Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2014.281.291Cirrhinus mrigala]]>NandaP.K. SwainP. NayakS.K. BeheraT. DhamaK. 5201495Enzymatic method using trypsin to establish primary culture from various cells
from heart, liver, kidney, brain, testis, ovary, fin and embryo of Indian Major
Carp, Cirrhinus mrigala was investigated and compared with that of explant
culture method. The dissociation time to obtain cells from individual tissues
and the viability of cells for primary culture varied at 0.125 and 0.25% trypsin
concentration. Single cell culture of trypsinised cells from testis, ovary and
embryo showed good attachment irrespective of trypsin concentration. Cells from
liver and heart were found to be sensitive to trypsin only at higher concentration
(0.25%); whereas, no attachment/or proliferation was observed from cells of
kidney, brain and fin due to sensitivity to trypsin at both the concentrations.
In contrast to this method to obtain primary culture, explant culture from most
of the tissues showed better attachment, resulting in subsequent growth and
proliferation of cells forming monolayer. Overall, the explant culture of most
of the tissues of C. mrigala was found to be suitable and survived more
passages as compared to single cell culture obtained through trypsinisation.
For obtaining primary culture from single cells, further investigations are
desired to identify tissue specific enzymes, standardization of dose, duration
and temperature of enzymatic treatment.]]>Babich, H., M.R. Palace and A. Stern,1993In vitro studies.]]>24173178Bols, N.C. and L.E.J. Lee,19916163187Bols, N.C. and L.E.J. Lee,19941994pp: 145-149pp: 145-149Brown, M.A., C.S. Wallace, C.C. Anamelechi, E. Clermont, W.M. Reichert and G.A. Truskey,20072839283935Chen, S.L., G.C. Ren, Z.X. Sha and C.Y. Shi,2004Paralichthys olivaceus for virus isolation.]]>60241246Collett, J., A. Crawford, P.V. Hatton, M. Geoghegan and S. Rimmer,20074117126Cruz, H.J., E.M. Dias, J.L. Moreira and M.J.T. Carrondo,199747482488Freshney, R.I.,20055th Edn.,pp: 387-389pp: 387-389Ganassin, R.C., K. Schirmer and N.C. Bols,20002000pp: 631-651pp: 631-651Glade, C.P., B.A. Seegers, E.F. Meulen, C.A. van Hooijdonk, P.E. van Erp and P.C. van de Kerkhof,1996288203210Hightower, L.E. and J.L. Renfro,1988248290302Huang, H.L., H.W. Hsing, T.C. Lai, Y.W. Chen and T.R. Lee et al.,20102010Joseph, M.A., R.K. Sushmitha, C.V. Mohan and K.M. Shankar,19987514031406Lai, Y.S., J.A.C. John, C.H. Lin, I.C. Guo, S.C. Chen, K. Fang and C.Y. Chang,2003Epinephelus awoara (Temminck and Schlegel) and their susceptibility to grouper irido‐and nodaviruses.]]>263142Lakra, W.S., M.R. Behera, N. Sivakumar, M. Goswami and R.R. Bhonde,2005Labeo rohita (Hamilton).]]>52373376Lakra, W.S. and R.R. Bhonde,1996Labeo rohita (Ham.).]]>9149152Ma, C. and P. Collodi,1999213946Mastan, S., S. Goswami, V. Kakaria and T. Qureshi,2007Cyprinus carpio (Linn).]]>710531056McKeehan, W.L.,19771335343Melican, D., R. Butler, N. Hawkins, L.H. Chen and E. Hayden et al.,20056315491563Mitalipova, M.M., R.R. Rao, D.M. Hoyer, J.A. Johnson and L.F. Meisner et al.,2005231920Nanda, P.K., P. Swain, S.K. Nayak, S.S. Mishra, P. Jayasankar and S.K. Sahoo,2014In vitro growth of fibroblast-like cells of Indian Major carp, Cirrhinus mrigala.]]>2177182Nanda, P.K., P. Swain, S.K. Nayak, S. Dash, P. Routray, S.K. Swain and B.C. Patra,2009Cirrhinus mrigala.]]>45148151Nicholson, B.L.,198039394397Noga, E.J.,198065556Ossum, C.G., E.K. Hoffmann, M.M. Vijayan, S.E. Holt and N.C. Bols,20046411031116Part, P. and F. Bergstrom,19951995pp: 207-227pp: 207-227Pleskach, V.A., I.V. Kozhukharova, I.V. Artsybasheva, T.A. Goilo, M.V. Tarunina and N.A. Filatova,199336806815 (In Russian)Pumper, R.W., P. Fagan and W.G. Taylor,19716266268Rao, K.S., M.A. Joseph, K.M. Shanker and C.V. Mohan,199773374376Rathore, G., G. Kumar, T.R. Swaminathan, N. Sood, V. Singh, R. Abidi and W.S. Lakra,2007Labeo rohita (Ham.).]]>5419397Sathe, P.S., D.T. Mourya, A. Basu, S.S. Gogate and K. Banerjee,1995Cirrhinus mrigala.]]>33589594Singh, I.S.B., P. Rosamma, M. Raveendranath and J. Shanmugam,1995Heteropneustes fossilis.]]>33595599Smets, L.A., C. Homburg and H. van Rooy,19793107111Sutradhar, B.C., J. Park, G. Hong, S.H. Choi and G. Kim,201030232238Swain, P., P.K. Nanda, S.K. Nayak and S.S. Mishra,20142110Villena, A.J.,200313111140Wolf, K. and M.C. Quimby,19691969pp: 253-306pp: 253-306Wolf, K. and M.C. Quimby,19762453456Wolf, K. and M.C. Quimby,19762471474Yanase, Y., H. Suzuki, T. Tsutsui, I. Uechi, T. Hiragun, S. Mihara and M. Hide,200723562567Ye, H.Q., S.L. Chen, Z.X. Sha and M.Y. Xu,2006Lateolabrax japonicus.]]>69115126