Asian Journal of Animal Sciences1819-1878xxxx-xxxxxAsian Network for Scientific Information10.3923/ajas.2017.40.46Bos sondaicus×Bos indicus)]]>HartatikTety 12017111Background and Objective: The melanocortin-1-receptor (MC1R) gene is an important candidate gene for the coat color trait. Since, Indonesia has the diversity of local cattle with various type of colour, it is very interest to investigate the sequence of MC1R gene. The aim of this study was to identify polymorphism of melanocortin-1-receptor gene in Indonesian cattle based on sequence analysis. This study was conducted at Laboratory of Animal Genetic and Breeding, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, between August, 2012 and December, 2016. Materials and Methods: The total number of 164 cattle were used in this study that consist of Aceh cattle (n = 25), Filial Ongole cattle (n = 6), Limousin×Madura (Limura, n = 11), Madura cattle (n = 7), Brahman cattle (n = 60), Pesisir cattle (n = 9) and Bali cattle from Kupang (n = 46). A fragment of 296 base pair of melanocortin 1 receptor (MC1R) gene was amplified by forward primer: 5-GGA CCC TGA GAG CAA GCA C-3, reverse primer: 5-CTC ACC TTC AGG GAT GGT CTA-3. Polymerase Chain Reaction (PCR) products were digested with MspI enzyme for identifying of restriction pattern (PCR-RFLP result). Sequencing of the representative sample was conducted base on PCR-RFLP result with different type of polymorphism. Results: The PCR-RFLP analysis showed three pattern of restriction, there are EE genotype, Ee genotype and undigested sample with ee genotype. Sequence analysis show point mutation along the 296 bp of MC1R gene with two type of mutation (substitution 147C→T and deletion/insertion 162indelG). Bali cattle (Bos soandaicus ) and Aceh cattle (Bos indicus) revealed the monomorphic with EE genotype. The others (PO, Limura, Madura, Pesisir and Brahman) showed the polymorphic with equilibrium in chi-square analysis except Limura (Bos soandaicus×Bos indicus×Bos taurus ) with χ2 = 7.64 for Limura population. Conclusion: Local cattle such as Bali and Aceh cattle have higher E allele distribution compare to that of the other local cattle. Indeed, the homozygosity of MC1R gene (EE) in local cattle also higher than that of the crossbred cattle. The MC1R gene can be used to identify the genetic variation of local cattle and showed heterogeneity in crossed breed cattle.]]>Werth, L.A., G.A. Hawkins, A. Eggen, E. Petit, C. Elduque, B. Kreigesmann and M.D. Bishop,199674262262Wang, N. and D.N. Hebert,200619318Fontanesi, L., F. Beretti, V. Riggio, S. Dall'Olio and E.G. Gonzalez et al.,2009MC1R) gene of different goat breeds: Association with red and black coat colour phenotypes but with unexpected evidences.]]>2009Xi, D., M. Wu, Y. Fan, Y. Huo and J. Leng et al.,2012MC1R) in the Chinese yakow (Bos grunniens × Bos taurus).]]>498259263Sasazaki, S., M. Usui, H. Mannen, C. Hiura and S. Tsuji,200576129132Gan, H.Y., J.B. Li, H.M. Wang, Y.D. Gao, W.H. Liu, J.P. Li and J.F. Zhoung,2007TYR and MC1R in Chinese native cattle.]]>78484488Tabun, A.C., T. Hartatik and S. Sumadi,2013MC1R) gene based on coat color of Bali cows of Kupang by using the PCR-RFLP method.]]>388691Li, J., J. Zhao, L. Xu, L. Zhou, X. Li and J. Wang,2008Paris polyphylla var. yunnanensis.]]>24733737Hofreiter, M. and T. Schoneberg,20106725912603Klungland, H. and D.I. Vage,2003994331338Berryere, T.G., S.M. Schmutz., R.J. Schimpf, C.M. Cowan and J. Potter,200334169175Fontanesi, L., F. Beretti, S. Dall'Olio, B. Portolano, D. Matassino and V. Russo,2011MC1R) gene polymorphism is useful for authentication of Massese sheep dairy products.]]>78122128Guastella, A.M., S. Sorbolini, A. Zuccaro, E. Pintus, S. Bordonaro, D. Marletta and N.P.P. Macciotta,2011MC1R) gene polymorphisms in three Italian cattle breeds.]]>5110391043Maudet, C. and P. Taberlet,2002MC1R) gene polymorphism.]]>85707715Klungland, H. and D.I. Vage, 20001223242Rouzaud, F., J. Martin, P.F. Gallet, D. Delourme and V. Goulemot-Leger et al.,200032511520Russo, V., L. Fontanesi, E. Scotti, M. Tazzoli, S. Dall'Olio and R. Davoli,2007MC1R) gene polymorphisms in some cattle breeds: Their usefulness and application for breed traceability and authentication of Parmigiano Reggiano cheese.]]>6257272