Effectivity of Immunoglobulin Y Anti Lipase as a Pancreatic Lipase Inhibitor for Prevention of Obesity*
Abstract:
Obesity has been a world-wide health problem and associated with many degenerative diseases. Two approved anti-obesity compounds, sibutramine and orlistat, have various serious side effects which limit their uses. IgY anti lipase will inhibit the activity of pancreatic lipase in the gastrointestinal tract. The objective of this study was to evaluate the effectivity of IgY anti lipase obtained from yolk as pancreatic lipase inhibitor for prevention of obesity. IgY anti lipase was obtained from the yolk of hens immunized with porcine pancreatic lipase and was purified with sodium sulphate precipitation. The protein was analyzed by SDS-PAGE and was identified by Immunoblotting. The ability of IgY anti lipase to inhibit pancreatic lipase was tested by ELISA colorimetric microplate assay at five grades of concentration. Yolk containing IgY anti lipase was then tested in rabbit to evaluate its ability to inhibit fat absorption in vivo. The result showed that IgY contained two proteins with molecular weights of 61.2 and 26.9 kDa and was positively reacted with the rabbit anti chicken coupled with HRP. IgY anti lipase and orlistat did not differ in inhibition capacity at various concentrations (p>0.05). The inhibition capacity of IgY obtained from unimmunized hen was significantly lower than IgY anti lipase and orlistat (p<0.05). Yolk containing IgY anti lipase also had the ability to inhibit dietary fat absorption in vivo, but its inhibition capacity was lower than orlistat (p<0.05). It is concluded that IgY anti lipase could be used to inhibit fat absorption to prevent obesity.
How to cite this article
Ronald Tarigan, Nastiti Kusumorini and Wasmen Manalu, 2016. Effectivity of Immunoglobulin Y Anti Lipase as a Pancreatic Lipase Inhibitor for Prevention of Obesity*. Pakistan Journal of Nutrition, 15: 625-632.
REFERENCES
Akita, E.M. and S. Nakai, 1992. Immunoglobulins from egg yolk: Isolation and purification. J. Food Sci., 57: 629-634.
CrossRef Direct Link
Badmaev, V., Y. Hatakeyama, N. Yamazaki, A. Noro, F. Mohamed, C.T. Ho and M.H. Pan, 2015. Preclinical and clinical effects of Coleus forskohlii, Salacia reticulata and Sesamum indicum modifying pancreatic lipase inhibition in vitro and reducing total body fat. J. Funct. Foods, 15: 44-51.
CrossRef Direct Link
Birari, R.B. and K.K. Bhutani, 2007. Pancreatic lipase inhibitors from natural sources: Unexplored potential. Drug Discovery Today, 12: 879-889.
CrossRef
Blum, P.M., D.L. Phelps, B.J. Ank, H.J. Krantman and E.R. Stiehm, 1981. Survival of oral human immune serum globulin in the gastrointestinal tract of low birth weight infants. Pediatr. Res., 15: 1256-1260.
CrossRef Direct Link
Carlender, D., 2002. Avian IgY antibody in vitro and in vivo. Ph.D. Thesis, Universitatis Uppsala, Uppsala, Sweden.
Carriere, F., P. Grandval, P.C. Gregory, C. Renou, F. Henniges, S. Sander-Struckmeier and R. Laugier, 2005. Does the pancreas really produce much more lipase than required for fat digestion? JOP J. Pancreas, 6: 206-215.
PubMed Direct Link
Choi, S.J., J.M. Hwang and S.I. Kim, 2003. A colorimetric microplate assay method for high throughput analysis of lipase activity. J. Biochem. Mol. Biol., 36: 417-420.
PubMed
Choudhary, M. and K. Grover, 2012. Development of functional food products in relation to obesity. Funct. Foods Health Dis., 2: 188-197.
Direct Link
De Caro, J., M. Boudouard, J. Bonicel, A. Guidoni, P. Desnuelle and M. Rovery, 1981. Porcine pancreatic lipase. Completion of the primary structure. Biochimica et Biophysica Acta (BBA)-Protein Struct., 671: 129-138.
CrossRef Direct Link
De Simone, G. and G. D'Addeo, 2008. Sibutramine: Balancing weight loss benefit and possible cardiovascular risk. Nutr. Metab. Cardiovas. Dis., 18: 334-341.
CrossRef PubMed Direct Link
Dojana, N., I. Condreanu and C. Preda, 2012. Enzym ouput capacity of the rabbit exocrine pancreas to adapt differently to food substrate concentration changes. Proceedings of the 10th World Rabbit Congress, September 3-6, 2012, Egypt, pp: 134-141.
Dujovne, C.A., J.H. Zavoral, E. Rowe and C.M, Mendel, 2001. Effects of sibutramine on body weight and serum lipids: A double-blind, randomized, placebo-controlled study in 322 overweight and obese patients with dyslipidemia. Am. Heart J., 142: 489-497.
CrossRef Direct Link
Duncan, D.B., 1955. Multiple range and multiple F tests. Biometrics, 11: 1-42.
CrossRef Direct Link
Eibl, M.M., H.M. Wolf, H. Furnkranz and A. Rosenkranz, 1988. Prevention of necrotizing enterocolitis in low-birth-weight infants by IgA-IgG feeding. N. Engl. J. Med., 319: 1-7.
CrossRef Direct Link
FDA., 2012. Drug safety communication: Completed safety review of Xenical/Alli (orlistat) and severe liver injury. http://www.fda.gov/Drugs/DrugSafety/PostmarketDrugSafetyInformationforPatientsandProviders/ucm213038.htm.
Aleman-Gomez, J. A., N.S. Colwell, T. Sasser and V.B. Kumar, 1992. Molecular cloning and characterization of rabbit pancreatic triglyceride lipase. Biochem. Biophys. Res. Commun., 188: 964-971.
CrossRef Direct Link
Hadvary, P., W. Sidler, W. Meister, W. Vetter and H. Wolfer, 1991. The lipase inhibitor tetrahydrolipstatin binds covalently to the putative active site serine of pancreatic lipase. J. Biochem., 266: 2021-2027.
Direct Link
Hatta, H., M. Kim and T. Yamamoto, 1990. A novel isolation method for hen egg yolk antibody, IgY. Agric. Biol. Chem., 54: 2531-2535.
CrossRef PubMed Direct Link
Jaradat, Z.W. and R.R. Marquardt, 2000. Studies on the stability of chicken IgY in different sugars, complex carbohydrates and food materials. Food Agric. Immunol., 12: 263-272.
CrossRef Direct Link
Jenesius, J.C. and C. Koch, 1997. Antibodies Packaged in Eggs. In: Immunochemistry: A Practical Approach, Jonstone, A.P. and M.W. Turner (Eds.). 1st Edn., Oxford University Press, Oxford, pp: 69-79
Kazemipoor, M., C.W.J.W.M. Radzi, G.A. Cordell and I. Yaze, 2012. Potential of traditional medicinal plants for treating obesity: A review. Proceedings of the 2012 International Conference on Nutrition and Food Science, IPCBEE Vol. 39, July 23-24, 2012, IACSIT Press, Singapore, pp: 1-6.
Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
CrossRef Direct Link
Larsson, A., D. Carlander and M. Wilhelmsson, 1998. Antibody response in laying hens with small amounts of antigen. Food Agric. Immunol., 10: 29-36.
CrossRef Direct Link
Li, X., T. Nakano, H.H. Sunwoo, B.H. Paek, H.S. Chae and J.S. Sim, 1998. Effects of egg and yolk weights on yolk antibody (IgY) production in laying chickens. Poult. Sci., 77: 266-270.
CrossRef Direct Link
Mo, E.J., Y.H. Jo, J.Y. Jeong, S.B. Kim, Q. Liu, B.H. Hwang and M.K. Lee, 2016. Pancreatic lipase inhibitory phthalide derivatives from the rhizome of Cnidium officinale. Rec. Nat. Prod., 10: 148-153.
Direct Link
Kovacs-Nolan, J. and Y. Mine, 2004. Avian egg antibodies: Basic and potential applications. Avian Poult. Biol. Rev., 15: 25-46.
CrossRef Direct Link
Padwal, R., S.K. Li and D.C.W. Lau, 2003. Long-term pharmacotherapy for overweight and obesity: A systematic review and meta-analysis of randomized controlled trials. Int. J. Obes., 27: 1437-1446.
CrossRef Direct Link
Ryan, D.E. and W. Levin, 1990. Purification and characterization of hepatic microsomal cytochrome P450. Pharmacol. Ther., 45: 153-239.
PubMed
Pimentel, J., 2008. Decreased fat absorption with an anti-lipase antibody. Patent No. US 7344713 B1. http://www.google.com/patents/US7344713.
Rahman, S., S. van Nguyen, F.C. Icatlo Jr., K. Umeda and Y. Kodama, 2013. Oral passive IgY-based immunotherapeutics: A novel solution for prevention and treatment of alimentary tract diseases. Hum. Vaccines Immunotherapeut., 9: 1039-1048.
CrossRef Direct Link
Ramirez, G., M. Zavala, J. Perez and A. Zamilpa, 2012. In vitro screening of medicinal plants used in Mexico as antidiabetics with glucosidase and lipase inhibitory activities. Evidence-Complement. Altern. Med.
CrossRef
Rubio, M.A., M. Gargallo, A.I. Millan and B. Moreno, 2007. Drugs in the treatment of obesity: Sibutramine, orlistat and rimonabant. Public Health Nutr., 10: 1200-1205.
CrossRef Direct Link
Schade, R., D. Pfister, R. Halatsch and P. Henklein, 1991. Polyclonal IgY antibodies from chicken egg yolk: An alternative to the production of mammalian IgG type antibodies in rabbits. ATLA., 19: 403-419.
Tonstad, S., S. Rossner, A. Rissanen and A. Astrup, 2016. Medical management of obesity in Scandinavia 2016. Obes. Med., 1: 38-44.
CrossRef Direct Link
HHS., 2004. Prescription medication for the treatment of obesity. U.S. Department of Health and Human Services (HHS), Washington, DC.
WHO., 2013. Obesity and overweight. Fact Sheet No. 311, World Health Organization, Geneva, Switzerland. http://www.who.int/mediacentre/factsheets/fs311/en/index.html.
WHO., 2003. Diet, nutrition and the prevention of chronic diseases: Report of a joint WHO/FAO expert consultation. WHO Technical Report Series No. 916, World Health Organization, Geneva, Switzerland.
WHO., 2006. Obesity and overweight. Fact Sheet No. 311, World Health Organization, Geneva, September 2006.
Yun, J.W., 2010. Possible anti-obesity therapeutics from nature-A review. Phytochemistry, 71: 1625-1641.
CrossRef Direct Link
Zheng, C.D., Y.Q. Duan, J.M. Gao and Z.G. Ruan, 2010. Screening for anti-lipase properties of 37 traditional Chinese medicinal herbs. J. Chin. Med. Assoc., 73: 319-324.
CrossRef Direct Link
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