Biological Response of Epithelial Cells line (CMT-93) Induced by β-Carotene
Abstract:
β-carotene is the most widely studied carotenoid that has
provitamin A properties beside its great promise for cancer chemoprotective
and human physiological functions. β-carotene can be cleavaged to retinal
by some enzymes in wide variety of cells especially in intestinal cells. The
retinal is further metabolized to retinoic acid by Aldehyde Dehydrogenase (ALDH)
family of enzymes. In this study we use CMT-93 cell line as a cell model of
intestinal epithelial cell to study the effect of β-carotene on cells morphology,
proliferation and Aldehyde Dehydrogenase (ALDH) gene expression. These cells
were grown in DMEM supplemented with 10% heat-inactivated Fecal Bovine Serum
(FBS), 4 mM L-glutamine, 50 U/ml penicillin and 50 mg/ml streptomycin. Tetrahydrofuran
(THF) (1.25%) was used to solve β-carotene (Sigma). Final concentration
of β-carotene in medium for proliferation were 0.5, 1.0, 1.5 and 5.0 μM.
The results showed that in higher concentration (1.5 μM), β-carotene
can alter the morphologycal structure of monolayer epithelial cells of CMT-93.
Meanwhile, β-carotene in supra physiological
concentration (5.0 μM) significantly (p<0.05) decreased proliferation,
exhibited ALDH1A2 gene expression and changed morphological structure.
How to cite this article
Katrin Roosita, Rimbawan Sukandar Martopuro, Ita Djuwita, M. Rizal Damanik, Clara M. Kusharto and Fumito Tani, 2013. Biological Response of Epithelial Cells line (CMT-93) Induced by β-Carotene. Pakistan Journal of Nutrition, 12: 615-619.
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