Abstract: A field experiment was conducted to investigate the effect of fungicides on soil native status of Arbuscular mycorrhizal fungi (AMF) and yield of wheat grown in randomized complete block design with three replications during Rabi 2011-12. Three different fungicides commonly practiced by the farmers i-e Chloroflorochlorine, Acrobat and Redimoldgold were applied to crop. The recommended level of Chloroflorochlorine was 1 kg/ha, while that of Acrobat and Redimoldgold were 620 g/ha, respectively. Data revealed that wheat yield, plants nutrients uptakes and soil spores density of AMF and their roots infection intensity decreased with fungicides application as compared with the treatment applied with no fungicides. Highest reduction in grain yield shoot dry matter and plant height were noted with application of Acrobat 50% more than recommended level. Grain and shoot dry matter yields of 3621 and 9212 kg/ha, respectively, produced in this treatment, which 17% less than the treatment of no fungicides application. The application of acrobat 50% more than recommended level also showed decreases maximum in soil total N and AB-DTPA extractable P and Cu, which was significantly (p<0.05), lower by 8 and 40%, respectively over the treatment of no fungicides application. Redimoldgold applied as 50% more than recommended level decreased more AB-DTPA extractable Zn and Mn contents lowered as 48 and 200%, respectively over the treatment of no fungicides application. The highest decreases in plant N and P uptake were observed by the application of Acrobat applied as 50% more than recommended level, lowered as 23 and 30%, respectively over the treatment of no fungicides application. Maximum decrease in spores density and their roots infection intensity were recorded by the application of Acrobat 50% more than recommended level, which were 67 and 50%, respectively, lower than the treatment of no fungicides application. Results suggest that application of fungicides reduces the efficiency of AMF native status in soil and cause reduction in wheat yield and plants nutrients uptake.