Asian Journal of Animal and Veterinary Advances1683-99191996-3289Academic Journals Inc.10.3923/ajava.2009.306.313Oncorhynchus mykiss) using Plasma Cortisol and Glucose Responses as Physiological Stress Indicators]]>SattariA. MirzargarS.S. AbrishamifarA. LourakzadeganR. BahonarA. MousaviH.E. NiasariA. 6200946This study investigates Alternating Current (AC) electroanesthesia of rainbow trout (Oncorhynchus mykiss) in comparison with MS222 and clove oil, using plasma cortisol and glucose concentrations as stress assessment indicators. A microcontroller-based apparatus was designed and constructed to allow a programmable voltage-time Pulse-Width Modulated (PWM) electrical wave application through 19x20 cm submersible electrodes for 91sec in a 33 cm long tank to induce loss of equilibrium and immobility with recovery after 52±27 sec. Recovery after 660±102 sec was observed in MS222-anesthetized fish (after induction for 720±72 sec) and a recovery time of 546±102 sec was observed in clove oil-anesthetized fish (after induction for 144±42 sec) both are significantly longer recovery times in comparison with electroanesthesia (p<0.001). Using direct enzyme-linked immunosorbant assay (ELISA) for cortisol and enzymatic colorimetric assay for glucose assessments at 0, 1, 6, 12 h after each anesthesia, the anesthetics indicated similar trend of cortisol responses during 12 h of investigation. The dilatory trend of glucose changes and response derived from anesthetics and electricity and its surge at 6 h after anesthesia (p<0.05) confirmed glucose as a second order indicator of stress responses. Electroanesthesia is a fast, economic, eco-friendly and safe anesthetic method provides desirable trout immobility for aquaculture activities.]]>Adetunji, O.R., J.O. Adeleye, B.L. Salako and M.A. Kuti,2008256668Andersen, J.B. and T. Wang,2002Bufo marinus.]]>131639646Arnolds, D.E.W., S.J. Zottoli, C.E. Adams, S.M. Dineen, S. Fevrier, Y. Guo and A.J. Pascal,2002203188189Barton, B.A. and W.P. Dwayer,1997519981008Barton, B.A. and G.K. Iwama,19911326Barton, B.A. and R.E. Barton,1982Salmo Gairdneri Richardson to various transport condition anesthesia and cold shock.]]>203951Barton, B.A.,200242517525Chiba, H., T. Hattori, H. Yamada and M. Iwata,200672693695Hill, J.V., W. Davidson and M.E. Forster,2002Oncorhynchus tshawytsha).]]>271928Jenning, C.A. and G.L. Looney,199818187190Kiessling, A., D. Johansson, I.H. Zahl and O.B. Samuelsen,2009Salmo salar) following bath administration.]]>286301308Ortuńo, J., M.A. Esteban and J. Meseguer,2002Sparus auratus L.).]]>124959Palić, D., D.M. Herolt, C.B. Andreasen, B.W. Menzel and J.A. Roth,2006Pimephales promelas Rafinesque 1820).]]>254675685Park, M.O., W.J. Hur, S.Y. IM, D.W. Seol, J. Lee and I.S. Park,2008Epinephelus bruneus.]]>39877884Patino, R., J.M. Redding and C.B. Schreck,1987Oncorhynchus kisutch).]]>68431439Pickering, A.D.,1981Pages: 367Pages: 367Pickering, A.D. and T.G. Pottinger,19897253258Ross, L.G., B. Ross and B. Ross,20083rd Edn.,Pages: 240Pages: 240Robertson, L., P. Thomas and C.R. Arnold,1998Sciaenops ocellatus) to several transportation procedures.]]>68115130Singley, J.A. and W. Chavin,1975Carassius auratus L.).]]>507782Sink, T.D., R.T. Lochmann and K.A. Fecteau,20083495101Small, B.C.,2003Ictalurus punctatus.]]>218177185Small, B.C. and K.B. Davis,2007Ictalurus punctatus.]]>33184187Soltani, M., R. Omidbaigi, S. Rezvani, M.R. Mehrabi and H. Chitsaz,2002568589Son, M.H., M.W. Park, J.I. Myeong, D.J. Kim, B.H. Kim, Q.T. Jo and I.G. Jeon,2001Sebastes schlegeli produced for wild stock enhancement.]]>23285290Summerfelt, R.C. and S.S. Lynwood, 19901990pp: 213-272pp: 213-272Wagner, E., R. Arndt and B. Hilton,2002211353366Cho, G.K. and D.D. Heath,2000Onchorhynchus tshawytscha (Walbaum).]]>31537541Tort, L., M. Puigcerver, S. Crespo and F. Padros,200233907910