J. Zouine
Department of Biology, Faculty of Science Semlalia, Maroc
I. El Hadrami
Department of Biology, Faculty of Science Semlalia, Maroc
ABSTRACT
The present study was conducted to determine the effect of three concentrations of usually used (1.5, 3 and 6%) of sucrose on the proliferation of date palm cell suspension culture. The medium containing 3% sucrose, leads to best results than 1.5 and 6% sucrose especially for JHL cultivar. In fact, when sucrose concentration increased from 1.5 to 3%, the embryogenic mass significantly increased from 2.15 to 14.6 g per flask and 8.7 to 10 g per flask, respectively for JHL and BSTN cultivars. The biochemical analysis showed that the maturation medium with 3% sucrose significantly increased the proteins (14.9 mg g-1 FW for JHL cultivar and 5 mg g-1 FW for BSTN cultivar) and sugars contents (22.5 and 22.7 mg g-1 FW, respectively, for JHL and BSTN cultivars) in the embryogenic mass. In addition, as compared to 1.5 and 6%, the use of 3% sucrose enhanced phenolics contents (0.69 mg g-1FW) and peroxidases activities (2.9.103 n Kat g-1FW) in BSTN cultivar.
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How to cite this article
J. Zouine and I. El Hadrami, 2004. Somatic Embryogenesis in Phoenix dactylifera L: Effect of Exogenous
Supply of Sucrose on Proteins, Sugars, Phenolics and Peroxidases Activities
During the Embryogenic Cell Suspension Culture. Biotechnology, 3: 114-118.
DOI: 10.3923/biotech.2004.114.118
URL: https://scialert.net/abstract/?doi=biotech.2004.114.118
DOI: 10.3923/biotech.2004.114.118
URL: https://scialert.net/abstract/?doi=biotech.2004.114.118
REFERENCES
- Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
CrossRefDirect Link - Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
CrossRefPubMedDirect Link - Cvikrova, M., P. Binarova, J. Eder, M. Vagner, M. Hrubcova, J. Zon and I. Machackova, 1999. Effect of inhibition of phenylalanine ammonia-Lyase activity on growth of alfalfa cell suspension culture: Alterations in mitotic index, ethylene production and contents of phenolic, cytokinins and polyamines. Physiol. Plant., 107: 329-337.
- DuBois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith, 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem., 28: 350-356.
CrossRefDirect Link