Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2014.428.439In vitro Maturation, Fertilization and Embryo Development of Immature
Buffalo Oocytes Vitrified by Different Cryodevice Types]]>KhalilW.A. GabrSh. A. ShamiahSh. M. El-HaifA.M.A. Abdel-KhalekA.E. 7201497This study aimed to compare the efficiency of three cryodevices (Conventional
Straws (CS), Hemi-Straws (HS) and spatula (SP)) in vitrification of buffalo
oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes. They
were vitrified and thawed according to each cryodevice used. Then they were
in vitro matured, fertilized and cultured to blastocyst stage. Survival
and quality, in vitro maturation, fertilization rate and blastocyst production
rates of vitrified immature buffalo oocytes were assessed. Results showed that
total survival rate of the vitrified oocytes was higher (p<0.05) for SP and
HS (96 and 95%) than CS (80%). Recovery rate of normal oocytes relative to vitrified
or survival oocytes was the highest (p<0.05) for SP compared to other cryodevices.
In vitro maturation rate (oocytes at metaphase II stage) was lower (p<0.05)
for all vitrified oocytes than fresh (61.1 vs. 27.8-44%). SP showed higher (p<0.05)
maturation rate (44%) than HS and CS (34.4 and 27.8%, respectively). Cleavage
rate was lower (p<0.05) for all vitrified oocytes than fresh oocytes (37.7-58.9%
vs. 70.8), being higher (p<0.05) for SP and HS (55.8 and 58.9%, respectively)
than for CS (37.7%). Blastocyst production rate relative to cleaved oocytes
was lower (p<0.05) for vitrified than fresh oocytes (5.9-14.3% vs. 31.3)
but the differences among vitrification cryodevice were not significant. In
conclusion, immature buffalo oocytes can vitrified successfully by spatula,
conventional straws and Hemi-straws. Spatula device is a reliable alternative,
inexpensive and easy to assemble for vitrification of immature buffalo oocytes.
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