LC ESI-MS and FT-IR Analysis of Dendrophthoe pentandra L. Miq Leaf Methanolic Extracts to Identify Compounds with Progesterone-Like Effects
Abstract:
An earlier study found that progesterone hormone levels in adult female rats increased by nearly two-fold relative to usual levels following intramuscular injection (w/v, 100 mg/kg body weight for four days (s.d.d.)) of methanol extracts of Dendrophthoe petandra L. Miq (common name Benalu duku) leaves. This result suggests that Benalu duku contains pregnane derivative steroids that have carbon bonding at positions 1 and 21. Here we examined the specificity and pharmacodynamics of active substances with progesterone-like effects in crude methanol extracts made from Benalu duku leaves. Pulverized Benalu duku leaves (400 g) were extracted with 2 L analytical grade methanol at 20°C for 72 h using a shaker maceration method. The semi-solid crude extract was dried under vacuum and exposed to UV light prior to spectroscopic analysis. FT-IR and LC ESI MS analysis detected active substances having a pregnane derivative chemical structure with progesterone, medroxy progesterone acetate, megestrol acetate and dydrogesterone present at distributions of about 30, 66, 3 and 1%.
How to cite this article
M. Lazuardi and B. Hermanto, 2016. LC ESI-MS and FT-IR Analysis of Dendrophthoe pentandra L. Miq Leaf Methanolic Extracts to Identify Compounds with Progesterone-Like Effects. Pakistan Journal of Nutrition, 15: 274-282.
REFERENCES
Ameer, O.Z., I.M. Salman, K.J. Quek and M.Z. Asmawi, 2015. Loranthus ferrugineus: A mistletoe from traditional uses to laboratory bench. J. Pharmacopunct., 18: 7-18.
Direct Link
Ang, H.Y., T. Subramani, S.K. Yeap, A.R. Omar, W.Y. Ho, M.P. Abdullah and N.B. Alitheen, 2014. Immunomodulatory effects of Potentilla indica and Dendrophthoe pentandra on mice splenocytes and thymocytes. Exp. Ther. Med., 7: 1733-1737.
Direct Link
Arung, E.T., I.W. Kusuma, E.O. Christy, K. Shimizu and R. Kondo, 2009. Evaluation of medicinal plants from Central Kalimantan for antimelanogenesis. J. Nat. Med., 63: 473-480.
CrossRef Direct Link
Arun, L.B., A.M. Arunachalam, K.D. Arunachalam, S.K. Annamalai and K.A. Kumar, 2014. In vivo anti-ulcer, anti-stress, anti-allergic and functional properties of gymnemic acid isolated from Gymnema sylvestre. R. Br. BMC Complement. Alternat. Med., Vol. 14.
Batchu, S.R., N. Quinete, V.R. Panditi and P.R. Gardinali, 2013. Online solid phase extraction liquid chromatography tandem mass spectrometry (SPE-LC-MS/MS) method for the determination of sucralose in reclaimed and drinking waters and its photo degradation in natural waters from South Florida. Chem. Central J., Vol. 7.
Cooper, L.A. and S.T. Page, 2014. Androgens and prostate disease. Asian J. Androl., 16: 248-255.
Direct Link
Del Pup, L., M. Berretta, R. Di Francia, C. Cavaliere and M. Di Napoli et al., 2014. Nomegestrol acetate/estradiol hormonal oral contraceptive and breast cancer risk. Anti-Cancer Drugs, 25: 745-750.
CrossRef Direct Link
Fackler, K., J.S. Stevanic, T. Ters, B. Hinterstoisser, M. Schwanninger and L. Salmen, 2010. Localisation and characterisation of incipient brown-rot decay within spruce wood cell walls using FT-IR imaging microscopy. Enzyme Microbial Technol., 47: 257-267.
CrossRef Direct Link
Yin, J., Y. Xia and M. Lu, 2012. Concentration profiles of collagen and proteoglycan in articular cartilage by Fourier transform infrared imaging and principal component regression. Spectrochimica Acta Part A: Mol. Biomol. Spectroscopy, 88: 90-96.
CrossRef Direct Link
Kind, T. and O. Fiehn, 2010. Advances in structure elucidation of small molecules using mass spectrometry. Bioanal. Rev., 2: 23-60.
Direct Link
Kushnir, M.M., A.L. Rockwood, J. Bergquist, M. Varshavsky and W.L. Roberts et al., 2008. High-sensitivity tandem mass spectrometry assay for serum estrone and estradiol. Am. J. Clin. Pathol., 129: 530-539.
PubMed Direct Link
Kwanda, N., K. Noikotr, R. Sudmoon, T. Tanee and A. Chaveerach, 2013. Medicinal parasitic plants on diverse hosts with their usages and barcodes. J. Natural Med., 67: 438-445.
CrossRef Direct Link
Mochamad, L. and B. Hermanto, 2014. Dendrophthoe pentandra methanolic leaf extract increases progesterone levels in female rats. Univ. Med., 33: 100-108.
CrossRef Direct Link
Mori, G.M., M.I. Zucchi, I. Sampaio and A.P. Souza, 2015. Species distribution and introgressive hybridization of two Avicennia species from the Western Hemisphere unveiled by phylogeographic patterns. BMC Evolut. Biol., Vol. 15.
Ogbuewu, I.P., I.C. Unamba-Oparah, V.U. Odoemenam, I.F. Etuk and I.C. Okoli, 2011. The potentiality of medicinal plants as the source of new contraceptive principles in males. North Am. J. Med. Sci., 3: 255-263.
Direct Link
Ono, M., K. Kashimada, K. Miyai, T. Onishi, M. Takagi, S. Honma and S. Mizutani, 2008. In vitro enzyme assay of CYP21A2 mutation (R483Q) by a novel method using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Clin. Pediatric Endocrinol., 17: 49-56.
Direct Link
Sivils, J., C.L. Storer, M.D. Galigniana and M.B. Cox, 2011. Regulation of steroid hormone receptor function by the 52-kDa FK506-binding protein (FKBP52). Curr. Opin. Pharmacol., 11: 314-319.
CrossRef PubMed Direct Link
Tuli, L. and H.W. Ressom, 2009. LC-MS based detection of differential protein expression. J. Proteomics Bioinform., 2: 416-438.
Direct Link
Li, X. and A.A. Franke, 2011. Improved LC-MS method for the determination of fatty acids in red blood cells by LC-orbitrap MS. Anal. Chem., 83: 3192-3198.
Direct Link
Zhang, S., S.R. Mada, S. Sharma, M. Torch, D. Mattison, S. Caritis and R. Venkataramanan, 2008. Simultaneous quantitation of 17α-hydroxyprogesterone caproate, 17α-hydroxyprogesterone and progesterone in human plasma using high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS). J. Pharmaceut. Biomed. Anal., 48: 1174-1180.
CrossRef Direct Link
© Science Alert. All Rights Reserved