chain Reaction (PCR) amplification, sequencing and plotting of phylogenic tree. The result showed that two isolates were homologus to Orpinomyces species and four isolates were homologus to Neocallimastix species. These isolates can have potential to be used as microbial feed additives in ruminants.]]>Das, K.C., S.S. Paul, L. Sahoo, K.K. Baruah, P.K. Subudhi, K. Ltu and C. Rajkhowa,2014Bos frontalis) fed on mixed tree leaves and rice straw based diet.]]>814261433Lee, S.S., J.K. Ha and K.J. Cheng,2000in vivo ruminal fermentation and nutrient digestion.]]>88201217Menke, K.H. and H. Steingass,1988in vitro gas production using rumen fluid.]]>28755Miller, G.L.,195931426428Paul, S.S., D.N. Kamra, V.R.B. Sastry, N.P. Sahu and N. Agarwal,2004In vitro feed digestion by mixed rumen microflora of buffalo.]]>44313319Sehgal, J.P., D. Jit, A.K. Puniya and K. Singh,200817510518Thareja, A., A.K. Puniya, G. Goel, R. Nagpal, P.K. Singh, J.P. Sehgal and K. Singh,2006In vitro degradation of wheat straw by anaerobic fungi from small ruminants.]]>60412417