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coli and Bifidobacterium sp. in Chick Gastrointestinal Tract]]>SeidaviA.R. MirhosseiniS.Z. ShivazadM. ChamaniM. SadeghiA.A. 4200834In the present study, a multiplex Polymerase Chain Reaction (mPCR)-based
assay was developed for the simultaneous identification of Salmonella
sp., Escherichia coli and Bifidobacterium sp., that isolated
from broiler gastrointestinal in Iran. In order to detect these bacteria
species, we were used a set of specific primers that produce different
fragment size. Our results showed that all tested gastrointestinal segments
were contaminated by these species and therefore the mPCR method is a
appropriate method to identify these species directly from poultry gastrointestinal
samples. There was no difference in the sensitivity of this method between
samples of different gastrointestinal segments. This method has the potential
to save considerable time and effort within the laboratory. Our developed
mPCR is fast, sensitive, specific and can be used as a reliable choice
for routine detection method of these bacteria species in chick gastrointestinal
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