Asian Journal of Animal Sciences1819-1878xxxx-xxxxxAsian Network for Scientific Information10.3923/ajas.2015.37.44ZodinsangaV. MaviP.S. CheemaRanjna S. KumarAjeet GandotraV.K. 1201591Success of artificial insemination in bovine industry depends upon quality
of frozen-thawed semen. Therefore, proper analysis of the post-thaw semen can
provide insights into the fertilizing capacity of the cryopreserved semen. Frozen-thawed
semen of 14 cross-bred and 12 pure-bred bulls was analyzed for capacitation/acrosome
reaction and cervical mucus penetration test. Correlations between routine tests/sperm
function and fertility tests were calculated. Mean values for individual motility,
viability, abnormalities, hypo-osmotic swelling test, acrosome integrity and
capacitated/acrosome reacted spermatozoa in frozen-thawed semen were 41.5±1.1,
59.9±1.9, 19.3±1.1, 32.6±0.8, 79.9±3.8, 33.7±2.3
and 40.3±1.1, 76.8±2.5, 17±1.1, 48.4±3.5, 92.9±2.4
and 32.9±2.8% in cross-bred and pure-bred bulls, respectively. It indicated
low mean values of percent viability, acrosome integrity and distance covered
by spermatozoa/30 min in cervical mucus in pure-bred as compared to cross-bred
bulls. Viability and hypo-osmotic swelling test showed an average correlation
with acrosome reacted spermatozoa (+0.58, +0.47). However, there was very weak
correlation between acrosome reaction and viability (+0.06)/hypo-osmotic swelling
test (+0.11)/acrosome integrity (+0.02). Sperm abnormalities were negatively
correlated with acrosome reaction. Weak positive correlation was obtained between
number of spermatozoa penetrated in peak 0.5 cm of cervical mucus and motility
(+0.07)/viability (+0.12)/hypo-osmotic swelling test (+0.14). During the present
study 23, 96 and 53.8% of tested bulls exhibited >40% post-thaw motility,
>55% viability and >35% hypo-osmotic swelling test, whereas, only 46.1,
65.8 and 50% bulls were with >35% acrosome reacted spermatozoa, >25 mm
distance covered/30 min and >350 sperms penetrated in peak 0.5 cm in cervical
mucus penetration test respectively. Therefore on the basis of functional and
fertility tests, it can be predicted that semen of about 50% tested bulls was
of good quality. Motility of sperm is required to reach the site of fertilization
and sperm membrane/acrosome integrity are important for adhesion, penetration
and fertilization of sperm, therefore, it is concluded that sperm function and
fertility tests like HOST, CMPT, acrosome integrity and in vitro capacitation/acrosome
reaction are better indicators for assessment of quality of frozen-thawed semen
and should also be performed before selecting breeding bulls.]]>Babu Rao, K., K. Venugopal Naidu, V. Singh, A. Seshagiri Rao and J. Suresh,1999763537Blom, E.,19501176177Christensen, P., B.P. Brockhoff and H. Lehn‐Jensen,199934503507Cormier, N. and J.L. Bailey, 200369177185Correa, J.R. and P.M. Zavos,199442351360Correa, J.R., M.M. Pace and P.M. Zavos,199748721731Dhami, A.J., G. Mohan and K.L. Sahni,1998195558Felipe-Perez, Y.E., M.L. Juarez-Mosqueda, E.O. Hernandez-Gonzalez and J.J. Valencia,20082123130Fiaz, M., R.H. Usmani, M. Abdullah and T. Ahmad,2010307578Galli, A., M. Basetti, D. Balduzzi, M. Martignoni, V. Bornaghi and M. Maffii,199135837844Forero-Gonzalez, R.A., E.C.C. Celeghini, C.F. Raphael, A.F.C. Andrade, F.F. Bressan and R.P. Arruda,201244154159Januskauskas, A. and H. Zilinskas,2002172936Murase, T. and J.W. Braun,1990347380Nagy, S., J. Jansen, E.K. Topper and B.M. Gadella,20036818281835Neild, D., G. Chaves, M. Flores, N. Mora, M. Beconi and A. Aguero,199951721727Ola, B., M. Afnan, S. Papaioannou, K. Sharif, L. Bjorndahl and A. Coomarasamy,20031810371046Padrik, P., T. Hallap, T. Kaart, T. Bulitko and U. Jaakma,201257490497Parrish, J.J., J.L. Susko-Parrish and N.L. First,198941683699Patel, B.R. and G.M. Siddiquee,20136405408Patel, K.V., A.J. Dhami, M.K. Chauhan, A.S. Dave, V.P. Vadodaria and Y.G. Dugwekar,200053284290Perumal, P., A.K. Barik, D.N. Mohanty, R.K. Das and P.C. Mishra,20092009pp: 196Pons-Rejraji, H., J.L. Bailey and P. Leclerc,200921511524Raval, R.J. and A.J. Dhami,20104-53135Sarma, P.V.,199516127127Sharma, M., M. Singh, S. Kapoor and S. Jasial,201222631Singh, S.P., R.K. Pandit and H.B.S. Bhadoria,200070279280Tas, M., S. Bacinoglu, U. Cirit, O.B. Ozdas and K. Ak,20071011827Ward, C.R. and B.T. Storey,1984104287296Zubair, M., L.A. Lodhi, E. Ahmad and M. Ghulam,20131124128