Gestational Outcome in Rats That Consumed Aqueous Extract of Hibiscus sabdariffa During Pregnancy
Abstract:
The present study was designed to investigate the effect of consumption of aqueous extract of Hibiscus sabdariffa (HS) during pregnancy on fluid and food intake, weight gain during pregnancy, length of gestation, litter size and birth weight. Eighteen in-bred virgin female Sprague-Dawley rats aged between 10-12 weeks and weighing 125 ± 5.5g (mean ± SEM) with two consecutive regular 4-day estrus cycle were used for this study. These rats were randomly assigned to one of three groups on gestational day one. Group A (control) were given tap water to drink throughout pregnancy, while groups B and C were given 0.6g-extract/100ml and 1.8g-extract/100ml respectively to drink throughout pregnancy. All groups had normal rat chow and their respective drinking solution ad libitum. Mean fluid and food intake and total pregnancy weight gain were significantly reduced in the HS group. There was no significant difference in length of gestation among the various groups but there was a significant reduction in litter size and a significant increase in litter birth weight in HS groups (B and C) compared with control group (A). There were no apparent malformations at birth.
How to cite this article
E.E. Iyare and O.A. Adegoke, 2011. Gestational Outcome in Rats That Consumed Aqueous Extract of Hibiscus sabdariffa During Pregnancy. Pakistan Journal of Nutrition, 10: 350-354.
REFERENCES
Adegunloye, B.J., J.O. Omoniyi, O.A. Owolabi, O.P. Ajagbonna, O.A. Sofola and H.A. Coker, 1996. Mechanisms of the blood pressure lowering effect of the calyx extract of Hibiscus sabdariffa in rats. Afr. J. Med. Med. Sci., 25: 235-238.
PubMed Direct Link
Adegunloye, B.J., J.O. Omoniyi, O.A. Owolabi, O.P. Ajagbonna and O.A. Sofola, 1993. Petals extract of Hibiscus sabdariffa elicits relaxation of rat aorta by stabilizing the mem-brane and stimulating Na þ-K þ-ATPase. Nig. J. Physiol. Sci., 9: 74-79.
Adigun, M.O., O.D. Ogundipe, J.I. Anetor and A.O. Odetunde, 2006. Dose-dependent changes in some haematological parameters during short-term administration of Hibiscus sabdariffa Calyx aqueous extract (zobo) in Wistar albino rats. Afr. J. Med. Med. Sci., 35: 73-77.
PubMed
Ajay, M., H.J. Chai, A.M. Mustafa, A.H. Gilani and M.R. Mustafa, 2007. Mechanisms of the anti-hypertensive effect of Hibiscus sabdariffa L. calyces. J. Ethnopharmacol., 109: 388-393.
CrossRef PubMed Direct Link
Alarcon-Aguilar, F.J., A. Zamilpa, M.D. Perez-Garcia, J.C. Almanza-Perez and E. Romero-Nunez et al., 2007. Effect of Hibiscus sabdariffa on obesity in MSG mice. J. Ethnopharmacol., 114: 66-71.
CrossRef
Appel, S.D., 2003. Red sorrel, Hibiscus sabdariffa: The other cranberry. Plants Gardens News, 18: 1-2.
Armitage, J.A., L. Lakasing, P.D. Taylor, A.A. Balachandran and R.I. Jensen et al., 2005. Developmental programming of aortic and renal structure in offspring of rats fed fat-rich diets in pregnancy. J. Physiol., 565: 171-184.
CrossRef
Armitage, J.A., P.D. Taylor and L. Poston, 2005. Experimental models of developmental programming: consequences of exposure to an energy rich diet during development. J. Physiol., 565: 3-8.
PubMed
Barker, D.J., 2000. In utero programming of cardiovascular disease. Theriogenology, 53: 555-574.
PubMed
Barker, D.J., C.N. Hales, C.H. Fall, C. Osmond, K. Phipps and P.M. Clark, 1993. Type 2 (non-insulin dependent) diabetes mellitus, hypertension and hyperlipidemia (syndrome X): Relation to reduced fetal growth. Diabetologia, 36: 62-67.
Ceesay, S.M., A.M. Prentice, T.J. Cole, F. Foord, L.T. Weaver, E.M. Poskitt and R.G. Whitehead, 1997. Effects on birth weight and perinatal mortality of maternal dietary supplements in rural Gambia: 5 year randomised controlled trial. Br. Med. J., 315: 786-790.
Clydesdale, F.M.C., 1979. Roselle (H.S.L.) anthocyanins as colourants for beverages and gelatin deserts. J. Food Prot., 42: 204-267.
Dafallah, A.A. and Z. Al-Mustafa, 1996. Investigation of the anti-inflammatory activity of Acacia nilotica and Hibiscus sabdariffa. Am. J. Chin. Med., 24: 263-269.
CrossRef PubMed Direct Link
Duke, C.T. and F.J. Francis, 1973. Anthocyanins of roselle (H.S.L.). J. Food Sci., 38: 810-812.
Farombi, E.O. and O.O. Ige, 2007. Hypolipidemic and antioxidant effects of ethanolic extract from dried calyx of Hibiscus sabdariffa in alloxan-induced diabetic rats. Fundam. Clin. Pharmacol., 21: 601-609.
CrossRef Direct Link
Fowden, A.L. and A.J. Forhead, 2004. Endocrine mechanisms of intrauterine programming. Reproduction, 127: 515-526.
CrossRef Direct Link
Fowden, A.L., J.W. Ward, F.P.B. Wooding, A.J. Forhead and M. Constancia, 2006. Programming placental nutrient transport capacity. J. Physiol., 572: 5-15.
CrossRef Direct Link
Fuleki, T. and F.J. Francis, 1968. Determination of total anthocyanin and degradation index. J. Food Sci., 33: 78-82.
Gluckman, P.D. and M.A. Hanson, 2004. Developmental origins of disease paradigm: A mechanistic and evolutionary perspective. Pediatr. Res., 56: 311-317.
CrossRef
Gluckman, P.D. and M.A. Hanson, 2004. The developmental origins of the metabolic syndrome. Trends. Endocrinol. Metab., 15: 183-187.
CrossRef
Faraji, M.H. and A.H.H. Tarkhani, 1999. The effect of sour tea (Hibiscus sabdariffa) on essential hypertension. J. Ethnopharmacol., 65: 231-236.
CrossRef PubMed
Herrera-Arellano, A., J. Miranda-Sanchez, P. Avila-Castro, S. Herrera-Alvarez and J.E. Jimenez-Ferrer et al., 2007. Clinical effects produced by a standardized herbal medicinal product of Hibiscus sabdariffa on patients with hypertension. A randomized, double-blind, lisinopril-controlled clinical trial. Planta Med., 73: 6-12.
CrossRef PubMed Direct Link
Hilakivi-Clarke, L., E. Cho and R. Clarke, 1998. Maternal genistein exposure mimics the effects of estrogen on mammary gland development in female mouse offspring. Oncol. Rep., 5: 609-616.
Direct Link
Hirunpanich, V., A. Utaipat, N.P. Morales, N. Bunyapraphatsara, H. Sato, A. Herunsale and C. Suthisisang, 2006. Hypocholesterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa L. in hypercholesterolemic rats. J. Ethnopharmacol., 103: 252-260.
CrossRef
Iyare, E.E. and A.O. Adegoke, 2008. Postnatal weight gain and onset of puberty in rats exposed to aqueous extract of Hibiscus sabdariffa in utero. Pak. J. Nutr., 7: 98-101.
CrossRef Direct Link
Jain, S.K., R. Wise, K. Yanamandra, R. Dhanireddy and J.A. Jr. Bocchini, 2008. The effect of maternal and cord-blood vitamin C, vitamin E and lipid peroxide levels on newborn birth weight. Mol. Cell Biochem., 309: 217-221.
Direct Link
Kim, J.K., H. So, M.J. Youn, H.J. Kim and Y. Kim et al., 2007. Hibiscus sabdariffa L. water extract inhibits the adipocyte differentiation through the PI3-K and MAPK pathway. J. Ethnopharmacol., 114: 260-267.
CrossRef
Lans, C.A., 2006. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. J. Ethnobiol. Ethnomed., Vol. 2.
CrossRef
Lesage, J., B. Blondeau, M. Grino, B. Breant and J.P. Dupouy, 2001. Maternal undernutrition during late gestation induces fetal overexposure to glucocorticoids and intrauterine growth retardation and disturbs the hypothalamo-pituitary adrenal axis in the newborn rat. Endocrinology, 142: 1692-1702.
CrossRef PubMed
Mojiminiyi, F.B.O., B.J. Adegunloye, Y.A. Egbeniyi and R.U. Okolo, 2000. An investigation of the diuretic effect of an aqueous extract of the petal of Hibiscus sabdariffa. J. Med. Sci., 2: 77-80.
Mojiminiyi, F.B.O., M. Dikko, B.Y. Muhammad, P.D. Ojobor and O.P. Ajagbonna et al., 2007. Antihypertensive effect of an aqueous extract of the calyx of Hibiscus sabdariffa. Fitoterapia, 78: 292-297.
CrossRef Direct Link
Morton, J.F., 1987. Roselle. In: Fruits of Warm Climates, Dowling, C.F. (Ed). Media Inc., Greensboro NC., pp: 281-286
Nivsarkar, M., M. Patel, H. Padh, C. Bapu and N. Shrivastava, 2005. Blastocyst implantation failure in mice due to nonreceptive endometrium: Endometrial alterations by Hibiscus rosa-sinensis leaf extract. Contraception, 71: 227-230.
CrossRef PubMed Direct Link
Obiefuna, P.C.M., O.A. Owolabi, B.J. Adegunloye, I.P. Obiefuna and O.A. Sofola, 1994. The petal extract of Hibiscus sabdariffa produces relaxation of isolated rat aorta. Int. J. Pharmacol., 34: 69-74.
Direct Link
Odigie, I.P., R.R. Ettarh and S.A. Adigun, 2003. Chronic administration of aqueous extract of Hibiscus sabdariffa attenuates hypertension and reverses cardiac hypertrophy in 2K-1C hypertensive rats. J. Ethnopharmacol., 86: 181-185.
CrossRef Direct Link
Ojokoh, A.O., 2006. Roselle (Hibiscus sabdariffa) calyx diet and histopathological changes in liver of albino rats. Pak. J. Nutr., 5: 110-113.
CrossRef Direct Link
Oliver, B., 1962. Medicinal Plants in Nigeria. Nigerian College of Arts Science and Technology, Ibadan, pp: 16-42
Onyenekwe, P.C., E.O. Ajani, D.A. Ameh and K.S. Gamaniel, 1999. Antihypertensive effect of roselle (Hibiscus sabdariffa) calyx infusion in spontaneously hypertensive rats and a comparison of its toxicity with that in Wistar rats. Cell. Biochem. Funct., 17: 199-206.
CrossRef PubMed Direct Link
Osgerby, J.C., D.C. Wathes, D. Howard and T.S. Gadd, 2002. The effect of maternal undernutrition on ovine fetal growth. J. Endocrinol., 173: 131-141.
PubMed Direct Link
Perry, L.M., 1980. Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses. 1st Edn., The MIT Press, Cambridge, UK., ISBN-13: 978-0262160766, Pages: 632
Phillips, D.I.W., D.J.P. Barker, C.H.D. Fall, J.R. Seckl, C.B. Whorwood, P.J. Wood and B.R. Walker, 1998. Elevated plasma cortisol concentrations: A link between low birth weight and the insulin resistance syndrome? J. Clin. Endocrinol. Metab., 83: 757-760.
CrossRef PubMed Direct Link
Ross, M.G. and M. Desai, 2005. Gestational programming: population survival effects of drought and famine during pregnancy. Am. J. Physiol. Regul. Integr. Comp. Physiol., 288: 25-33.
CrossRef
Seckl, J.R., 1998. Physiologic programming of the fetus. Clin. Perinatol., 25: 939-962.
PubMed
Wang, M.S., H. Shi, K.S. Wang and M.M. Reidenberg, 2002. Inhibition of 11β-hydroxysteroid dehydrogenase in guinea pig kidney by three bioflavonoids and their interactions with gossypol. Acta Pharmacol. Sin., 23: 92-96.
PubMed
Watt, J.M. and M.G. Breyer-Brandwijk, 1962. The Medicinal and Poisonous Plants of Southern and Eastern Africa. 2nd Edn., E and S Liningstone Ltd., London, UK., Pages: 1457
Woodall, S.M., B.H. Breier, B.M. Johnston and P.D. Gluckman, 1996. A model of intrauterine growth retardation caused by chronic maternal undernutrition in the rat: Effects on the somatotrophic axis and postnatal growth. J. Endocrinol., 150: 231-242.
CrossRef Direct Link
Wu, X., T. Iguchi, N. Itoh, K. Okamoto, T. Takagi, K. Tanaka and T. Nakanishi, 2008. Ascorbic acid transported by sodium-dependent vitamin C transporter 2 stimulates steroidogenesis in human choriocarcinoma cells. Endocrinol. J., 149: 73-83.
Direct Link
Yajnik, C., 2006. Nutritional control of foetal growth. Nutr. Rev., 64: S50-S51.
CrossRef
Carvajal-Zarrabal,O.,S.M. Waliszewski, D.M. Barradas-Dermitz, Z. Orta-Flores and P.M. Hayward-Jones et al., 2005. The consumption of Hibiscus sabdariffa dried calyx ethanolic extract reduced lipid profile in rats. Plant Foods Hum. Nutr., 60: 153-159.
CrossRef
© Science Alert. All Rights Reserved