Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2011.844.850MoriNobuko LeePeter YamamotoIchiro NozawaSatoshi AraiToshiro 8201168The aim of this study was to determine what insulin-induced daily changes occur with adiponectin, Tumor Necrosis Factor-alpha (TNF-α) and other relevant lipid metabolic parameters such as Free Fatty Acid (FFA), Total Cholesterol (T-Cho) and Triglyceride (TG) levels in dogs suffering from type 1 Diabetes Mellitus (T1DM). Six dogs were treated with insulin injections at the Nippon Veterinary and Life Science University Animal Clinical Center for T1DM. They were clinically diagnosed as T1DM by signs of polydipsia, polyuria, glycosuria and high fasting plasma glucose (over 250 mg dL-1). Blood samples of dogs were collected twice a day, before breakfast and after 6 hours of insulin injection. As results, post-insulin treatment value of TG, BUN, creatinin and chyromicron significantly increased, whereas ALT, ALP, protein and FFA significantly decreased post treatment. Significant reductions in total cholesterol, Very Low- Density Lipoprotein-cholesterol (VLDL) and Low-Density Lipoprotein-cholesterol (LDL) concentrations were also observed. Phospholipid Transfer Protein (PLTP) activity increased post-insulin treatment. The increase in Cholesteryl Ester Transfer Protein (CETP) activity was significant. Remarkably, insulin treatment reduced plasma adiponectin levels in T1DM dogs. Insulin resistance results in an increased risk of atherosclerosis on a long term basis as evidenced by atherogenicity markers of the plasma lipoprotein profile such as increased CETP and PLTP activities. Under obese condition adiponectin has been shown to negatively correlate with several inflammatory mediators (IL-1β, IL-6, IL-8, TNF-α). Low-grade chronic systemic inflammation has been associated with obesity potentially leading to insulin resistance.]]>Assal, H.S., M. Fath-Allah and A. Elsherbiny,20077865869Arai, T., M. Nakamura, E. Magori, H. Fukuda and T. Sako,200374183185Bauer, J.E.,2004224668675Boni-Schnetzler, M., S. Boller, S. Debray, K. Bouzakri and D.T. Meier et al.,200915052185229Chapman, M.J.,19861986pp: 70-143pp: 70-143Abd El-Ghaffar, N. and N.H. El-Said,,20066626630Guyard-Dangremont, V., C. Desrumaux, P. Gambert, C. Lallemant and L. Lagrost,1998120517525Hsiao, P.J., K.K. Kuo, S.J. Shin, Y.H. Yang and W.Y. Lin et al.,20072221182123Hotta, K., T. Funahashi, Y. Arita, M. Takahashi and M. Matsuda et al.,20002015951599Ishioka, K., A. Omachi, M. Sagawa, H. Shibata, T. Honjoh, K. Kimura and M. Saito,200680127132Lagrost, L., A. Athias, N. Lemort, J.L. Richard and C. Desrumaux et al.,1999143415425Mahley, R.W. and K.H. Weisgraber,197435713721Moerland, M., H. Samyn, T. van Gent, R. van Haperen and G. Dallinga-Thie et al.,20082812771282Mori, A., P. Lee, T. Sako, H. Mizutani and T. Arai,20077110171026Radin, M.J., L.C. Sharkey and B.J. Holycross,200938136156Raja, M.M.M., A. Raja, M.M. Imran, A.M.I. Santha and K. Devasena,2011105159Roth, C.L., M. Kratz, M.M. Ralston and T. Reinehr, 20102010Tall, A.,199564235258Terpstra, A.H., F.J. Sanchez-Muniz, C.E. West and C.J. Woodward,198271669673Watson, T.D.G.,199661723Whitehead, J.P., A.A. Richards, I.J. Hickman, G.A. Macdonald and J.B. Prins,20068264280Yu, J.G., S. Javorschi, A.L. Hevener, Y.T. Kruszynska, R.A. Norman, M. Sinha and J.M. Olefsky,20025129682974Zeller, M., D. Masson, M. Farnier, L. Lorgis and V. Deckert et al.,20075019481955