Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2011.362.370LiHongwei ZhaoChunjiang XuHaiyue YuBo WuChangxin 4201164Classic taxonomy of animal mainly depends on genetic, morphology, fossil and distribution studies, so the study aims at developing a method which can rapidly identify to what species unknown animals are closely related by comparison of genomic sequences. With more complete sequenced animal genomes available, genomic information will play a key role on classifying unknown animals. Here, we put forward a method how to rapidly identify unknown animals. The method includes: (1) selecting a vector which is convenient for cloning and sequencing; (2) digesting the total genomic DNA from unknown animals and the vector with the same two enzymes which have recognition sites on the vector; (3) ligating the digested DNA to the vector; (4) transforming the ligation products to competent cell; (5) selecting positive clones to sequence and blast with sequenced animal genomes; (6) then finding out the most close relative species to the unknown animals. In the paper we took a kind of unknown wild flies which captured in nature, for example, to display the method. By analysis of data from blast output, results showed that eleven query sequences (1, 2, 4, 5, 6, 7, 9, 10, 12, 13, 14) from unknown flies have the highest similarities with subject sequences on the D. ananassae genome, but only minor parts of No. 2, 4~7, 9, 10 and 12 sequences have similarities with subject sequences on eleven drosophila genomes and the unknown flies are the most closely related to D. ananassae in evolutionary contexts. it will be a promising power in rapidly identifying to what species unknown animals is closely related when more complete sequenced animal genomes are available .]]>Adams, M.D., S.E. Celniker, R.A. Holt, C.A. Evans and J.D. Gocayne et al.,2000Drosophila melanogaster.]]>28721852195Brown, B., R.M. Emberson and A.M. Paterson,1999Weiseana(Lepidoptera, Hepialidae) species identification.]]>89287294Bucklin, A., M. Guarnieri, R.S. Hill, A.M. Bentley and S. Kaartvedt,1999401239254Mcbride, C.S. and J.R. Arguello,2007Drosophila genomes reveal nonneutral evolution and the signature of host specialization in the chemoreceptor superfamily.]]>17713951416Eisen, J.A.,19988163167Eisen, J.A. and P.C. Hanawalt,1999435171213Eisen, J.A.,200010606611Folmer, O., M. Black, W. Hoeh, R. Lutz and R. Vrijenhoek,1994c oxidase subunit I from diverse metazoan invertebrates.]]>3294299Bosco, G., P. Campbell, J.T. Leiva-Neto and T.A. Markow,2007Drosophila species genome size and satellite DNA content reveals significant differences among strains as well as between species.]]>17712771290Hebert, P.D.N., A. Cywinska, S.L. Ball and J.R. deWaard,2003270313321Hebert, P.D.N., S. Ratnasingham and J.R. de Waard,2003270S596S599Henikoff, S. and J.G. Henikoff,1992891091510919Hotopp, J.C., M.E. Clark, D.C.S.G. Oliveira, J.M. Foster and P. Fischer et al.,200731717531756Lawrence, J.G. and H. Ochman,1998Escherichia coli genome.]]>9594139417Koski, L.B. and G.B. Golding,200152540542Noor, M.A.F., D.A. Garfield, S.W. Schaeffer and C.A. Machado,2007Drosophila pseudoobscura and D. persimilis genome sequences in relation to chromosomal inversions.]]>17714171428Mount, D.W.,20042nd Edn.,Nelson, K.E., R.A. Clayton, S.R. Gill, M.L. Gwinn and R.J. Dodson et al.,1999Thermotoga maritima.]]>399323329O'Grady, P.M. and T.A. Markow,200931014Qian, Y.H., Y.L. Liu, S.T. Li, Y. Yang and Q.T. Zeng,200628397402Reich, J.G., H. Drabsch and A. Diumler,19841255295543Stark, A., M.F. Lin, P. Kheradpour, J.S. Pedersen and L. Parts et al.,2007Drosophila genomes using evolutionary signatures.]]>450219232Karlint, S. and S.F. Altschul,19908722642268Sicheritz-Ponten, T. and G.E. Anderson,200129545552Sullivan, W., M. Ashburner and S. Hawley,2000Drosophila Protocols.]]>Rohde, C., A.C. Garcia, V.H. Valiati and V.L. Valente,20061267788Coyne, J.A.,199347778788Sucena, E. and D.L. Stern, 2000Drosophila sechellia and its sibling species caused by cis-regulatory evolution of ovo/shaven-baby.]]>9745304534Gao, J.J., H.A. Watabe, T. Aotsuka, J.F. Pang and Y.P. Zhang,2007Drosophila obscura species group, with emphasis on the old world species.]]>78787Altschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman,19972533893402