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Pakistan Journal of Nutrition

Year: 2015 | Volume: 14 | Issue: 5 | Page No.: 255-258
DOI: 10.3923/pjn.2015.255.258
Chemical Isomerization of Whey Lactose to Lactulose by Using Batch Reaction
Nahla, T.K. and Tariq N. Musa

Abstract: The present study was conducted to evaluate the synthesis of lactulose from whey lactose by using 1, 2 and 3M sodium aluminates as alkaline catalyst in batch reaction system at 30 and 70°C. The effect of different sodium aluminates molarities on the isomerization of lactose to lactulose and the time periods were also investigated. The maximum conversion of lactose to lactulose and the by-products formation have been investigated. The HPLC technique was used to determine whey lactulose, B vitamins and the by-products formation. After 1 h at 70°C, by using 3M of sodium aluminates, the isomerization yield was more than 66%. The present study describes an appropriate method for obtaining sweet whey with acceptable yields of lactulose.

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How to cite this article
Nahla, T.K. and Tariq N. Musa, 2015. Chemical Isomerization of Whey Lactose to Lactulose by Using Batch Reaction. Pakistan Journal of Nutrition, 14: 255-258.

Keywords: Whey lactose, batch isomerization and lactulose

REFERENCES
  • Abboud, M.M., I.H. Aljundi, K.M. Khleifat and S. Dmour, 2010. Biodegradation kinetics and modeling of whey lactose by bacterial hemoglobin VHb-expressing Escherichia coli strain. Biochem. Eng. J., 48: 166-172.
    CrossRef    Direct Link    

  • Alonso, S., M. Rendueles and M. Diaz, 2011. Efficient lactobionic acid production from whey by Pseudomonas taetrolens under pH-shift conditions. Bioresour. Technol., 102: 9730-9736.
    CrossRef    Direct Link    

  • Bertelsen, H., B.B. Jensen and B. Buemann, 1999. D-Tagatose-a novel low-calorie bulk sweetener with prebiotic properties. World Rev. Nutr. Diet., 85: 98-109.
    CrossRef    Direct Link    

  • Schuster-Wolff-Buhring, R., L. Fischer and J. Hinrichs, 2010. Production and physiological action of the disaccharide lactulose. Int. Dairy J., 20: 731-741.
    CrossRef    Direct Link    

  • Cataldi, T.R.I., G. Margiotta, L. Iasi, B. Di Chio, C. Xiloyannis and S.A. Bufo, 2000. Determination of sugar compounds in olive plant extracts by anion-exchange chromatography with pulsed amperometric detection. Anal. Chem., 72: 3902-3907.
    CrossRef    Direct Link    

  • Spalatelu, C., 2012. Biotechnological valorisation of whey. Innov. Roman. Food Biotechnol., 10: 1-8.
    Direct Link    

  • Curiale, V., H. Kolk, H. Pedersen, A. Sanchez-Guevara and T.L. Carlsen et al., 2013. An unpopular geriatric syndrome: Management of chronic constipation in some European countries. Denmark, Estonia, Italy and Luxembourg. Eur. Geriatric Med., 4: 421-426.
    CrossRef    Direct Link    

  • Dominguez, J.R.A., 1984. Glucose isomerase action on acid whey lactose hydrolysate and other sugare. Ph.D. Thesis, Graduate College of the Committee on Nutritional Sciences the University of Arizona, USA.

  • Drakoularakou, A., R. Rastall and G. Gibson, 2011. Functional Foods for the Gut: Probiotics, Prebiotics and Synbiotics. In: Functional Foods: Concept to Product, Saarela, M. (Ed.). 2nd Edn., Woodhead Publishing, Cambridge, UK., ISBN-13: 9781845696900, pp: 449-470

  • Marwaha, S.S. and J.F. Kennedy, 1988. Whey-pollution problem and potential utilization. Int. J. Food Sci. Technol., 23: 323-336.
    CrossRef    Direct Link    

  • Polat, Z., 2009. Integrated approach to whey utilization through natural zeolite adsorption/desorption and fermentation. Ph.D. Thesis, Izmir University, Turkey.

  • Schumann, C., 2002. Medical, nutritional and technological properties of lactulose. An update. Eur. J. Nutr., 41: i17-i25.
    CrossRef    Direct Link    

  • Zokaee, F., T. Kaghazchi, M. Soleimani and A. Zare, 2002. Isomerization of lactose to lactulose using sweet cheese whey ultrafiltrate. J. Chin. Inst. Chem. Eng., 33: 307-313.
    Direct Link    

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