Asian Journal of Animal Sciences1819-1878xxxx-xxxxxAsian Network for Scientific Information10.3923/ajas.2015.266.279ToradFaisal AmerMohammed ShamaaAshraf El-TookhyOmar SabryDina RashedLaila HamidMagdi Abd El MahfouzSoheir GharibDoaa 6201596Cardiomyocytes in the border zone of an old infarct are condemned to die by necrosis and apoptosis caused by a persistent impairment of the coronary vasodilatory reserve even after successful recanalization of coronary artery total occlusion. Therefore, Chronic Total Occlusion (CTO) remains one of the more challenges for coronary interventions with uncertainty regarding procedural success and long term benefits. Twenty dogs were subjected experimentally to total coronary occlusion; half were treated with human Umbilical Cord Blood derived Mesenchymal Stem Cells (hUCB-MSCs). Clinical, electrocardiographic, echocardiographic, histopathological, biochemical and immunohistochemistry assessments were performed for 6 months at different time intervals. Demonstrated improved systolic function after one month following MSCs injection that was manifested by gradual increase of Fractional Shortening (FS%) and Ejection Fraction (EF). Electrocardiography exhibited improvement of ECG pattern with resolving of abnormal changes (elevated ST segment and inverted T) till retaining approximately normal ECG. After hUCB-MSCs myocardial infiltrations, specific genes in cardiac tissue were highly expressed indicating the differentiation of hUCB-MSCs into endothelial cells. The markedly increased vWF-positive cells in the damaged zone, suggested that angiogenesis is induced by promoting VEGFR2 expression at cardiac injury sites. This improved ischemia, thereby promoting body repair. Improved cardiac efficiency was expressed differently through various assessment parameters.]]>Anzalone, R., S. Corrao, M. Lo Iacono, T. Loria and T. Corsello et al.,201322117Barold, S.S., M.D. Falkoff, L.S. Ong and R.A. Heinle,19875403417Boon, J.A.,20112nd Edn.,Pages: 480Pages: 480Buchanan, J.W. and J. Bucheler,1995206194199James, W.B.,200030379393Chang, S.A., E.J. Lee, H.J. Kang, S.Y. Zhang and J.H. Kim et al.,20082619011912Huang, Z., C.Y. Fong, K. Gauthaman, P. Sukumar, M. Choolani and A. Bongso,201111214751485Zhang, C., W. Zhai, Y. Xie, Q. Chen, W. Zhu and X. Sun,2013in vitro.]]>615771582Bajpai, V.K. and S.T. Andreadis,201218405425Delacretaz, E. and B. Meier,199779185187Dhingra, S., P. Li, X.P. Huang, J. Guo and J. Wu et al.,2013128S69S78Di Nicola, M., C. Carlo-Stella, M. Magni, M. Milanesi and P.D. Longoni et al.,20029938383843Friedenstein, A.J., U.F. Gorskaja and M.N. Kulagina,19764267274Guth, B.D., F.C. White, K.P. Gallagher and C.M. Bloor,1984107458464Han, Y.L., S.L. Wang, Q.M. Jing, Y. Li, J. Zhang, Y.Y. Ma and B. Luan,200611911651170Hua, J., P. Qiu, H. Zhu, H. Cao, F. Wang and W. Li,20115113123Huang, X.P., Z. Sun, Y. Miyagi, H.K. McDonald, L. Zhang, R.D. Weisel and R.K. Li,201012224192429Jiang, Y., B.N. Jahagirdar, R.L. Reinhardt, R.E. Schwartz and C.D. Keene et al.,20024184149Kang, J.W., K.S. Kang, H.C. Koo, J.R. Park, E.W. Choi and Y.H. Park,200817681694Kern, S., H. Eichler, J. Stoeve, H. Kluter and K. Bieback,20062412941301Kogler, G., S. Sensken, J.A. Airey, T. Trapp and M. Muschen et al.,2004200123135Laitinen, A. and J. Laine,20072007Lee, O.K., T.K. Kuo, W.M. Chen, K.D. Lee, S.L. Hsieh and T.H. Chen,20041031669 1675Liechty, K.W., T.C. Mackenzie A.F. Shaaban, A. Radu and A.B. Moseley et al.,2000612821286Oudega, M.,2012349269288Pandian, N.G., R.A. Kieso and R.E. Kerber,198266603611Pham, P.V., N.B. Vu, V.M. Pham, N.H. Tuong and T.L. Pham et al.,20142014Pittenger, M.F., A.M. Mackay, S.C. Beck, R.K. Jaiswal and R. Douglas et al.,1999284143147Poncelet, A.J., J. Vercruysse, A. Saliez and P. Gianello,2007in vitro, intracardiac injection elicits an immune response in vivo.]]>83783790Prasad, A., C.S. Rihal, R.J. Lennon, H.J. Wiste, M. Singh and D.R. Holmes,20074916111618Sabia, P., A. Afrookteh, D.A. Touchstone, M.W. Keller, L. Esquivel and S. Kaul,1991848591Sgarbossa, E.B., S.L. Pinski, A. Barbagelata, D.A. Underwood and K.B. Gates et al.,1996334481487Thomas, W.P., C.E. Gaber, G.J. Jacobs, P.M. Kaplan, C.W. Lombard, N.S. Moise and B.L. Moses,19937247252Torad, F.A. and E.A. Hassan,201416237244Torry, R.J., J.H. Myers, A.L. Adler, C.L. Liut and K.P. Gallagher,199112212921299Uccelli, A., L. Moretta and V. Pistoia,20063625662573Wagner, W., F. Wein, A. Seckinger, M. Frankhauser and U. Wirkner et al.,20053314021416Wijns, W., S.F. Vatner and P.G. Camici,1998339173181