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Pakistan Journal of Nutrition

Year: 2013 | Volume: 12 | Issue: 5 | Page No.: 427-432
DOI: 10.3923/pjn.2013.427.432
Optimization of Growth and Amylase Production by Aspergillus flavus Grown on Some Agricultural Raw Materials in Nigeria
Oyewale Mojeed Oladapo

Abstract: Aspergillus flavus exhibited high growth and amylase production. Various Polysaccharides, including soluble starch, sorghum, cassava peel and maize induces high amylase production with maximal amylase activity at 2% W/V. An initial linear increase in amylase activity with increase in substrate concentration was obtained up to 2% W/V. An antagonistic relationship was obtained between amylase activity and substrate concentrations higher than 2% W/V, in the culture filtrate of Aspergillus flavus grown in shake flasks. The maximum growth and amylase production was obtained on the sixth day of incubation at a pH of 7.0 temperature of 29±1°C and 80 rpm. It is concluded from this study that cassava peel as well as sorghum may be an alternative carbon substrate for large scale cultivation of A. flavus for amylase production.

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How to cite this article
Oyewale Mojeed Oladapo , 2013. Optimization of Growth and Amylase Production by Aspergillus flavus Grown on Some Agricultural Raw Materials in Nigeria. Pakistan Journal of Nutrition, 12: 427-432.

Keywords: Soluble starch, sorghum, cassava peel maize, amylase activity, growth, substrate concentration and Aspergillus flavus

REFERENCES

  • Akinyosoye, F.A. and J.A. Akinyanju, 1989. Effects of different Carbon sources on growth and sporulation of Geotrichum candidum. Nig. J. Bot., 2: 89-95.


  • Ali, S., S. Mahmood, R. Alam and Z. Hossain, 1989. Culture condition for production of glucoamylase from rice bran by Aspergillus terreus. MIRCEN J. Applied. Microbiol. Biotechnol., 5: 525-532.
    CrossRef    


  • Antranikian, G., 1992. Microbial Degradation of Starch. In: Microbial Degradation of Natural Products, Winkel, G. (Ed.). VCH, Germany, pp: 27-51


  • Bernfeld, P., 1955. Amylases, α and β. In: Methods in Enzymology, Colowick S.P. and N.O. Kaplan (Eds.). Vol. 1, Academic Press, New York, USA., pp: 149-158


  • Borkar, S. and S. Bhosle, 2003. Alkaliphiles Microbes in Alkaline Environments in Advances in Microbiology. P.C. Triveddi Scientific Publishers, India


  • Demot, R. and H.C. Verachtert, 1987. Purification and crystallization of extracellular amylolytic enzymes from yeast Filobasidium capsuligenum. Applied Environ. Microbiol., 50: 1474-1482.


  • Ettalibi, M. and J.C. Baratti, 1988. Isolation and characterization of an amylolytic yeast: Candida edax. MIRCEN. J. Applied Microbiol. Biotech., 6: 193-202.
    CrossRef    


  • Fawole, M.O., 1986. The place of enzymology in industrial development. Proceedings of the 2nd Annual Conference of the Biotechnology Society of Nigeria, May 16 18, 1986, University of Ilorin, Ilorin, Nigeria -.


  • Fergus, C.L., 1969. The production of amylase by some thermophilic fungi. Mycologia, 61: 1171-1175.
    Direct Link    


  • Garg, S.K. and H.H. Doelle, 1989. Optimization of cassava starch conversion to glucose by Rhizopus oligosporus. MIRCEN J. Applied Microbiol. Biotechnol., 5: 297-305.
    CrossRef    


  • Lagzouli, M., M. Zakaria, A. Ali, W. Senhaji and M. Ouhssine et al., 2007. Optimization of growth and extracellular glucoamylase production by Candida famata isolate. Afr. J. Biotechnol., 6: 2590-2595.
    Direct Link    


  • Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
    CrossRef    PubMed    Direct Link    


  • Mountfort, D.O. and R.A. Asher, 1988. Production of α-amylase by the ruminal anaerobic fungus (Neocaellimastix frontalis). Applied Environ. Microbiol., 54: 2293-2299.
    PubMed    


  • Ogundero, V.W., 1979. Amylase and cellulase activities of thermophilic fungi causing deterioration of Tobacco products in Nigeria. Mycopathologia, 69: 131-135.
    CrossRef    


  • Ogundero, V.W., 1982. The production and activity of Hydrolytic exoenzymes by toxigenic species of Aspergillus from Garri. Nig. J. Sci., 16: 11-20.


  • Ogundero, V.W., 1982. Cellulose hydrolysis by thermophilic fungi from composting plant materials. Nig. J. Sci., 16: 83-93.


  • Oyewale, M.O., 2006. Pharmaceutical Microbiology, for the Tropics. 2nd Edn., Post Harvest and Biotechnology Research Centre, Osogbo, pp: 107-124


  • Oyewale, M.O., 2010. Growth and amylase production by Aspergillus flavus grown on commercial soluble starch and cassava peel. J. Pure Applied Sci., 7: 53-56.


  • Pestana, F. and F.J. Castillo, 1985. Glucoamylase production by Aspergillus awamori on rice flour medium and partial characterization of the enzyme. MIRECN J. Applied Microbiol. Biotechnol., 1: 225-237.
    CrossRef    


  • Rai, M.K. and S.K. Deshmukh, 2005. Fungi: Diversity and Biotechnology. Scientific Publishers, India, ISBN: 9788172334031, Pages: 510


  • Kumar, S. and T. Satyanarayana, 2005. Starch Hydrolyzing Enzymes of Thermaphilic Moulds. In: Fungi: Diversity and Biotechnology, Rai, M.K. and S.K. Deshmukh (Eds.). Scientific Publishers, India, pp: 229-250

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