Asian Journal of Applied Sciences1996-3343xxxx-xxxxKnowledgia Review10.3923/ajaps.2019.173.179Scylla tranquebarica]]>FatihahSiti Nor Ab HalikNur Ain Suhaila Muhd-FaroukHarman 42019124Background and Objective: Mud crab, Scylla tranquebarica has been reported to be present in Sabah coastal waters and this specie is one of commercially important crustaceans in the trade markets as demand that has been reported to be increased every year. However, high demands had caused exploitation to this species in Asia and there is still lack of in-depth study about this species mainly on sperm quantity and sexual maturity size. The present study was carried out to investigate the sperm quantity and sex maturation stages based on histology examination. Materials and Methods: Twenty of S. tranquebarica’s male were dissected and the testes were dissected out and divided into two parts where ¾ was used for determination of sperm quantity and ¼ was used for histological examination. The sperm were observed by using microscope under 40x magnification and the quantity were count by using haemacytometer. For histological observation, the testes were fixed in Bouin’s solution for less than 24 h, dehydrated pass through a series of increasing ethanol concentration, cleared with xylene, immersed in paraffin and formed a tissue block. The samples were stained by pass through a series of xylene, decreasing ethanol concentration, hematoxylin and Eosin Y. Results: There was a significant difference between Body Weight (BW), Carapace Width (CW) and sperm quantity with range of body weight for male S. tranquebarica. The highest sperm quantity was recorded on S. tranquebarica with the body weight; (401-500 g). Conclusion: The study concluded that the maturity size of S. tranquebarica was at 246.04 g in BW and 10.6 cm in CW.]]>Keenan, C.P., P.J.F. Davie and D.L. Mann,199846217245Azra, M.N. and M. Ikhwanuddin,2016Scylla broodstock: Present research, problems and future perspective.]]>23257267Fatihah, S.N., S.C. Simon, H.H. Hashim, H. Muhd-Farouk and C.A. Chen,2018Scylla tranquebarica in different cryoprotectants.]]>1110981106Fatihah, S.N., H.T. Julin and C.A. Chen,2017Scylla tranquebarica juveniles at different shelter conditions.]]>1015811589Waiho, K., M. Mustaqim, H. Fazhan, W.I.W. Norfaizza, F.H. Megat and M. Ikhwanuddin,2015Scylla olivacea: The effect of sex ratio and stocking density on mating success.]]>25057Ikhwanuddin, M., S.S. Lan, N. Abdul Hamid, S.N. Fatihah Zakaria, M.N. Azra, A.S. Aisah and A.B. Abol-Munafi,201514885895Phelan, M. and M. Grubert,20072007Poovachiranon, S.,1992Scylla senate (Forskal) in the Mangrove Ecosystem in the Andaman sea.]]>1992pp: 49-57pp: 49-57Ikhwanuddin, M., H. Muhd-Farouk, A.J. Memon, W. Wendy and A.B. Abol-Munafi,2014Scylla olivacea (Herbst, 1796).]]>1710691073Waiho, K., H. Fazhan, S. Jasmani and M. Ikhwanuddin,2017Scylla olivacea (Herbst, 1796)(Decapoda, Brachyura, Portunidae).]]>90119Ikhwanuddin, M., G. Azmie, S.F. Nahar, W. Wee, M.N. Azra and A.B. Abol-Munafi,2018Scylla olivacea) broodstock on different diets.]]>47427432Islam, S. and H. Kurokura,2012Scylla olivacea in a tropical mangrove swamps.]]>7194204Somerton, D.A.,19803714881494Leal, M.C., E.R. Cardoso, R.H. Nobrega, S.R. Batlouni, J. Bogerd, L.R. Franca and R.W. Schulz,200981177187Quinitio, E.T. and F.D. Parado-Estepa,2003Scylla spp.]]>2003Knuckey, I.A.,1996Scylla serrata and the use of mating scars as a functional indicator.]]>16487495Jackson, K., A. Hurvitz, S.Y. Din, D. Goldberg, O. Pearlson, G. Degani and B. Levavi-Sivan,2006Acipenser gueldenstaedtii) with intersex gonads.]]>148359367Fatihah, S.N., H. Muhd-Farouk, A. Amin-Safwan, H.H. Mahsol and M. Ikhwanuddin,2017Panulirus polyphagus (Herbst, 1793).]]>20365371Sato, T., K. Yoseda and T. Shibuno,200824663668Robertson, W.D. and A. Kruger,1994Scylla serrata (Forskal) in Natal, South Africa.]]>39185200Islam, M.S. and M. Kurokura,2013Scylla paramamosain (Forsskal, 1755) in a tropical mangrove swamp.]]>1713