Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2014.243.252Arcobacter butzleri and Arcobacter cryaerophilus
in Clinical Samples of Humans and Foods of Animal Origin by Cultural and Multiplex
PCR Based Methods]]>RameesThadiyam Puram RathoreRamswaroop Singh BagalkotPrashanth Suresh MohanHosakote Venkatappa KumarAshok DhamaKuldeep 4201494In recent years, the frequency of isolation and detection of Arcobacter
organisms from animals and humans with enteritis and food samples, highlights
the importance of arcobacters worldwide as emerging food-borne pathogens. Reports
are very scanty regarding prevalence of arcobacters from India. Therefore, the
present study aimed to know the prevalence of Arcobacter spp. (Arcobacter
butzleri and Arcobacter cryaerophilus) in humans and foods of animal
origin by employing cultural and multiplex PCR (mPCR) methods. A total number
of 353 samples were collected from human hospitals, retail meat shops and milk
suppliers [human stools (102), chicken meat (151), milk (100)] from in and around
Bareilly region, Uttar Pradesh, India. By cultural method the overall prevalence
rate of Arcobacter spp. was found to be 10.20% (36/353) while it was
18.13% (64/353) with mPCR which revealed mPCR to be a more efficient technique
in detecting arcobacters. The highest prevalence rate was observed in chicken
meat, followed by human stool and milk samples with A. butzleri having
more prevalence. For simultaneous detection and differentiation of arcobacters
at species level the cultural methods possess limitations while mPCR gave rapid
and confirmatory detection of A. butzleri and A. cryaerophilus
species. The results of the study add to the epidemiological data available
for arcobacters. Extensive epidemiological studies employing the utility of
mPCR are suggested for knowing the magnitude of Arcobacter infection
animals, humans and various food sources in the country. This would help in
designing appropriate prevention and control strategies for this important pathogen
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