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Pakistan Journal of Nutrition

Year: 2015 | Volume: 14 | Issue: 10 | Page No.: 647-652
DOI: 10.3923/pjn.2015.647.652
Antioxidant Activity of Rice Bran Protein Extract, its Enzymatic Hydrolysates and its Combination with Commercial Antioxidants
Ladda Wattanasiritham, Supatcha Kubglomsong and Chockchai Theerakulkait

Abstract: Rice bran protein extracted from defatted Khao Dawk Mali (KDML 105) rice bran using alkali extraction and isoelectric precipitation (RBPE) was prepared. RBPE was hydrolyzed with Alcalase 2.4 L and antioxidant activity of the hydrolysates were determined by 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and Ferric Reducing Ability Power (FRAP) assay at hydrolysis time of 0, 30, 60, 90 and 120 min. The DPPH free radicals scavenging activity and FRAP value of the alcalase RBPE hydrolysates were 32.1-35.5% and 951-1,018 μmol FeSO4/mL of hydrolysate, respectively and were not significantly different (p>0.05) at different hydrolysis times. RBPE was freeze-dried (FD-RBPE) and it was found that freeze-drying did not affect antioxidant activity of RBPE. DPPH radical scavenging activity and FRAP of the FD-RBPE was 41.9% and 92.6 μmol FeSO4/g protein, respectively. FD-RBPE was combined with butyrate hydroxyanisole (BHA) and their antioxidant activities were evaluated. It showed that FD-RBPE did not affect antioxidant activity of BHA. Based on DPPH radical-scavenging activity, 106 μg protein of FD-RBPE can replace 1 μg of BHA. It suggested that FD-RBPE can replace BHA or be combined with BHA as antioxidant in food products.

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How to cite this article
Ladda Wattanasiritham, Supatcha Kubglomsong and Chockchai Theerakulkait, 2015. Antioxidant Activity of Rice Bran Protein Extract, its Enzymatic Hydrolysates and its Combination with Commercial Antioxidants. Pakistan Journal of Nutrition, 14: 647-652.

Keywords: rice bran protein extract, Antioxidant activity and combination effect

REFERENCES
  • Adebiyi, A.P., A.O. Adebiyi, J. Yamashita, T. Ogawa and K. Muramoto, 2009. Purification and characterization of antioxidative peptides derived from rice bran protein hydrolysates. Eur. Food Res. Technol., 228: 553-563.
    CrossRef    Direct Link    

  • Adebiyi, A.P., A.O. Adebiyi, T. Ogawa and K. Muramot, 2007. Preparation and characterization of high-quality rice bran proteins. J. Sci. Food Agric., 87: 1219-1227.
    CrossRef    Direct Link    

  • AOAC., 1990. Official Methods of Analysis. 16th Edn., AOAC., Washington, DC. USA., Pages: 1298
    Direct Link    

  • Benzie, I.F.F. and J.J. Strain, 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal. Biochem., 239: 70-76.
    CrossRef    PubMed    Direct Link    

  • Hernandez-Ledesma, B., A. Quiros, L. Amigo and I. Recio, 2007. Identification of bioactive peptides after digestion of human milk and infant formula with pepsin and pancreatin. Int. Dairy J., 17: 42-49.
    CrossRef    Direct Link    

  • Bougatef, A., N. Nedjar-Arroume, L. Manni, R. Ravallec, A. Barkia, D. Guillochon and M. Nasri, 2010. Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle (Sardinellaaurita) by-products proteins. Food Chem., 118: 559-565.
    CrossRef    Direct Link    

  • Chanput, W., C. Theerakulkait and S. Nakai, 2009. Antioxidative properties of partially purified barley hordein, rice bran protein fractions and their hydrolysates. J. Cereal Sci., 49: 422-428.
    CrossRef    Direct Link    

  • Gnanasambandam, R. and N.S. Heltiarachchy, 1995. Protein concentrates from unstabilized and stabilized rice bran: Preparation and properties. J. Food Sci., 60: 1066-1069.
    CrossRef    Direct Link    

  • Hamada, J.S., 1997. Characterization of protein fractions of rice bran to devise effective methods of protein solubilization. Cereal Chem., 74: 662-668.
    CrossRef    Direct Link    

  • Ito, N., S. Fukushima and H. Tsuda, 1985. Carcinogenicity and modification of the carcinogenic response by BHA, BHT and other antioxidants. Crit. Rev. Toxicol., 15: 109-150.
    CrossRef    PubMed    Direct Link    

  • Kokkeaw, H. and S. Thawornchinsombut, 2007. Process optimization of jasmine rice bran protein hydrolysates and its radical scavenging property. Agric. Sci. J., 38: 177-180.
    Direct Link    

  • Kim, S.Y., J.Y. Je and S.K. Kim, 2007. Purification and characterization of antioxidant peptide from hoki (Johnius belengerii) frame protein by gastrointestinal digestion. J. Nutr. Biochem., 18: 31-38.
    CrossRef    Direct Link    

  • Xu, L., H.M. Li, Y.B. Huang, H. Wang, G.J. Nie and X.Z. Zhang, 2006. A study on anti-oxidative activity of soybean peptides with linoleic acid peroxidation systems. Chem. Res. Chin. Univ., 22: 205-208.
    CrossRef    Direct Link    

  • Qi, M., N.S. Hettiarachchy and U. Kalapathy, 1997. Solubility and emulsifying properties of soy protein isolates modified by pancreatin. J. Food Sci., 62: 1110-1115.
    CrossRef    Direct Link    

  • Tang, C.H., J. Peng, D.W. Zhen and Z. Chen, 2009. Physicochemical and antioxidant properties of buckwheat (Fagopyrum esculentum Moench) protein hydrolysates. Food Chem., 115: 672-678.
    CrossRef    Direct Link    

  • Wang, J.S., M.M. Zhao, Q.Z. Zhao and Y.M. Jiang, 2007. Antioxidant properties of papain hydrolysates of wheat gluten in different oxidation systems. Food Chem., 101: 1658-1663.
    CrossRef    Direct Link    

  • Willcox, J.K., S.L. Ash and G.L. Catignani, 2004. Antioxidants and prevention of chronic disease. Crit. Rev. Food Sci. Nutr., 44: 275-295.
    CrossRef    PubMed    Direct Link    

  • Zheng, X.Q., L.T. Li, X.L. Liu, X.J. Wang, J. Lin and D. Li, 2006. Production of hydrolysate with antioxidative activity by enzymatic hydrolysis of extruded corn gluten. Applied Microbiol. Biotechnol., 73: 763-770.
    CrossRef    Direct Link    

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