Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2013.333.340Tachypleus gigas (Muller, 1785) Eggs in Sand and Water Media]]>FaizulM.I.M. FaizalM.M. ChristianusA. AminS.M.N. 2201382In order to develop suitable methods to successfully hatch Tachypleus gigas eggs, a study was carried out on different salinity and culture media. The main objective for this study was to determine the effect of watering frequency, salinity and media on the incubation period and hatching of T. gigas eggs. This research consisted of three experimental studies. In the first experiment, effect of water salinities (15, 20, 25 and 30 ppt) for watering or moistening sand was studied. For the second study, effect of watering frequencies (once in 1, 3 and 6 days) on the eggs incubated in sand were investigated. As for the last experiment, effect of incubation medium (water and sand) on eggs were compared. Data collected for these experiments were eggs diameter and hatching percentages. Embryonic developments were observed and photographed during the study period. Results from experiment 1, showed that at the end of the incubation period, watering with water salinity of 25-30 ppt produced significantly larger eggs diameter (p<0.05) while percentages of hatching was the highest with 30 ppt water. In experiment 2, it was found that percentages of hatching were significantly higher (p<0.05) when watered once a day and in three days. As for experiment 3, at the end of the incubation period, there was no significant different (p>0.05) in the eggs diameter and percentage of hatching between sand and water medium. In conclusion, the most suitable salinity and watering frequency were 25-30 ppt and once in 3 days, respectively. However, both sand and water are suitable media to successfully incubate T. gigas eggs. Overall, this study showed that T. gigas eggs can hatch as early as 40 days after fertilization.]]>Botton, M.L.,1984Limulus polyphemus in Delaware Bay, New Jersey, USA.]]>81199207Botton, M.L. and T. Itow,20092009pp: 439-454pp: 439-454Chatterji, A., S. Kotnala and R. Mathew,2004Tachypleus gigas (Muller).]]>87248250Chezhian, A., D. Senthamilselvan and N. Kabilan,2012Cyprinus carpio var. communis (Linnaeus).]]>7588596Christianus, A. and C.R. Saad,2010Tachypleus gigas in Malaysia.]]>2010Cohen, J.A. and H.J. Brockmann,1983Limulus polyphemus.]]>33274281Iwanaga, S. and S. Kawabata,19983973984Karl, B.,20042004Faizul, M.M.I., A. Christianus, S.M.N. Amin, S. Shafeeqa and T. Rishzuan,2011Tachypelus gigas (Muller, 1785) under two different culture system.]]>6788794Morton, B. and G. Blackmore,20014212361263Penn, D. and H.J. Brockmann,1994Limulus polyphemus.]]>187373384Rudloe, A.,1979Limulus polyphemus: A Review of the Ecologically Significant Literature.]]>1979pp: 27-35pp: 27-35Saigusa, M.,1996191234240Sasi, H.,2011Pseudophoxinus anatolicus (hanko, 1924) (cyprinidae) from anatolia.]]>6238241Shuster, C.N.,1982Limulus polyphemus, with Reference to other Limulidae.]]>1982pp: 1-52pp: 1-52Sekiguchi, K.,1988Pages: 428Pages: 428Sekiguchi, K., H. Seshimo and H. Sugita,19881119Zaleha, K., I. Hazwani, H.S. Hamidah, B.Y. Kamaruzzaman and K.C.A. Jalal,2011Tachypleus gigas (Muller, 1785) in laboratory culture.]]>1126202626Ehlinger, G.S. and R.A. Tankersley,2003292199212