Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2012.1041.1048Staphylococcus aureus]]>CaoDuoyao JingXiaoqi WangXiaoyu LiuHui ChenDekun 102012710Staphylococcus aureus is one of the major pathogen which can cause mastitis in dairy animal. CD4+ lymphocytes play a significant role in clearing this type of bacteria. To our knowledge, CD4+ lymphocytes change in mouse model of S. aureus mastitis is poorly understood. To investigate the response of CD4+ lymphocytes in mammary gland, the mice mastitis was induced with S. aureus in this study. New protocol was used to separate lymphocytes in mammary gland. The results showed that CD4+ lymphocytes increased significantly comparing with blank and saline mice (p = 0.0019, p<0.01) at 72 h post Intramammary Inoculation (IMI). At the same time, the infection mice mammary gland tissues were swelled and were suffering from hyperemia. From histological observation, massive inflammatory cells are infiltrated in the tissue. The results suggest that CD4+ lymphocyte is one of important cells in against S. aureus infection in mammary gland.]]>Barkema, H.W., Y.H. Schukken and R.N. Zadoks,2006Staphylococcus aureus mastitis.]]>8918771895Bharathan, M. and I.K. Mullarky,201116409419Bougarn, S., P. Cunha, F.B. Gilbert, A. Harmache, G. Foucras and P. Rainard,201156749759Bushnell, R.B.,19846361370Chandler, R.L.,19703273282Corradin, C. and A. Lanzavecchia,19917139147Haltia, L., T. Honkanen-Buzalski, I. Spiridonova, A. Olkonen and V. Myllys,2006482222Islander, U., A. Andersson, E. Lindberg, I. Adlerberth, A.E. Wold and A. Rudin,201078381386Jing, X., Y. Han, D. Cao, S. Mou and H. Liu et al.,201211597602Joshi, A., G. Pancari, L. Cope, E. Bowman, D. Cua, R. Proctor and T. McNeely,20128326336Kehrli, Jr. M.E. and J.A. Harp,200117495516Lawrence, P.K., B. Rokbi, N. Arnaud-Barbe, E.L. Sutten, J. Norimine, K.K. Lahmers and W.C. Brown,2012Staphylococcus aureus Newman strain identified following immunization with heat-killed bacteria.]]>19477489Lin, L., A.S. Ibrahim, X. Xu, J.M. Farber and V Avanesian et al.,20092009McLoughlin, R.M., R.M. Solinga, J. Rich, K.J. Zaleski and J.L. Cocchiaro et al.,2006+ T cells and CXC chemokines modulate the pathogenesis of Staphylococcus aureus wound infections.]]>1031040810413Myllys, V., T. Honkanen-Buzalski, P. Huovinen, M. Sandholm and E. Nurmi,199435363369Osteras, O., V.L. Edge and S.W. Martin,19998212211231Prabhakara, R., J.M. Harro, J.G. Leid, A.D. Keegan, M.L. Prior, M.E. Shirtliff and A.J. Baumler20117950105018Proctor, R.,2011Staphylococcus aureus vaccine?]]>1929212927Puel, A., S. Cypowyj, J. Bustamante, J.F. Wright and L. Liu et al.,20113326568Riollet, C., P. Rainard and B. Poutrel,2000480247258Rivas, A.L., F.W. Quimby, O. Coksaygan, L. Olmstead and D.H. Lein,200064232237Salman, S., A. Khol-Parisini, H. Schafft, M. Lahrssen-Wiederholt, H.W. Hulan, D. Dinse and J. Zentek,2009102134Seyffert, N., C. Le Marechal, J. Jardin, J.A. McCulloch and F.R. Rosado et al.,2012Staphylococcus aureus proteins differentially recognized by the ovine immune response in mastitis or nasal carriage.]]>2012Shafer-Weaver, K.A., C.M. Corl and L.M. Sordillo,19998216961706Shitandi, A. and G. Kihumbu,2004559Shitandi, A. and A. Sternesjo,20048741454149Sinha, P., A.K. Ghosh, T. Das, G. Sa and P.K. Ray, 1999Staphylococcus aureus evokes Th1 type response in mice.]]>67157165Smith, R.P., A.L. Baltch, W.J. Ritz, P.B. Michelsen and L.H. Bopp,201051274277Trigo, G., M. Dinis, A. Franca, E.B. Andrade, R.M. Gil da Costa, P. Ferreira and D. Tavares,2009Streptococcus agalactiae mastitis.]]>58951958Weigmann, B., I. Tubbe, D. Seidel, A. Nicolaev, C. Becker and M.F. Neurath,2007223072311Leitner, G., O. Krifucks, A. Younis, E.D. Heller and A. Saran,2002in vitro.]]>49354360