Asian Journal of Agricultural Research1819-1894xxxx-xxxxKnowledgia Review10.3923/ajar.2016.65.71RawarEllsyaAngeline RohmanAbdul 12016101A Flame-Atomic Absorption Spectrophotometry (AAS-flame) for the quantification of cadmium (Cd) and lead (Pb) and mercury analyzer for quantitative analysis of mercury (Hg) in red chili pepper (Capsicum annum L.) were developed and validated. The method was validated for some parameters, namely linearity and range, precision, accuracy, Limit of Detection (LoD) and Limit of Quantification (LoQ), according to International Conference on Harmonization (ICH). The standard curves were linear over the concentration ranges of 0.1-1.4 μg mL1 (Cd), 0.10-1.80 μg mL1 (Pb) and 0.5-20 μg mL1 with correlation coefficient (r) values >0.99. The detection limits obtained were 0.022 μg mL1, 0.0594 μg mL1 and 1.77 μg mL1 for Cd, Pb and Hg, respectively. The quantification limits obtained were 0.075 μg mL1 (Cd), 0.1981 μg mL1 (Cd) and 5.91 μg mL1 (Hg). The Relative Standard Deviation (RSD) values found for its intra-day and interday precision were lower than those required by RSD Horwitz. The mean recovery percentage was 97.98-101.9133%.The validated methods were successfully used for determination of Cd, Pb and Hg in red chili pepper.]]>Ademoroti, C.M.A.,19961st Edn.,Andrews, J.,1995Pages: 186Pages: 186Caldas, E.D. and L.L. Machado,200442599603Ertasa, O.S. and H. Tezel,200536893897EURACHEM,1998Froes, R.E.S., W.B. Neto, N.O.C. Silva, R.L.P. Naveira, C.C. Nascentes and J.B.B. da Silva,200964619622Gonzalez, A.G. and M.A. Herrador,200726227238Garcia-Rico, G., J. Leyva-Perez and M.E. Jara-Marini,20074515991605Govindarajan, V.S. and U.J. Salzer,198623207288Hashem, E.Y.,20025814011410ICH.,19941994Ivanoviene, L., I. Staneviciene, V. Lesauskaite and I. Sadauskiene,200421180184McKinney, J. and R. Rogers,19922612981299Li, J., Y. Zhang, W. Cai and X. Shao,201184679683Millan, R., R. Gamarra, T. Schmid, M.J. Sierra and A.J. Quejido et al.,20063687987Nordberg, G.F., T. Kjellstrom and M. Nordberg,19851985pp: 103-178pp: 103-178Oehme, F.W.,19781st Edn.,Palma, R.J. and K.H. Pearson,197049497504Rahimi, E., M. Hajisalehi, H.R. Kazemeini, A. Chakeri and A. Khodabakhsh et al.,2010949384941Sharma, R.K., M. Agrawal and F.M. Marshall,200766258266Shimoda, R., T. Nagamine, H. Takagi, M. Mori and M.P. Waalkes,200164208215Simonne, A.H., E.H. Simonne, R.R. Eitenmiller, H.A. Mills and N.R. Green,1997Capsicum annuum L.).]]>10299311Taverniers, I., M. De Loose and E. Van Bockstaele,200423535552Tong, S., Y.E. von Schirnding and T. Prapamontol,20007810681077Vogel, A.I.,20015th Edn.,pp: 80pp: 80Voegborlo, R.B. and H. Akagi,2007100853858Wong, S.C., X.D. Li, G. Zhang, S.H. Qi and Y.S. Min,20021193344Waisberg, M., P. Joseph, B. Hale and D. Beyersmann,200319295117