Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2013.461.472ChuChishih LinHengching SuYaochi 3201383Tuberculosis (TB) is a zoonotic disease affecting mammals worldwide. Herbivore
TB is caused by Mycobacterium bovis and other Nontuberculous Mycobacteria
(NTM). This study aimed to investigate the prevalence of mycobacterial species
and to evaluate the diagnostic methods in detecting mycobacterial infection
in Intradermal Tuberculin Test (ITT) positive animal. Samples were collected
from ITT positive animals (151 cattle and 36 deer) for mycobacterial isolation,
pathological examination, acid-fast stain, molecular diagnosis and ELISA. The
detection rate of mycobacterial infection in tissues differed among methods
from highest in gross lesion (105, 69.5%), Mn-PCR (89, 64.2%), histopathology
(89, 58.9%) to acid fast acid (84, 55.6%) in cattle and from Mn-PCR (28, 77.8%),
gross lesion and histopathology (19, 52.8%), to acid fast stain (47.2%) in deer.
Among 64 culture positive, 48 samples were infected by M. bovis (30 in
cow vs. 18 in deer). ELISA examination found high level of M. paratuberculosis
infection (67, 44.4%) in cattle and M. bovis (18, 52.8%) in deer. Among
gross lesion, histopathology, acid fast stain and Mn-PCR methods, the most prevalent
tissues of mycobacterial infection were retropharynx lymph node and mediastinal
lymph nodes in cattle and retropharynx lymph node and lung in deer. Farm with
only once ITT in routine test may be not true M. bovis infected and ITT
was overestimated in national MTB clearance program and the reliability in Taiwan.
DNA sequence analysis of M. bovis demonstrated identical genotypes between
deer and cattle from Yulin and diverse origins among cattle from three counties.]]>Amadori, M., K.P. Lyashchenko, M.L. Gennaro, J.M. Pollock and I. Zerbini,200285379389Aranaz, A., L. De Juan, J. Bezos, J. Alvarez and B. Romero et al.,2006Mycobacterium bovis and M. avium subsp. paratuberculosis.]]>37593606Araujo, C.P.D., C.Q.F. Leite, K.A.D. Prince, K.D.S.G. Jorge and A.L.A.R. Osorio,2005Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil.]]>100749752Ayele, W.Y., S.D. Neill, J. Zinsstag, M.G. Weiss and I. Pavlik,20048924937Chia, M.Y.,2001Mycobacterium tuberculosis, M. bovis, M. avium subsp. avium and M. avium subsp. paratuberculosis infection by multiplex-PCR.]]>2001Chiu, P.C.,20042004Cho, S.N.,1998Mycobacterium bovis and use in the serodiagnosis of bovine tuberculosis.]]>22103112Chu, C.S., C.Y. Yu, C.T. Chen and Y.C. Su,2012Mycobacterium tuberculosis and M. bovis infection in feedlot deer (Cervus unicolor swinhoei and C. nippon taiouanus) in Taiwan.]]>45426434De La Rua-Domenech, A.T. Goodchild, H.M. Vordermeier, R.G. Hewinson, K.H. Christiansen and R.S. Clifton-Hadley,200681190210Fifis, T., L.A. Corner, J.S. Rothel and P.R. Wood,1994Mycobacterium bovis antigens.]]>39267274Iwamoto, T., T. Sonobe and K. Hayashi,2003Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples.]]>4126162622Jeon, B.Y., S.C. Kim, S. Je, J. Kwak and J.E. Cho et al.,201088390393Jiang, T.S.,19931993Jones, T.C., R.D. Hunt and N.W. King,19971997pp: 413-503pp: 413-503Jou, R., W.L. Huang and C.Y. Chiang,2008Mycobacterium bovis Taiwan.]]>14515517Kim, B.J., S.K. Hong, K.H. Lee, Y.J. Yun and E.C. Kim et al.,2004Mycobacterium tuberculosis complex and non tuberculous mycobacteria by Duplex PCR assay using the RNA polymerase gene (rpoB).]]>4213081312Kunze, Z.M., S. Wall, R. Appelberg, M.T. Silva, F. Portaels and J.J. McFadden,1991Mycobacterium avium.]]>522652272Lauzi, S., D. Pasotto, M. Amadori, I.L. Archetti, G. Poli and L. Bonizzi,20001601724Liebana, E., R.M.Girvin, M. Welsh, S.D. Neill and J.M. Pollock,1999Mycobacterium bovis and mycobacterial antigen in experimental bovine tuberculosis.]]>6710341044Ngandolo, B.N., B. Muller, C. Diguimbaye-Djaibe, I. Schiller and B. Marg-Haufe et al.,2009898189Pfyffer, G.E., B.A. Bwrown-Elliott and R.J. Wallace,2007Mycobacterium, General Characteristics, Isolation and Staining Procedures.]]>2007pp: 532-559pp: 532-559Rhyan, J.C., D.A. Saari, E.S. Williams, M.W. Miller, A.J. Davis and A.J. Wilson,1992Cervus elaphus nelsoni) in Colorado.]]>4428433Romero, R.E., D.L. Garzon, G.A. Mejia, W. Monroy, M.E. Patarroyo and L.A. Murillo,1999Mycobacterium bovis in bovine clinical samples by PCR species-specific primers.]]>63101106Rothel, J.S., S.L. Jones, L.A. Corner, J.C. Cox and P.R. Wood,199067134137Soo, P.C., Y.T. Horng, K.C. Chang, J.Y. Wang and P.R. Hsueh et al.,2009Mycobacterium tuberculosis and Mycobacterium tuberculosis complex from clinical specimens.]]>23240246Vansnick, E., P. de Rijk, F. Vercammen, D. Geysen, L. Rigouts and F. Portaels,2004Mycobacterium avium subspecies paratuberculosis.]]>100197204Varges, R., C.D. Marassi, W. Oelemann and W. Lilenbaum,200986371372Wards, B.J., D.M. Collins and G.W. de Lisle,1995Mycobacterium bovis in tissues by polymerase chain reaction.]]>43227240Whelan, C., E. Shuralev, G. O'Keeffe, P. Hyland and H.F. Kwok et al.,2008Mycobacterium bovis infection in cattle.]]>1518341838Wood, P.R., L.A. Corner, J.S. Rothel, C. Baldock and S.L. Jones et al.,199168286290OIE,20092009pp: 1-16pp: 1-16Wu, Y.H.,19891st Edn.,pp: 141-145pp: 141-145