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Biotechnology

Year: 2004 | Volume: 3 | Issue: 1 | Page No.: 75-81
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Research Article

Performance of Transgenic Bt Cotton Against Insect Pest Infestation

G.H. Abro, T.S. Syed, G.M. Tunio and M.A. Khuhro

ABSTRACT

Studies were carried out to investigate insect infestation on transgenic Bt and non-Bt cotton varieties and genotypes. The seeds of seven cotton varieties, viz., KMG-1, KMG-2, KMG-3, MS-1, MS-2 NIAB-78 and CRIS-134 were sown on May 15, 2002 in a completely randomized block design. The study comprised laboratory bioassay and field screening of different varieties and genotypes. Laboratory study indicated that transgenic Bt cotton was highly toxic to Earias vittella causing 100% larval mortality. While armyworm, Spodoptera exigua was tolerant, its mortality ranged between 13.3 to 53.3% on different varieties and genotypes. The results of field study revealed that the highest mean population (1.66 insect per leaf) of jassid was recorded on KMG-3 followed by 1.95 and 1.52 insects leaf on KMG-2 and KMG-1, respectively. Whitefly population ranged between 1.47 and 1.52 insects leaf on different varieties and genotypes. Whereas, thrips population remained fluctuating in different varieties and genotypes during the crop growth period. But genotypes like KMG-1, KMG-2 and KMG-3 which are crosses of Bt cotton and non-Bt cotton varieties were found harboring more thrips population. The larval population of spotted bollworm, Earias spp. indicated no infestation and live larvae were found on MS-1 and MS-2 varieties of Bt cotton. Maximum infestation of spotted bollworm was recorded on NIAB-78 (0.97 larva per plant) followed by other varieties and genotypes.
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REFERENCES

  1. Barton, K.A., H.R. Whiteley and N.S. Yang, 1987. Bacillus thuringiensis, delta endotoxin expressed in Nicotiana tabacum provides resistance to Lepidoptera insects. Plant Physiol., 85: 1103-1109.
  2. Fischhoff, D.A., K.S. Bowdish, F.J. Perlak, P.G. Marrone and S.M. McCormick et al., 1987. Insect tolerant transgenic tomato plants. Nat. Biotechnol., 5: 807-813.
    CrossRefDirect Link
  3. Vaeck, M., A. Reynearts, H. Hofte, S. Jansens and M. DeBeuckleer et al., 1987. Transgenic plants protected from insect attack. Nature, 328: 33-37.
    CrossRefDirect Link
  4. Perlak, F.J., R.L. Fuchs, D.A. Deans, S.L. McPherson and D.A. Fischhoff, 1991. Modification of the coding sequence enhance plant expressing of insect control protein genes. Proc. Natl. Acad. Sci. USA., 88: 3324-3328.
    Direct Link
  5. Gill, S.S., E.A. Cowles and P.V. Pietrantonio, 1992. The mode of action of Bacillus thuringiensis endotoxins. Annu. Rev. Entomol., 37: 615-636.
  6. Dulmage, H.T., H.M. Graham and E. Martinez, 1978. Interaction between the tobacco budworm, Heliothis virescens and the δ-endotoxin produced by the HD-1 isolate the Bacillus thuringiensis var. kurstaki. Relationship between length of exposure to the toxin and survival. J. Invertebr. Pathol., 32: 40-50.
  7. Halcomb, K.L., J.H. Benedict, B. Cook and D.R. Ring, 1996. Survival and growth of bollworm and tobacco budworm on nontransgenic and transgenic cotton expressing a cry IA insecticidal protein (Lepidoptera: Noctuidae). Environ. Entomol., 25: 250-255.
    Direct Link
  8. Benedict, J.H., E.S. Achs, D.W. Altman, D.R. Riing, T.B. Stone and S.R. Sims, 1993. Impact of delta endotoxin producing transgenic cotton on insect-plant interactions with cotton on insert plant interactions with Heliothis virescens and Helicoverpa zea (Lepidoptera: Noctuidae). Environ. Entomol., 22: 1-9.
  9. Ring, D.R., J.H. Benedict, D.J. Lawlor, R.R. Despain and T. Stone, 1993. Bollworm and tobacco budworm injury to transgenic Bt cotton and estimated density-yield responses using the integrated crop ecosystem management model. Proceedings of the Beltwide Cotton Product Conference National Cotton Council of America, (BCPCNCCA`93), Memphis, TN., USA., pp: 821-824.
  10. Mahaffey, J.S., J.S. Bacheler, J.R.Jr. Bradley and J.W. Van Duyn, 1994. Performance of Monsantos transgenic Bt cotton against high population of lepidopterous pests in North Carolina. Proceedings of the Beltwide Coton Conference National Cotton Council of America, (BCCNCCA`94), Mamphis TN., pp: 1061-1063.
  11. Olsen, K.M. and J.C. Daly, 2000. Plant-toxin interactions in transgenic Bt cotton and their effect on mortality of Helicoverpa armigera (Lepidoptera: Noctuidae). J. Econ. Entomol., 93: 1293-1299.
    Direct Link
  12. Zhou, Z.J., Z.K. Jun, F.X. Lin, L.M. Guang, R.C. Hui, Z.H. Zhen and G.S. Dui, 2000. Comparison of insecticidal activity of Bt cotton lines both developed in China and USA. against Helicoverpa armigera. Scientia Agric. Sinica, 33: 100-102.
  13. Camberos, U.N., M. Berdegue, H.S. Galven, E.R.G. Rodriguez and P. Dugger, 1999. Monitoring of pinkbollworm susceptibility to the Bt endotoxin (Cry 1AC) in the Camcara laqunera, Mexico. Proc. Beltwide Cotton Conf. Orlando, Florida, USA., 2: 1246-1248.
  14. Patin, A.L., T.L. Dennehy, M.A. Sims, B.E. Tabashnik and L.Y. Biao et al., 1999. Status of pink bollworm susceptibility to Bt in Arizona. Proc. Beltwide Cotton Conf. Olando, Florida, USA., 2: 991-996.
  15. Ahmed, Z., 1980. Incidence of major cotton pests and diseases in Pakistan with special reference to pest management. Proceedings of the International Consultation on Cotton Production Research with Focus on the Asian Region, November 17-21, 1980, Manila, Philippines, pp: 156-179.
  16. Steel, R.G.D. and J.H. Torrie, 1980. Principles and Procedures of Statistics: A Biometrical Approach. 2nd Edn., McGraw Hill Book Co., New York, USA., ISBN-13: 9780070609266, Pages: 633.
    Direct Link
  17. Ashfaq, M.S.Y. Young and P. Dugger, 1999. Effect of transgenic Bt cotton on larval mortality and development of beet armyworm, Spodotera exigua (Lepidoptera: Noctuidae). Proc. Beltwisd Cotton Conf. Orlando, USA., 2: 1232-1234.
  18. Henneberry, T.J., L.F. Tech, T. Delatorre and T. Delaltorre, 2001. Effect of transgenic cotton on on cabbage looper, tobacco budworm and beet armyworm (Lepidoptera: Noctuidae) Larval mortality and development and foliage consumption in the laboratory. Southwestern Entomol., 26: 325-338.
  19. Adamczyk, J.J., D.D. Hardee, L.C. Adams and D.V. Sumerford, 2001. Correlating differences in larval survival and development of bollworm (Lepidoptera: Noctuidae) and fall armyworm (Lepidoptera Noctuidae) to differential expression of Cry1A(c) delta-endotoxin in various plant parts among commercial cultivars of transgenic Bacillus thuringiensis cotton. J. Econ. Entomol., 94: 284-290.
    Direct Link
  20. Adamczyk, J.J. and D.V. Sumerford, 2001. Increased tolerance of fall armyworms (Lepidoptera: Noctuidae) to Cry1Ac delta-endotoxin when fed transgenic Bacillus thuringiensis cotton impact on the development of subsequent generations. Florida Entomol., 84: 1-6.
  21. Ponsard, S., A.P. Gutierrez and N.J. Mills, 2002. Effect of Bt-toxin (Cry1Ac) in transgenic cotton on the adult longevity of four heteropteran predators. Environ. Entomol., 31: 1197-1205.
    Direct Link
  22. Deng, S., J. Xu, Q. Zhang, S. Zhou and G. Xu, 2003. Effect of transgenic Bt cotton on population dynamics of the non target pests and natural enemies of pests. Acta Entomol. Sinica, 46: 1-5.
    PubMedDirect Link
  23. Hendrix, D.L., 1995. The relationship between whitefly populations honeydew deposition and stickiness in cotton lint. Proc. Beltwide Cotton Conf., 1: 104-105.
  24. Taley, Y.M., R.I. Thote and S.A. Nimbalkar, 1988. Assessment of crop losses due to insect pests of cotton and cost benefit of protection schedule. PKV. Res. J., 12: 126-128.
  25. Singh, J.P. and R.K. Lakra, 1992. Effect of incidence of leafhopper and bollworms on shedding of fruiting bodies and loss in yield of seed cotton. Proc. 3rd Int. Conf. P. Prot., 6: 142-148.
  26. Baloch, A.A., R.A. Soomro, M.A. Leghari and M.W. Sanjrani, 1990. Studies on economic injury levels of insect pests of cotton. Turk. Entomol. Dergisi, 14: 131-148.
  27. Zhu, N.X., S.Q. Ping, K.X. Hui, C. Hong, M.J. Wen, H.Y. Jiang and Z.S. Lin, 2001. A preliminary research on the regularity of population fluctuations of major insects and natural enemies in the field of Bt transgenic cotton in the Xinjiang region. China Cotton, 28: 12-13.
  28. Xin, B.L., Z.L. Wa, C.X. Bo, F.H. Jin, G.G. Hua, Z.Z. Qing and S. Chun'e, 2002. Preliminary studies on effects of transgenic cotton varieties on composition of insect community. Chinese J. Biol. Con., 18: 115-119.
  29. Gui, S.C., X. Jing, Z.Q. Wen, F.H. Bing, W. Fei and S. Rong, 2002. Effect of transgenic Bt cotton on population of cotton pests and their natural enemies in Xinjiang. Chinese J. Biol. Cont., 18: 106-110.
  30. Butter, N.S., J.S. Kular and T.H. Singh, 1994. Action threshold studies of bollworms for pesticidal application in cotton. Plant-Prot.-Bull.-Faridabad, 46: 7-11.
  31. Chen, S., Z.D. Sheng, W.Z. Ting, W.X. Lin and Zhopu, 2002. Changes of resistance of Bt transgenic cotton 32B at different growth stages and its physiological mechanisms. Jiangsu J. Agric., 18: 84-84.
  32. Xia, L.X., W. Fei, X. Jing, Z.Q. Wen, F.H. Bing and S. Rong, 2002. Transgenic Bt cotton's expression on cotton bollworm's resistance and effect on the arthropod community in the field of South Xinjiang. J. China Agric. Univ., 7: 70-74.

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