Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2014.262.269Brucella abortus
from Cattle by RBPT, STAT and PCR and Sample Suitability of Whole Blood
for PCR]]>KarthikK. RathoreR. ThomasP. ElamuruganA. ArunT.R. DhamaK. 4201494Brucella abortus, one of the major pathogen causing abortions in cattle
worldwide and a zoonotic agent, need to be detected earlier in order to prevent
its spread among animals. The present study aimed at to know the prevalence
of B. abortus in cattle population of three states (Uttar Pradesh, Uttarakhand
and Tamil Nadu) of India by serological (Rose Bengal Plate Test (RBPT) and Serum
Tube Agglutination Test (STAT)) and molecular (polymerase chain reaction) detection
in sera samples and whole blood (n = 370), respectively. Out of a total of 370
sera samples, 61 (16.49%) were positive by RBPT and 59 (15.94%) by STAT. Screening
of the whole blood samples by genus specific bcsp31 gene based PCR as
well as species specific IS711 gene based PCR revealed that 56 (15.13%)
samples were positive for brucellosis. None of the serologically negative sample
showed positivity by PCR; however few positive samples were tested negative
by PCR. Sensitivity and specificity of PCR compared with RBPT was 100 and 92.4%
while with STAT these were 100 and 95.16%, respectively. Results are promising
that whole blood can be used for studying the molecular epidemiology of B.
abortus in cattle and particularly detecting the active phase of infection
and PCR can be well adopted as a valuable test for mass screening of animals
for this purpose. The present study adds to the prevalence data available regarding
to B. abortus infection in cattle population and highlights the usefulness
and advantages of molecular tool of PCR over serological tests.]]>Al Nakkas, A.F., S.G. Wright, A.S. Mustafa and S. Wilson,200296397403Alton, G.G., L.M. Jones and D.E. Pietz,19752nd Edn., WHO Monograph Series, No. 454.Atluri, V.L., M.N. Xavier, M.F. de Jong, A.B. den Hartigh and R.E. Tsolis,2011Brucella species with their hosts.]]>65523541Baily, G.G., J.B. Krahn, B.S. Drasar and N.G. Stoker,1992Brucella melitensis and Brucella abortus by DNA amplification.]]>95271275Bhanu Rekha, V., L. Gunaseelan, A. Subramanian and G. Yale,20136681685Da Silva Mol, J.P., S.A. Franca, T.A. da Paixao and R.L. Santos,201219117126Dhama, K., K. Karthik, S. Chakraborty, R. Tiwari, S. Kapoor, A. Kumar and P. Thomas,201417151166Doust, S.R.H., Z. Ahamdi, A. Ahamdi, M. Hajia, M. Izadi and A.M. Mobarez,2007Brucella abortus by alkB and IS711 based primers.]]>126267Habtamu, T.T., R. Rathore, K. Dhama and K. Karthik,2013Brucella melitensis from disease outbreak in sheep and Brucella abortus from cattle farm by IS711 and Omp2a gene based PCR.]]>519201925Gemechu, M.Y., J.P. Gill, A.K. Arora, S. Ghatak and D.K. Singh,20112170177Guarino, A., L. Serpe, G. Fusco, A. Scaramuzzo and P. Gallo,2000Brucella species in buffalo whole blood by gene-specific PCR.]]>147634636Gupta, V.K., S. Nayakwadi, A. Kumar, K. Gururaj, A. Kumar and R.S. Pawaiya,201423139Keid, L.B., R.M. Soares, S.A. Vasconcellos, V.R. Salgado, J. Megid and L.J. Richtzenhain,20101679699Khamesipour, F., A. Doosti and H. Taheri,20137495500Kumar, N., B.C. Pal, S.K. Yadav, A.K. Verma, U. Jain and G. Yadav,20097129131Leal-Klevezas, D.S., I.O. Martinez-Vazques, J. Garcia-Cantu, A. Lopez-Merino and J.P. Martinez-Soriano,2000Brucella abortus biovar 1 in infected goats.]]>759197Mitka, S., C. Anetakis, E. Souliou, E. Diza and A. Kansouzidou,20074512111218Morgan, W.J. and D.G. MacKinnon,19791979pp: 171-198pp: 171-198Moussa, I.M., M.E. Omnia, A.S. Amin, M.H. Ashgan and S.A. Selim,2011Brucella species.]]>515111520O'Leary, S., M. Sheahan and T. Sweeney,2006Brucella abortus detection by PCR assay in blood, milk and lymph tissue of serologically positive cows.]]>81170176Priyadarshini, A., L.N. Sarangi, T.K. Palai, H.K. Panda, R. Mishra and P.C. Behera,2013732553260Queipo-Ortuno, M.I., F. Tena, J.D. Colmenero and P. Morata,2008Brucella DNA from spiked human serum samples using real-time PCR.]]>27109114Rodriguez, J.C., E. Fuentes and G. Royo,1997105612616Sam, I.C., R. Karunakaran, A. Kamarulzaman, S. Ponnampalavanar and S.F. Syed Omar et al.,2012Brucella melitensis in a diagnostic laboratory.]]>80321325Sambrook, J. and D.W. Russell,20013rd Edn.,Pages: 2344Pages: 2344Seleem, M.N., S.M. Boyle and N. Sriranganathan,2010140392398Soleimani, M., S. Shams and K. Majidzadeh-A,2013115828834Taleski, V.,20103239245Islam, M.R.U., M.P. Gupta, G. Filia, P.K. Sidhu and T.A. Shafi et al.,2013158Trangadia, B., S.K. Rana, F. Mukherjee and V.A. Srinivasan,201042203207Wattam, A.R., K.P. Williams, E.E. Snyder, N.F. Almeida and M. Shukla et al.,200919135693579Yu, W.L. and K. Nielsen,2010Brucella spp. by polymerase chain reaction.]]>51306313