Abstract: The study was conducted to investigate the effect of various combinations of phosphorus from organic and inorganic sources on fruit quality of two tomato cultivars i.e., Falcon and Rio Grande for two years. The response of tomato cultivars was assessed to six combinations viz. 0-0, 100-0, 75-25, 50-50, 25-75 and 0-100% of poultry manure (PM) and Triple Super Phosphate (TSP). All the factors (P source, cultivar and year) alone and in combination significantly (p<0.05) affected the fruit quality of tomato fruit. The lowest TSS content (4.10°Brix), Protein (7.05%), Ash (9.80%) and Fat (2.74%) were recorded in fruits of Falcon cultivar in control treatment during 2009. However, lowest fiber content (7.08%) was examined in Rio Grande cultivar in control treatment during 2010. Similarly highest concentration of TSS (4.55°Brix) was noted in Rio Grande cultivar with the application of P source 50-50 ratio. Whereas the fiber content (9.09%) were higher in fruits of Falcon cultivar during 2009. However the protein content (9.75%) and Ash (4.46%) was examined in Rio Grande cultivar with the application of P-source 50-50 and 75-25 ratio, respectively during 2010. The fruit pH and fat content was not significantly affected by the P-source. It was concluded that a combination of PM and TSP at 50-50 and 75-25 ratio may be used as an optimum fertilization dose for obtaining good quality of tomato crop.