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Pakistan Journal of Nutrition

Year: 2015 | Volume: 14 | Issue: 8 | Page No.: 517-523
DOI: 10.3923/pjn.2015.517.523
Isolation, Purification and Characterization of Lactoferrin from Goat Colostrum Whey
Zainab H. Abbas, Kifah S. Doosh and Nahi Y. Yaseen

Abstract: Lactoferrin is an important protein in many biological applications as a potential cancer treatment agent. In this study, lactoferrin was purified from goat colostrum by ion exchange chromatography through CM-Sephadex C-50 column and gel filtration chromatography through Sephadex G-200 column. The purification fold and yield were (20.83 time and 62.50%) respectively. The purity of lactoferrin to homogeneity was examined by polyacrylamide gel electrophoresis under denaturing conditions (SDS-PAGE) with single bond. Some biochemical characteristics of the purified Lactoferrin were determined, The molecular weight of LF were 80 and 79.50kDa as determined by gel filtration and SDS-PAGE respectively . The percentage of carbohydrate content in goat Lactoferrin was 10.4%, mean while the Iron percentage was 123 ppm and as a saturated percent 7.8%.

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How to cite this article
Zainab H. Abbas, Kifah S. Doosh and Nahi Y. Yaseen, 2015. Isolation, Purification and Characterization of Lactoferrin from Goat Colostrum Whey. Pakistan Journal of Nutrition, 14: 517-523.

Keywords: Lactoferrin, goat colostrum, isolation, purification and characterization

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